Experiment: Flask growth in 60 uE Light-Dark 12:12
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_0402 and Synpcc7942_0403 are separated by 1 nucleotides Synpcc7942_0403 and prfB are separated by 17 nucleotides
Synpcc7942_0402: Synpcc7942_0402 - hypothetical protein, at 396,872 to 397,396
_0402
Synpcc7942_0403: Synpcc7942_0403 - hypothetical protein, at 397,398 to 397,577
_0403
Synpcc7942_0404: prfB - peptide chain release factor 2, at 397,595 to 398,509
prfB
Position (kb)
396
397
398 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 395.898 kb on + strand at 395.903 kb on + strand at 395.906 kb on - strand at 395.906 kb on - strand at 396.013 kb on + strand at 396.418 kb on + strand at 396.738 kb on + strand at 396.738 kb on + strand at 396.746 kb on - strand at 396.746 kb on - strand at 396.750 kb on + strand at 396.757 kb on - strand at 396.758 kb on + strand at 396.771 kb on + strand at 396.787 kb on + strand at 396.787 kb on + strand at 396.787 kb on + strand at 396.795 kb on - strand at 396.797 kb on + strand at 396.807 kb on + strand at 396.815 kb on - strand at 396.815 kb on - strand at 396.815 kb on - strand at 396.819 kb on + strand at 396.825 kb on + strand at 396.849 kb on + strand at 396.870 kb on + strand at 396.878 kb on - strand at 396.878 kb on - strand at 396.893 kb on - strand at 396.912 kb on - strand at 396.917 kb on + strand at 396.925 kb on - strand, within Synpcc7942_0402 at 397.404 kb on + strand at 397.408 kb on + strand at 397.425 kb on + strand, within Synpcc7942_0403 at 397.439 kb on + strand, within Synpcc7942_0403 at 397.448 kb on - strand, within Synpcc7942_0403 at 397.448 kb on - strand, within Synpcc7942_0403 at 397.483 kb on - strand, within Synpcc7942_0403 at 397.495 kb on - strand, within Synpcc7942_0403 at 397.500 kb on - strand, within Synpcc7942_0403 at 397.508 kb on + strand, within Synpcc7942_0403 at 397.561 kb on + strand at 397.592 kb on + strand at 397.600 kb on - strand at 397.600 kb on - strand at 397.600 kb on - strand at 397.600 kb on - strand at 397.600 kb on - strand at 397.615 kb on + strand at 397.634 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Flask growth in 60 uE Light-Dark 12:12 remove 395,898 + -1.2 395,903 + -0.2 395,906 - -0.2 395,906 - -1.2 396,013 + +1.5 396,418 + -0.9 396,738 + +0.3 396,738 + +1.6 396,746 - -0.5 396,746 - -0.1 396,750 + -0.1 396,757 - -0.0 396,758 + +0.1 396,771 + -0.4 396,787 + +1.0 396,787 + -0.3 396,787 + -0.5 396,795 - -3.2 396,797 + -0.7 396,807 + -1.8 396,815 - -1.7 396,815 - -0.8 396,815 - -0.6 396,819 + -0.4 396,825 + +0.8 396,849 + -0.2 396,870 + -0.6 396,878 - -0.8 396,878 - +0.5 396,893 - -1.1 396,912 - +0.5 396,917 + -2.1 396,925 - Synpcc7942_0402 0.10 -2.6 397,404 + +0.5 397,408 + +0.3 397,425 + Synpcc7942_0403 0.15 +1.2 397,439 + Synpcc7942_0403 0.23 +1.3 397,448 - Synpcc7942_0403 0.28 +1.2 397,448 - Synpcc7942_0403 0.28 +1.1 397,483 - Synpcc7942_0403 0.47 -1.7 397,495 - Synpcc7942_0403 0.54 -0.0 397,500 - Synpcc7942_0403 0.57 -1.2 397,508 + Synpcc7942_0403 0.61 +1.2 397,561 + +1.2 397,592 + -1.2 397,600 - -0.9 397,600 - -1.0 397,600 - -0.2 397,600 - +0.2 397,600 - -0.7 397,615 + +1.6 397,634 - +0.5
Or see this region's nucleotide sequence