Experiment: Flask growth in 60 uE Light-Dark 12:12
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ggt and Synpcc7942_0186 are separated by 13 nucleotides Synpcc7942_0186 and Synpcc7942_0187 are separated by 6 nucleotides Synpcc7942_0187 and Synpcc7942_0188 are separated by 220 nucleotides
Synpcc7942_0185: ggt - gamma-glutamyltransferase. Threonine peptidase. MEROPS family T03, at 182,620 to 184,146
ggt
Synpcc7942_0186: Synpcc7942_0186 - possible DNA polymerase, at 184,160 to 184,411
_0186
Synpcc7942_0187: Synpcc7942_0187 - hypothetical protein, at 184,418 to 185,461
_0187
Synpcc7942_0188: Synpcc7942_0188 - diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), at 185,682 to 187,418
_0188
Position (kb) 184
185
186 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 183.420 kb on + strand, within ggt at 183.437 kb on + strand, within ggt at 183.439 kb on + strand, within ggt at 183.440 kb on - strand, within ggt at 183.445 kb on - strand, within ggt at 183.445 kb on - strand, within ggt at 183.458 kb on + strand, within ggt at 183.466 kb on - strand, within ggt at 183.471 kb on - strand, within ggt at 183.480 kb on + strand, within ggt at 183.488 kb on - strand, within ggt at 183.488 kb on - strand, within ggt at 183.517 kb on + strand, within ggt at 183.533 kb on - strand, within ggt at 183.552 kb on - strand, within ggt at 183.592 kb on + strand, within ggt at 183.605 kb on - strand, within ggt at 183.607 kb on + strand, within ggt at 183.612 kb on + strand, within ggt at 183.612 kb on + strand, within ggt at 183.625 kb on - strand at 183.646 kb on + strand, within ggt at 183.649 kb on - strand, within ggt at 183.655 kb on + strand, within ggt at 183.659 kb on - strand, within ggt at 183.672 kb on - strand, within ggt at 183.693 kb on + strand, within ggt at 183.703 kb on + strand, within ggt at 183.703 kb on + strand, within ggt at 183.703 kb on + strand, within ggt at 183.711 kb on - strand, within ggt at 183.728 kb on - strand, within ggt at 183.781 kb on - strand, within ggt at 183.791 kb on + strand, within ggt at 183.801 kb on + strand, within ggt at 183.868 kb on + strand, within ggt at 183.872 kb on + strand, within ggt at 183.879 kb on - strand, within ggt at 183.898 kb on + strand, within ggt at 183.905 kb on + strand, within ggt at 183.905 kb on + strand, within ggt at 183.953 kb on + strand, within ggt at 183.966 kb on - strand, within ggt at 183.966 kb on - strand, within ggt at 183.966 kb on - strand, within ggt at 183.970 kb on + strand, within ggt at 183.976 kb on + strand, within ggt at 183.976 kb on + strand, within ggt at 183.976 kb on + strand, within ggt at 183.984 kb on - strand, within ggt at 183.984 kb on - strand, within ggt at 183.993 kb on + strand, within ggt at 184.001 kb on - strand at 184.006 kb on + strand at 184.006 kb on + strand at 184.014 kb on - strand at 184.029 kb on + strand at 184.029 kb on + strand at 184.029 kb on + strand at 184.029 kb on + strand at 184.029 kb on + strand at 184.029 kb on + strand at 184.029 kb on + strand at 184.029 kb on + strand at 184.037 kb on - strand at 184.039 kb on + strand at 184.039 kb on + strand at 184.045 kb on + strand at 184.070 kb on + strand at 184.103 kb on - strand at 184.127 kb on + strand at 184.129 kb on + strand at 184.152 kb on - strand at 184.159 kb on - strand at 184.170 kb on + strand at 184.170 kb on - strand at 184.173 kb on - strand at 184.177 kb on + strand at 184.178 kb on - strand at 184.183 kb on - strand at 184.220 kb on - strand, within Synpcc7942_0186 at 184.292 kb on + strand, within Synpcc7942_0186 at 184.329 kb on + strand, within Synpcc7942_0186 at 184.342 kb on - strand, within Synpcc7942_0186 at 184.343 kb on + strand, within Synpcc7942_0186 at 184.343 kb on + strand at 184.343 kb on + strand, within Synpcc7942_0186 at 184.343 kb on + strand, within Synpcc7942_0186 at 184.343 kb on + strand, within Synpcc7942_0186 at 184.343 kb on + strand, within Synpcc7942_0186 at 184.351 kb on + strand, within Synpcc7942_0186 at 184.351 kb on - strand, within Synpcc7942_0186 at 184.351 kb on - strand, within Synpcc7942_0186 at 184.351 kb on - strand, within Synpcc7942_0186 at 184.351 kb on - strand, within Synpcc7942_0186 at 184.351 kb on - strand, within Synpcc7942_0186 at 184.351 kb on - strand at 184.351 kb on - strand, within Synpcc7942_0186 at 184.377 kb on + strand, within Synpcc7942_0186 at 184.386 kb on + strand at 184.387 kb on + strand at 184.395 kb on - strand at 184.395 kb on - strand at 184.421 kb on + strand at 184.423 kb on - strand at 184.455 kb on - strand at 184.467 kb on - strand at 184.493 kb on + strand at 184.527 kb on + strand, within Synpcc7942_0187 at 184.873 kb on + strand, within Synpcc7942_0187 at 184.905 kb on - strand, within Synpcc7942_0187 at 184.944 kb on + strand, within Synpcc7942_0187 at 185.076 kb on - strand, within Synpcc7942_0187 at 185.078 kb on + strand, within Synpcc7942_0187 at 185.275 kb on - strand, within Synpcc7942_0187 at 185.283 kb on - strand, within Synpcc7942_0187 at 185.379 kb on + strand at 185.428 kb on - strand at 185.453 kb on + strand at 185.472 kb on + strand at 185.480 kb on - strand at 185.516 kb on - strand at 185.541 kb on - strand at 185.567 kb on - strand at 185.567 kb on - strand at 185.572 kb on + strand at 185.588 kb on + strand at 185.588 kb on + strand at 185.601 kb on - strand at 185.645 kb on + strand at 185.645 kb on + strand at 185.653 kb on - strand at 185.671 kb on - strand at 185.747 kb on - strand at 185.779 kb on + strand at 185.779 kb on - strand at 185.787 kb on - strand at 185.790 kb on + strand at 185.792 kb on - strand at 185.792 kb on - strand at 185.797 kb on - strand at 185.798 kb on - strand at 