Experiment: Flask growth in 60 uE Light-Dark 12:12
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_0255 and ama are separated by 89 nucleotides ama and Synpcc7942_0257 overlap by 4 nucleotides Synpcc7942_0257 and Synpcc7942_0258 are separated by 48 nucleotides
Synpcc7942_0255: Synpcc7942_0255 - ATPase, at 251,503 to 252,783
_0255
Synpcc7942_0256: ama - Peptidase M20D, amidohydrolase, at 252,873 to 254,099
ama
Synpcc7942_0257: Synpcc7942_0257 - Protein of unknown function DUF92, transmembrane, at 254,096 to 254,845
_0257
Synpcc7942_0258: Synpcc7942_0258 - hypothetical protein, at 254,894 to 255,334
_0258
Position (kb) 252
253
254
255 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 251.883 kb on + strand, within Synpcc7942_0255 at 251.891 kb on - strand, within Synpcc7942_0255 at 251.891 kb on - strand, within Synpcc7942_0255 at 251.905 kb on - strand, within Synpcc7942_0255 at 252.008 kb on + strand, within Synpcc7942_0255 at 252.016 kb on - strand, within Synpcc7942_0255 at 252.016 kb on - strand, within Synpcc7942_0255 at 252.017 kb on + strand, within Synpcc7942_0255 at 252.050 kb on + strand, within Synpcc7942_0255 at 252.055 kb on + strand, within Synpcc7942_0255 at 252.058 kb on - strand, within Synpcc7942_0255 at 252.062 kb on + strand, within Synpcc7942_0255 at 252.062 kb on + strand, within Synpcc7942_0255 at 252.063 kb on - strand, within Synpcc7942_0255 at 252.175 kb on + strand, within Synpcc7942_0255 at 252.216 kb on + strand, within Synpcc7942_0255 at 252.216 kb on + strand, within Synpcc7942_0255 at 252.216 kb on + strand, within Synpcc7942_0255 at 252.223 kb on + strand, within Synpcc7942_0255 at 252.224 kb on - strand, within Synpcc7942_0255 at 252.224 kb on - strand, within Synpcc7942_0255 at 252.224 kb on - strand, within Synpcc7942_0255 at 252.224 kb on - strand, within Synpcc7942_0255 at 252.247 kb on + strand, within Synpcc7942_0255 at 252.254 kb on + strand, within Synpcc7942_0255 at 252.254 kb on + strand, within Synpcc7942_0255 at 252.254 kb on + strand, within Synpcc7942_0255 at 252.262 kb on - strand, within Synpcc7942_0255 at 252.262 kb on - strand, within Synpcc7942_0255 at 252.289 kb on - strand, within Synpcc7942_0255 at 252.296 kb on - strand, within Synpcc7942_0255 at 252.309 kb on - strand, within Synpcc7942_0255 at 252.311 kb on + strand, within Synpcc7942_0255 at 252.323 kb on + strand, within Synpcc7942_0255 at 252.372 kb on - strand, within Synpcc7942_0255 at 252.377 kb on - strand, within Synpcc7942_0255 at 252.377 kb on - strand, within Synpcc7942_0255 at 252.389 kb on + strand, within Synpcc7942_0255 at 252.409 kb on + strand, within Synpcc7942_0255 at 252.432 kb on + strand, within Synpcc7942_0255 at 252.443 kb on + strand, within Synpcc7942_0255 at 252.446 kb on - strand, within Synpcc7942_0255 at 252.463 kb on + strand, within Synpcc7942_0255 at 252.509 kb on - strand, within Synpcc7942_0255 at 252.556 kb on + strand, within