Experiment: Flask growth in 60 uE Light-Dark 12:12
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mutS and gltA are separated by 3 nucleotides gltA and sixA are separated by 134 nucleotides sixA and Synpcc7942_0614 are separated by 3 nucleotides
Synpcc7942_0611: mutS - recombination and DNA strand exchange inhibitor protein, at 599,815 to 602,205
mutS
Synpcc7942_0612: gltA - methylcitrate synthase, at 602,209 to 603,369
gltA
Synpcc7942_0613: sixA - phosphohistidine phosphatase, SixA, at 603,504 to 603,992
sixA
Synpcc7942_0614: Synpcc7942_0614 - hypothetical protein, at 603,996 to 605,675
_0614
Position (kb) 602
603
604 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 601.272 kb on + strand, within mutS at 601.272 kb on + strand, within mutS at 601.272 kb on + strand, within mutS at 601.280 kb on - strand, within mutS at 601.280 kb on - strand, within mutS at 601.295 kb on + strand at 601.298 kb on - strand, within mutS at 601.298 kb on - strand, within mutS at 601.333 kb on - strand, within mutS at 601.355 kb on - strand, within mutS at 601.426 kb on - strand, within mutS at 601.453 kb on - strand at 601.453 kb on - strand, within mutS at 601.476 kb on + strand, within mutS at 601.479 kb on - strand, within mutS at 601.515 kb on + strand, within mutS at 601.515 kb on + strand, within mutS at 601.515 kb on + strand, within mutS at 601.523 kb on - strand, within mutS at 601.533 kb on - strand, within mutS at 601.571 kb on - strand, within mutS at 601.617 kb on + strand, within mutS at 601.630 kb on + strand, within mutS at 601.638 kb on - strand, within mutS at 601.648 kb on - strand, within mutS at 601.686 kb on + strand, within mutS at 601.717 kb on + strand, within mutS at 601.779 kb on + strand, within mutS at 601.793 kb on - strand, within mutS at 601.823 kb on + strand, within mutS at 601.823 kb on + strand, within mutS at 601.831 kb on - strand, within mutS at 601.844 kb on + strand, within mutS at 601.885 kb on - strand, within mutS at 601.923 kb on + strand, within mutS at 601.931 kb on - strand, within mutS at 601.981 kb on + strand at 601.982 kb on + strand at 601.990 kb on - strand at 601.990 kb on - strand at 601.990 kb on - strand at 602.008 kb on - strand at 602.038 kb on - strand at 602.112 kb on + strand at 602.154 kb on + strand at 602.192 kb on + strand at 602.199 kb on + strand at 602.200 kb on + strand at 602.200 kb on + strand at 602.202 kb on - strand at 602.208 kb on - strand at 603.455 kb on - strand at 603.490 kb on + strand at 603.510 kb on - strand at 603.510 kb on - strand at 603.510 kb on - strand at 603.510 kb on - strand at 603.529 kb on + strand at 603.569 kb on - strand, within sixA at 603.583 kb on - strand, within sixA at 603.603 kb on - strand, within sixA at 603.603 kb on - strand, within sixA at 603.615 kb on + strand, within sixA at 603.615 kb on + strand, within sixA at 603.615 kb on + strand, within sixA at 603.618 kb on - strand, within sixA at 603.623 kb on - strand, within sixA at 603.623 kb on - strand, within sixA at 603.641 kb on + strand, within sixA at 603.661 kb on - strand, within sixA at 603.661 kb on - strand, within sixA at 603.661 kb on - strand, within sixA at 603.687 kb on + strand, within sixA at 603.687 kb on + strand, within sixA at 603.732 kb on + strand, within sixA at 603.780 kb on + strand, within sixA at 603.825 kb on + strand, within sixA at 603.828 kb on - strand, within sixA at 603.838 kb on + strand, within sixA at 603.845 kb on - strand, within sixA at 603.850 kb on + strand, within sixA at 603.905 kb on + strand, within sixA at 603.913 kb on + strand, within sixA at 603.994 kb on + strand at 604.037 kb on - strand at 604.052 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Flask growth in 60 uE Light-Dark 12:12 remove 601,272 + mutS Synpcc7942_0611 0.61 +1.3 601,272 + mutS Synpcc7942_0611 0.61 +1.7 601,272 + mutS Synpcc7942_0611 0.61 +0.5 601,280 - mutS Synpcc7942_0611 0.61 +0.6 601,280 - mutS Synpcc7942_0611 0.61 +2.0 601,295 + +1.0 601,298 - mutS Synpcc7942_0611 0.62 +1.9 601,298 - mutS Synpcc7942_0611 0.62 +0.3 601,333 - mutS Synpcc7942_0611 0.63 +0.4 601,355 - mutS Synpcc7942_0611 0.64 +0.7 601,426 - mutS Synpcc7942_0611 0.67 +1.0 601,453 - +1.2 601,453 - mutS Synpcc7942_0611 0.69 +0.8 601,476 + mutS Synpcc7942_0611 0.69 +1.2 601,479 - mutS Synpcc7942_0611 0.70 -0.9 601,515 + mutS Synpcc7942_0611 0.71 +1.4 601,515 + mutS Synpcc7942_0611 0.71 +1.0 601,515 + mutS Synpcc7942_0611 0.71 +1.8 601,523 - mutS Synpcc7942_0611 0.71 +0.5 601,533 - mutS Synpcc7942_0611 0.72 +1.5 601,571 - mutS Synpcc7942_0611 0.73 +1.2 601,617 + mutS Synpcc7942_0611 0.75 +1.1 601,630 + mutS Synpcc7942_0611 0.76 +1.4 601,638 - mutS Synpcc7942_0611 0.76 -0.7 601,648 - mutS Synpcc7942_0611 0.77 +0.6 601,686 + mutS Synpcc7942_0611 0.78 +1.3 601,717 + mutS Synpcc7942_0611 0.80 -0.4 601,779 + mutS Synpcc7942_0611 0.82 +1.3 601,793 - mutS Synpcc7942_0611 0.83 +2.9 601,823 + mutS Synpcc7942_0611 0.84 +1.2 601,823 + mutS Synpcc7942_0611 0.84 +0.9 601,831 - mutS Synpcc7942_0611 0.84 +0.8 601,844 + mutS Synpcc7942_0611 0.85 -2.8 601,885 - mutS Synpcc7942_0611 0.87 +0.7 601,923 + mutS Synpcc7942_0611 0.88 +1.3 601,931 - mutS Synpcc7942_0611 0.88 -0.7 601,981 + -0.6 601,982 + -0.1 601,990 - -0.5 601,990 - +0.8 601,990 - +1.7 602,008 - -0.4 602,038 - +1.7 602,112 + +2.7 602,154 + +1.9 602,192 + -2.1 602,199 + +0.8 602,200 + +0.9 602,200 + +0.4 602,202 - +0.3 602,208 - -1.2 603,455 - -0.0 603,490 + +1.2 603,510 - +0.7 603,510 - -0.3 603,510 - +0.2 603,510 - +0.3 603,529 + -2.9 603,569 - sixA Synpcc7942_0613 0.13 +1.8 603,583 - sixA Synpcc7942_0613 0.16 +0.2 603,603 - sixA Synpcc7942_0613 0.20 +0.9 603,603 - sixA Synpcc7942_0613 0.20 -1.6 603,615 + sixA Synpcc7942_0613 0.23 -1.1 603,615 + sixA Synpcc7942_0613 0.23 +0.6 603,615 + sixA Synpcc7942_0613 0.23 -0.7 603,618 - sixA Synpcc7942_0613 0.23 -1.3 603,623 - sixA Synpcc7942_0613 0.24 -1.8 603,623 - sixA Synpcc7942_0613 0.24 -0.3 603,641 + sixA Synpcc7942_0613 0.28 +0.8 603,661 - sixA Synpcc7942_0613 0.32 -0.7 603,661 - sixA Synpcc7942_0613 0.32 -0.0 603,661 - sixA Synpcc7942_0613 0.32 +0.3 603,687 + sixA Synpcc7942_0613 0.37 -0.3 603,687 + sixA Synpcc7942_0613 0.37 -0.9 603,732 + sixA Synpcc7942_0613 0.47 +0.4 603,780 + sixA Synpcc7942_0613 0.56 -0.6 603,825 + sixA Synpcc7942_0613 0.66 -0.7 603,828 - sixA Synpcc7942_0613 0.66 +0.2 603,838 + sixA Synpcc7942_0613 0.68 -0.1 603,845 - sixA Synpcc7942_0613 0.70 -0.2 603,850 + sixA Synpcc7942_0613 0.71 +0.6 603,905 + sixA Synpcc7942_0613 0.82 +0.2 603,913 + sixA Synpcc7942_0613 0.84 +0.4 603,994 + +0.9 604,037 - -0.8 604,052 - +0.3
Or see this region's nucleotide sequence