185.798 kb on - strand at 185.826 kb on + strand at 185.859 kb on - strand, within Synpcc7942_0188 at 185.865 kb on + strand, within Synpcc7942_0188 at 185.920 kb on + strand, within Synpcc7942_0188 at 185.923 kb on + strand, within Synpcc7942_0188 at 185.938 kb on - strand, within Synpcc7942_0188 at 185.967 kb on + strand, within Synpcc7942_0188 at 185.967 kb on + strand, within Synpcc7942_0188 at 185.975 kb on - strand, within Synpcc7942_0188 at 185.975 kb on - strand, within Synpcc7942_0188 at 185.975 kb on - strand, within Synpcc7942_0188 at 185.975 kb on - strand, within Synpcc7942_0188 at 185.990 kb on - strand, within Synpcc7942_0188 at 186.037 kb on - strand, within Synpcc7942_0188 at 186.045 kb on + strand, within Synpcc7942_0188 at 186.059 kb on + strand, within Synpcc7942_0188 at 186.059 kb on + strand, within Synpcc7942_0188 at 186.059 kb on + strand, within Synpcc7942_0188 at 186.067 kb on - strand, within Synpcc7942_0188 at 186.068 kb on - strand, within Synpcc7942_0188 at 186.074 kb on - strand, within Synpcc7942_0188 at 186.102 kb on + strand, within Synpcc7942_0188 at 186.152 kb on + strand, within Synpcc7942_0188 at 186.153 kb on + strand, within Synpcc7942_0188 at 186.161 kb on - strand, within Synpcc7942_0188 at 186.167 kb on - strand, within Synpcc7942_0188 at 186.172 kb on - strand, within Synpcc7942_0188 at 186.206 kb on + strand, within Synpcc7942_0188 at 186.218 kb on + strand, within Synpcc7942_0188 at 186.230 kb on + strand, within Synpcc7942_0188 at 186.236 kb on - strand, within Synpcc7942_0188 at 186.237 kb on + strand, within Synpcc7942_0188 at 186.250 kb on - strand, within Synpcc7942_0188 at 186.272 kb on + strand, within Synpcc7942_0188 at 186.272 kb on + strand, within Synpcc7942_0188 at 186.298 kb on - strand, within Synpcc7942_0188 at 186.313 kb on + strand, within Synpcc7942_0188 at 186.364 kb on + strand, within Synpcc7942_0188 at 186.371 kb on - strand, within Synpcc7942_0188 at 186.374 kb on + strand, within Synpcc7942_0188 at 186.382 kb on - strand, within Synpcc7942_0188 at 186.409 kb on + strand, within Synpcc7942_0188 at 186.417 kb on + strand, within Synpcc7942_0188 at 186.417 kb on + strand, within Synpcc7942_0188 at 186.425 kb on - strand, within Synpcc7942_0188 at 186.427 kb on + strand, within Synpcc7942_0188 at 186.427 kb on + strand, within Synpcc7942_0188 at 186.434 kb on + strand, within Synpcc7942_0188 at 186.435 kb on - strand, within Synpcc7942_0188 at 186.450 kb on + strand, within Synpcc7942_0188 at 186.450 kb on + strand, within Synpcc7942_0188 at 186.450 kb on + strand, within Synpcc7942_0188
Per-strain Table