Synpcc7942_0255 at 252.578 kb on - strand, within Synpcc7942_0255 at 252.578 kb on - strand, within Synpcc7942_0255 at 252.578 kb on - strand, within Synpcc7942_0255 at 252.610 kb on - strand, within Synpcc7942_0255 at 252.610 kb on - strand, within Synpcc7942_0255 at 252.623 kb on + strand, within Synpcc7942_0255 at 252.631 kb on - strand, within Synpcc7942_0255 at 252.633 kb on - strand at 252.633 kb on - strand, within Synpcc7942_0255 at 252.633 kb on - strand, within Synpcc7942_0255 at 252.634 kb on - strand, within Synpcc7942_0255 at 252.706 kb on + strand at 252.706 kb on + strand at 252.766 kb on + strand at 252.811 kb on + strand at 252.811 kb on + strand at 252.824 kb on - strand at 252.835 kb on + strand at 252.865 kb on + strand at 252.883 kb on + strand at 252.884 kb on - strand at 252.885 kb on - strand at 252.886 kb on - strand at 252.891 kb on - strand at 252.891 kb on - strand at 252.898 kb on + strand at 252.915 kb on + strand at 252.915 kb on + strand at 252.923 kb on - strand at 252.970 kb on + strand at 253.000 kb on + strand, within ama at 253.008 kb on - strand, within ama at 253.012 kb on + strand, within ama at 253.028 kb on - strand, within ama at 253.112 kb on + strand, within ama at 253.374 kb on + strand, within ama at 253.382 kb on - strand, within ama at 253.382 kb on - strand, within ama at 253.408 kb on + strand, within ama at 253.416 kb on - strand, within ama at 253.446 kb on + strand, within ama at 253.464 kb on - strand, within ama at 253.474 kb on - strand, within ama at 253.491 kb on - strand, within ama at 253.558 kb on + strand, within ama at 253.558 kb on - strand, within ama at 253.575 kb on - strand, within ama at 253.585 kb on + strand, within ama at 253.585 kb on + strand, within ama at 253.593 kb on - strand, within ama at 253.601 kb on + strand, within ama at 253.613 kb on + strand, within ama at 253.614 kb on - strand, within ama at 253.618 kb on + strand, within ama at 253.618 kb on - strand, within ama at 253.621 kb on - strand, within ama at 253.623 kb on - strand, within ama at 253.633 kb on - strand, within ama at 253.650 kb on + strand, within ama at 253.656 kb on + strand, within ama at 253.664 kb on - strand, within ama at 253.664 kb on - strand, within ama at 253.686 kb on + strand, within ama at 253.706 kb on - strand, within ama at 253.786 kb on + strand, within ama at 253.804 kb on - strand, within ama at 253.813 kb on + strand, within ama at 253.821 kb on - strand, within ama at 253.821 kb on - strand, within ama at 253.821 kb on - strand, within ama at 253.821 kb on - strand, within ama at 253.821 kb on - strand, within ama at 253.858 kb on - strand, within ama at 253.881 kb on - strand, within ama at 253.925 kb on + strand, within ama at 253.931 kb on - strand, within ama at 253.933 kb on - strand, within ama at 253.939 kb on - strand, within ama at 253.939 kb on - strand, within ama at 253.950 kb on - strand, within