Experiment: Flask growth in 60 uE Light-Dark 12:12
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt clpC and rimI are separated by 143 nucleotides rimI and lysA are separated by 47 nucleotides
Synpcc7942_0260: clpC - ATPase, at 255,961 to 258,435
clpC
Synpcc7942_0261: rimI - ribosomal-protein-alanine acetyltransferase, at 258,579 to 259,178
rimI
Synpcc7942_0262: lysA - diaminopimelate decarboxylase, at 259,226 to 260,620
lysA
Position (kb) 258
259
260 Strain fitness (log2 ratio) -2
-1
0
1 at 258.555 kb on + strand at 258.555 kb on + strand at 258.556 kb on + strand at 258.570 kb on + strand at 258.581 kb on + strand at 258.589 kb on - strand at 258.591 kb on + strand at 258.599 kb on + strand at 258.599 kb on - strand at 258.627 kb on - strand at 258.647 kb on + strand, within rimI at 258.680 kb on - strand, within rimI at 258.685 kb on + strand, within rimI at 258.690 kb on - strand, within rimI at 258.693 kb on - strand, within rimI at 258.777 kb on - strand, within rimI at 258.777 kb on - strand, within rimI at 258.805 kb on - strand, within rimI at 258.813 kb on - strand, within rimI at 258.867 kb on + strand, within rimI at 258.868 kb on + strand, within rimI at 258.870 kb on - strand, within rimI at 258.918 kb on + strand, within rimI at 258.931 kb on - strand, within rimI at 258.935 kb on + strand, within rimI at 258.940 kb on - strand, within rimI at 258.960 kb on + strand, within rimI at 258.990 kb on + strand, within rimI at 258.992 kb on - strand, within rimI at 258.998 kb on - strand, within rimI at 258.998 kb on - strand, within rimI at 259.050 kb on + strand, within rimI at 259.123 kb on + strand at 259.123 kb on + strand at 259.123 kb on + strand at 259.141 kb on - strand at 259.148 kb on + strand at 259.163 kb on + strand at 259.163 kb on + strand at 259.168 kb on + strand at 259.170 kb on + strand at 259.171 kb on - strand at 259.178 kb on - strand at 259.178 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Flask growth in 60 uE Light-Dark 12:12 remove 258,555 + -1.5 258,555 + +0.0 258,556 + -0.5 258,570 + +1.3 258,581 + -0.4 258,589 - +0.5 258,591 + +0.5 258,599 + +0.6 258,599 - -0.3 258,627 - +0.3 258,647 + rimI Synpcc7942_0261 0.11 -0.7 258,680 - rimI Synpcc7942_0261 0.17 +0.1 258,685 + rimI Synpcc7942_0261 0.18 -0.4 258,690 - rimI Synpcc7942_0261 0.18 -1.8 258,693 - rimI Synpcc7942_0261 0.19 -1.0 258,777 - rimI Synpcc7942_0261 0.33 -1.0 258,777 - rimI Synpcc7942_0261 0.33 -0.2 258,805 - rimI Synpcc7942_0261 0.38 +0.1 258,813 - rimI Synpcc7942_0261 0.39 +1.7 258,867 + rimI Synpcc7942_0261 0.48 -0.7 258,868 + rimI Synpcc7942_0261 0.48 -0.3 258,870 - rimI Synpcc7942_0261 0.48 -0.1 258,918 + rimI Synpcc7942_0261 0.56 -2.3 258,931 - rimI Synpcc7942_0261 0.59 -0.1 258,935 + rimI Synpcc7942_0261 0.59 -0.8 258,940 - rimI Synpcc7942_0261 0.60 -1.7 258,960 + rimI Synpcc7942_0261 0.64 -0.9 258,990 + rimI Synpcc7942_0261 0.69 -1.7 258,992 - rimI Synpcc7942_0261 0.69 -0.7 258,998 - rimI Synpcc7942_0261 0.70 +0.1 258,998 - rimI Synpcc7942_0261 0.70 -0.8 259,050 + rimI Synpcc7942_0261 0.79 -1.1 259,123 + -0.6 259,123 + -0.3 259,123 + -0.3 259,141 - -2.0 259,148 + +0.1 259,163 + -0.3 259,163 + -0.4 259,168 + -2.3 259,170 + -0.8 259,171 - +0.7 259,178 - -0.5 259,178 - -2.2
Or see this region's nucleotide sequence