Position Strand Gene LocusTag Fraction Flask growth in 60 uE Light-Dark 12:12 remove 183,420 + ggt Synpcc7942_0185 0.52 +0.2 183,437 + ggt Synpcc7942_0185 0.54 -0.8 183,439 + ggt Synpcc7942_0185 0.54 +1.6 183,440 - ggt Synpcc7942_0185 0.54 +0.5 183,445 - ggt Synpcc7942_0185 0.54 +1.6 183,445 - ggt Synpcc7942_0185 0.54 +1.6 183,458 + ggt Synpcc7942_0185 0.55 -0.3 183,466 - ggt Synpcc7942_0185 0.55 +1.3 183,471 - ggt Synpcc7942_0185 0.56 +0.3 183,480 + ggt Synpcc7942_0185 0.56 +1.7 183,488 - ggt Synpcc7942_0185 0.57 +1.1 183,488 - ggt Synpcc7942_0185 0.57 -0.9 183,517 + ggt Synpcc7942_0185 0.59 +1.8 183,533 - ggt Synpcc7942_0185 0.60 +1.8 183,552 - ggt Synpcc7942_0185 0.61 +1.7 183,592 + ggt Synpcc7942_0185 0.64 +1.5 183,605 - ggt Synpcc7942_0185 0.65 +1.2 183,607 + ggt Synpcc7942_0185 0.65 -0.2 183,612 + ggt Synpcc7942_0185 0.65 +1.7 183,612 + ggt Synpcc7942_0185 0.65 +1.7 183,625 - +2.3 183,646 + ggt Synpcc7942_0185 0.67 +1.4 183,649 - ggt Synpcc7942_0185 0.67 +0.8 183,655 + ggt Synpcc7942_0185 0.68 +1.4 183,659 - ggt Synpcc7942_0185 0.68 +1.2 183,672 - ggt Synpcc7942_0185 0.69 +1.1 183,693 + ggt Synpcc7942_0185 0.70 +1.6 183,703 + ggt Synpcc7942_0185 0.71 +0.2 183,703 + ggt Synpcc7942_0185 0.71 +0.7 183,703 + ggt Synpcc7942_0185 0.71 +2.4 183,711 - ggt Synpcc7942_0185 0.71 +1.7 183,728 - ggt Synpcc7942_0185 0.73 +1.4 183,781 - ggt Synpcc7942_0185 0.76 +0.8 183,791 + ggt Synpcc7942_0185 0.77 +1.2 183,801 + ggt Synpcc7942_0185 0.77 +1.2 183,868 + ggt Synpcc7942_0185 0.82 +0.2 183,872 + ggt Synpcc7942_0185 0.82 +1.2 183,879 - ggt Synpcc7942_0185 0.82 -0.2 183,898 + ggt Synpcc7942_0185 0.84 -0.6 183,905 + ggt Synpcc7942_0185 0.84 +1.4 183,905 + ggt Synpcc7942_0185 0.84 -2.2 183,953 + ggt Synpcc7942_0185 0.87 +1.7 183,966 - ggt Synpcc7942_0185 0.88 +1.1 183,966 - ggt Synpcc7942_0185 0.88 +1.1 183,966 - ggt Synpcc7942_0185 0.88 +1.4 183,970 + ggt Synpcc7942_0185 0.88 +1.3 183,976 + ggt Synpcc7942_0185 0.89 +0.9 183,976 + ggt Synpcc7942_0185 0.89 +0.4 183,976 + ggt Synpcc7942_0185 0.89 -0.6 183,984 - ggt Synpcc7942_0185 0.89 +2.3 183,984 - ggt Synpcc7942_0185 0.89 +1.4 183,993 + ggt Synpcc7942_0185 0.90 +1.6 184,001 - +1.2 184,006 + +1.5 184,006 + +0.4 184,014 - +0.5 184,029 + +2.0 184,029 + +2.2 184,029 + +1.6 184,029 + +1.7 184,029 + +0.1 184,029 + +0.3 184,029 + +1.4 184,029 + +2.1 184,037 - +0.6 184,039 + +0.2 184,039 + +1.1 184,045 + +1.8 184,070 + +0.3 184,103 - +4.3 184,127 + +0.9 184,129 + +1.3 184,152 - +0.3 184,159 - -1.4 184,170 + +1.1 184,170 - -0.3 184,173 - +0.4 184,177 + +0.1 184,178 - +0.2 184,183 - +1.0 184,220 - Synpcc7942_0186 0.24 -0.2 184,292 + Synpcc7942_0186 0.52 +0.4 184,329 + Synpcc7942_0186 0.67 -0.6 184,342 - Synpcc7942_0186 0.72 -0.5 184,343 + Synpcc7942_0186 0.73 +0.3 184,343 + +1.0 184,343 + Synpcc7942_0186 0.73 +0.2 184,343 + Synpcc7942_0186 0.73 +0.8 184,343 + Synpcc7942_0186 0.73 -0.8 184,343 + Synpcc7942_0186 0.73 +0.5 184,351 + Synpcc7942_0186 0.76 +0.8 184,351 - Synpcc7942_0186 0.76 -0.8 184,351 - Synpcc7942_0186 0.76 +0.7 184,351 - Synpcc7942_0186 0.76 +0.2 184,351 - Synpcc7942_0186 0.76 +0.9 184,351 - Synpcc7942_0186 0.76 -0.5 184,351 - +0.6 184,351 - Synpcc7942_0186 0.76 +0.3 184,377 + Synpcc7942_0186 0.86 +1.1 184,386 + +0.7 184,387 + +0.4 184,395 - -0.5 184,395 - -0.8 184,421 + -1.0 184,423 - +0.4 184,455 - +0.3 184,467 - +0.7 184,493 + +0.3 184,527 + Synpcc7942_0187 0.10 -3.1 184,873 + Synpcc7942_0187 0.44 -2.7 184,905 - Synpcc7942_0187 0.47 -3.1 184,944 + Synpcc7942_0187 0.50 -4.0 185,076 - Synpcc7942_0187 0.63 -0.6 185,078 + Synpcc7942_0187 0.63 -3.2 185,275 - Synpcc7942_0187 0.82 -2.0 185,283 - Synpcc7942_0187 0.83 -2.8 185,379 + -0.8 185,428 - -3.3 185,453 + -2.5 185,472 + -0.5 185,480 - -0.5 185,516 - +0.0 185,541 - +0.0 185,567 - +0.2 185,567 - -0.6 185,572 + -1.8 185,588 + -1.0 185,588 + -2.9 185,601 - -0.4 185,645 + +1.7 185,645 + -0.4 185,653 - +0.6 185,671 - -1.2 185,747 - +1.3 185,779 + +2.0 185,779 - +0.4 185,787 - -0.5 185,790 + +0.4 185,792 - +0.1 185,792 - -0.1 185,797 - +1.0 185,798 - -0.7 185,798 - +0.5 185,826 + +2.0 185,859 - Synpcc7942_0188 0.10 -0.2 185,865 + Synpcc7942_0188 0.11 +0.3 185,920 + Synpcc7942_0188 0.14 -0.3 185,923 + Synpcc7942_0188 0.14 +0.5 185,938 - Synpcc7942_0188 0.15 +0.1 185,967 + Synpcc7942_0188 0.16 +1.8 185,967 + Synpcc7942_0188 0.16 -0.9 185,975 - Synpcc7942_0188 0.17 -0.5 185,975 - Synpcc7942_0188 0.17 +0.3 185,975 - Synpcc7942_0188 0.17 +0.7 185,975 - Synpcc7942_0188 0.17 +0.3 185,990 - Synpcc7942_0188 0.18 -0.1 186,037 - Synpcc7942_0188 0.20 +0.3 186,045 + Synpcc7942_0188 0.21 +0.2 186,059 + Synpcc7942_0188 0.22 +0.3 186,059 + Synpcc7942_0188 0.22 -0.6 186,059 + Synpcc7942_0188 0.22 +2.0 186,067 - Synpcc7942_0188 0.22 -0.1 186,068 - Synpcc7942_0188 0.22 -2.6 186,074 - Synpcc7942_0188 0.23 +0.2 186,102 + Synpcc7942_0188 0.24 +0.4 186,152 + Synpcc7942_0188 0.27 +0.1 186,153 + Synpcc7942_0188 0.27 -1.1 186,161 - Synpcc7942_0188 0.28 -1.6 186,167 - Synpcc7942_0188 0.28 -1.0 186,172 - Synpcc7942_0188 0.28 +0.9 186,206 + Synpcc7942_0188 0.30 -1.5 186,218 + Synpcc7942_0188 0.31 -0.1 186,230 + Synpcc7942_0188 0.32 -0.2 186,236 - Synpcc7942_0188 0.32 +2.2 186,237 + Synpcc7942_0188 0.32 +0.8 186,250 - Synpcc7942_0188 0.33 +0.7 186,272 + Synpcc7942_0188 0.34 -1.1 186,272 + Synpcc7942_0188 0.34 -0.1 186,298 - Synpcc7942_0188 0.35 -0.5 186,313 + Synpcc7942_0188 0.36 -1.4 186,364 + Synpcc7942_0188 0.39 +0.4 186,371 - Synpcc7942_0188 0.40 -0.9 186,374 + Synpcc7942_0188 0.40 -0.4 186,382 - Synpcc7942_0188 0.40 -0.7 186,409 + Synpcc7942_0188 0.42 +0.0 186,417 + Synpcc7942_0188 0.42 +1.2 186,417 + Synpcc7942_0188 0.42 -0.5 186,425 - Synpcc7942_0188 0.43 +0.2 186,427 + Synpcc7942_0188 0.43 -0.7 186,427 + Synpcc7942_0188 0.43 +0.3 186,434 + Synpcc7942_0188 0.43 -0.6 186,435 - Synpcc7942_0188 0.43 +1.5 186,450 + Synpcc7942_0188 0.44 +0.2 186,450 + Synpcc7942_0188 0.44 -0.8 186,450 + Synpcc7942_0188 0.44 +0.1
Or see this region's nucleotide sequence