ama at 254.052 kb on - strand at 254.829 kb on + strand at 254.839 kb on + strand at 254.839 kb on + strand at 254.846 kb on + strand at 254.847 kb on - strand at 254.849 kb on - strand at 254.909 kb on + strand at 254.917 kb on - strand at 254.917 kb on - strand at 254.977 kb on - strand, within Synpcc7942_0258 at 255.044 kb on + strand, within Synpcc7942_0258 at 255.044 kb on + strand, within Synpcc7942_0258 at 255.044 kb on + strand, within Synpcc7942_0258 at 255.052 kb on - strand, within Synpcc7942_0258 at 255.052 kb on - strand, within Synpcc7942_0258 at 255.052 kb on - strand, within Synpcc7942_0258 at 255.052 kb on - strand, within Synpcc7942_0258 at 255.054 kb on - strand, within Synpcc7942_0258 at 255.067 kb on - strand, within Synpcc7942_0258 at 255.076 kb on - strand, within Synpcc7942_0258 at 255.078 kb on + strand, within Synpcc7942_0258
Per-strain Table
Position Strand Gene LocusTag Fraction Flask growth in 60 uE Light-Dark 12:12 remove 251,883 + Synpcc7942_0255 0.30 +0.4 251,891 - Synpcc7942_0255 0.30 -0.9 251,891 - Synpcc7942_0255 0.30 +0.4 251,905 - Synpcc7942_0255 0.31 +0.1 252,008 + Synpcc7942_0255 0.39 +0.1 252,016 - Synpcc7942_0255 0.40 +0.7 252,016 - Synpcc7942_0255 0.40 -0.7 252,017 + Synpcc7942_0255 0.40 -0.1 252,050 + Synpcc7942_0255 0.43 +1.0 252,055 + Synpcc7942_0255 0.43 +0.3 252,058 - Synpcc7942_0255 0.43 -0.5 252,062 + Synpcc7942_0255 0.44 +0.6 252,062 + Synpcc7942_0255 0.44 +0.1 252,063 - Synpcc7942_0255 0.44 -0.6 252,175 + Synpcc7942_0255 0.52 -0.6 252,216 + Synpcc7942_0255 0.56 -0.5 252,216 + Synpcc7942_0255 0.56 +0.5 252,216 + Synpcc7942_0255 0.56 -0.8 252,223 + Synpcc7942_0255 0.56 +0.1 252,224 - Synpcc7942_0255 0.56 -1.1 252,224 - Synpcc7942_0255 0.56 -0.0 252,224 - Synpcc7942_0255 0.56 +0.4 252,224 - Synpcc7942_0255 0.56 +0.9 252,247 + Synpcc7942_0255 0.58 -0.2 252,254 + Synpcc7942_0255 0.59 +0.7 252,254 + Synpcc7942_0255 0.59 -2.4 252,254 + Synpcc7942_0255 0.59 -2.5 252,262 - Synpcc7942_0255 0.59 +0.1 252,262 - Synpcc7942_0255 0.59 +0.7 252,289 - Synpcc7942_0255 0.61 +0.2 252,296 - Synpcc7942_0255 0.62 -0.7 252,309 - Synpcc7942_0255 0.63 -0.0 252,311 + Synpcc7942_0255 0.63 +0.3 252,323 + Synpcc7942_0255 0.64 +0.2 252,372 - Synpcc7942_0255 0.68 -0.2 252,377 - Synpcc7942_0255 0.68 +0.9 252,377 - Synpcc7942_0255 0.68 +0.3 252,389 + Synpcc7942_0255 0.69 -1.4 252,409 + Synpcc7942_0255 0.71 +0.0 252,432 + Synpcc7942_0255 0.73 +0.4 252,443 + Synpcc7942_0255 0.73 -0.3 252,446 - Synpcc7942_0255 0.74 +0.4 252,463 + Synpcc7942_0255 0.75 +0.7 252,509 - Synpcc7942_0255 0.79 -1.3 252,556 + Synpcc7942_0255 0.82 +0.1 252,578 - Synpcc7942_0255 0.84 +0.5 252,578 - Synpcc7942_0255 0.84 +0.2 252,578 - Synpcc7942_0255 0.84 +0.3 252,610 - Synpcc7942_0255 0.86 +0.5 252,610 - Synpcc7942_0255 0.86 -0.4 252,623 + Synpcc7942_0255 0.87 -0.3 252,631 - Synpcc7942_0255 0.88 -0.4 252,633 - -1.0 252,633 - Synpcc7942_0255 0.88 +0.1 252,633 - Synpcc7942_0255 0.88 +0.3 252,634 - Synpcc7942_0255 0.88 +1.2 252,706 + -0.6 252,706 + +2.2 252,766 + -0.9 252,811 + -0.5 252,811 + -1.2 252,824 - -1.3 252,835 + -1.2 252,865 + -1.0 252,883 + +1.0 252,884 - -0.3 252,885 - +0.7 252,886 - +3.3 252,891 - +0.9 252,891 - -0.3 252,898 + +0.8 252,915 + +0.9 252,915 + +0.6 252,923 - -0.4 252,970 + -0.8 253,000 + ama Synpcc7942_0256 0.10 -0.4 253,008 - ama Synpcc7942_0256 0.11 +1.5 253,012 + ama Synpcc7942_0256 0.11 +2.7 253,028 - ama Synpcc7942_0256 0.13 +1.3 253,112 + ama Synpcc7942_0256 0.19 +1.0 253,374 + ama Synpcc7942_0256 0.41 +1.1 253,382 - ama Synpcc7942_0256 0.41 +0.6 253,382 - ama Synpcc7942_0256 0.41 +1.2 253,408 + ama Synpcc7942_0256 0.44 +0.8 253,416 - ama Synpcc7942_0256 0.44 +1.2 253,446 + ama Synpcc7942_0256 0.47 +1.7 253,464 - ama Synpcc7942_0256 0.48 +0.8 253,474 - ama Synpcc7942_0256 0.49 +0.7 253,491 - ama Synpcc7942_0256 0.50 +1.2 253,558 + ama Synpcc7942_0256 0.56 +0.6 253,558 - ama Synpcc7942_0256 0.56 +0.7 253,575 - ama Synpcc7942_0256 0.57 -2.6 253,585 + ama Synpcc7942_0256 0.58 +0.6 253,585 + ama Synpcc7942_0256 0.58 +0.9 253,593 - ama Synpcc7942_0256 0.59 +1.7 253,601 + ama Synpcc7942_0256 0.59 +0.9 253,613 + ama Synpcc7942_0256 0.60 +0.2 253,614 - ama Synpcc7942_0256 0.60 +2.0 253,618 + ama Synpcc7942_0256 0.61 +1.5 253,618 - ama Synpcc7942_0256 0.61 +1.7 253,621 - ama Synpcc7942_0256 0.61 -0.5 253,623 - ama Synpcc7942_0256 0.61 +1.7 253,633 - ama Synpcc7942_0256 0.62 +1.3 253,650 + ama Synpcc7942_0256 0.63 +1.8 253,656 + ama Synpcc7942_0256 0.64 +1.8 253,664 - ama Synpcc7942_0256 0.64 +2.8 253,664 - ama Synpcc7942_0256 0.64 +1.5 253,686 + ama Synpcc7942_0256 0.66 +1.5 253,706 - ama Synpcc7942_0256 0.68 +1.2 253,786 + ama Synpcc7942_0256 0.74 +1.0 253,804 - ama Synpcc7942_0256 0.76 +1.3 253,813 + ama Synpcc7942_0256 0.77 +1.5 253,821 - ama Synpcc7942_0256 0.77 +0.5 253,821 - ama Synpcc7942_0256 0.77 -0.1 253,821 - ama Synpcc7942_0256 0.77 -0.3 253,821 - ama Synpcc7942_0256 0.77 +0.7 253,821 - ama Synpcc7942_0256 0.77 -1.8 253,858 - ama Synpcc7942_0256 0.80 +2.3 253,881 - ama Synpcc7942_0256 0.82 +1.2 253,925 + ama Synpcc7942_0256 0.86 +0.2 253,931 - ama Synpcc7942_0256 0.86 +0.8 253,933 - ama Synpcc7942_0256 0.86 +0.0 253,939 - ama Synpcc7942_0256 0.87 +0.7 253,939 - ama Synpcc7942_0256 0.87 +0.9 253,950 - ama Synpcc7942_0256 0.88 +1.7 254,052 - +1.1 254,829 + -1.4 254,839 + +0.0 254,839 + +0.0 254,846 + +0.5 254,847 - -0.3 254,849 - -0.9 254,909 + -0.1 254,917 - -1.0 254,917 - +0.1 254,977 - Synpcc7942_0258 0.19 -2.4 255,044 + Synpcc7942_0258 0.34 -0.2 255,044 + Synpcc7942_0258 0.34 -2.5 255,044 + Synpcc7942_0258 0.34 +0.9 255,052 - Synpcc7942_0258 0.36 -0.9 255,052 - Synpcc7942_0258 0.36 -0.2 255,052 - Synpcc7942_0258 0.36 -2.3 255,052 - Synpcc7942_0258 0.36 +0.2 255,054 - Synpcc7942_0258 0.36 -1.0 255,067 - Synpcc7942_0258 0.39 +0.4 255,076 - Synpcc7942_0258 0.41 -0.3 255,078 + Synpcc7942_0258 0.42 -0.5
Or see this region's nucleotide sequence