Experiment: Flask growth in 60 uE Light-Dark 12:12
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nadE and nadD overlap by 4 nucleotides nadD and Synpcc7942_0107 are separated by 12 nucleotides Synpcc7942_0107 and Synpcc7942_0108 are separated by 172 nucleotides Synpcc7942_0108 and Synpcc7942_0109 are separated by 60 nucleotides
Synpcc7942_0105: nadE - NAD synthetase, at 104,653 to 106,377
nadE
Synpcc7942_0106: nadD - nicotinic acid mononucleotide adenyltransferase, at 106,374 to 106,952
nadD
Synpcc7942_0107: Synpcc7942_0107 - Small GTP-binding protein domain, at 106,965 to 108,383
_0107
Synpcc7942_0108: Synpcc7942_0108 - sulfiredoxin, at 108,556 to 108,816
_0108
Synpcc7942_0109: Synpcc7942_0109 - DNA-binding ferritin-like protein (oxidative damage protectant)-like, at 108,877 to 109,383
_0109
Position (kb)
106
107
108
109 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 106.379 kb on - strand at 106.396 kb on + strand at 106.396 kb on + strand at 106.396 kb on + strand at 106.404 kb on - strand at 106.410 kb on - strand at 106.763 kb on - strand, within nadD at 106.763 kb on - strand, within nadD at 106.801 kb on - strand, within nadD at 106.829 kb on + strand, within nadD at 106.837 kb on - strand, within nadD at 106.892 kb on - strand, within nadD at 106.935 kb on + strand at 106.954 kb on + strand at 106.955 kb on - strand at 107.004 kb on + strand at 107.030 kb on + strand at 107.038 kb on - strand at 107.040 kb on + strand at 107.079 kb on + strand at 108.344 kb on - strand at 108.344 kb on - strand at 108.346 kb on + strand at 108.346 kb on + strand at 108.365 kb on + strand at 108.408 kb on + strand at 108.411 kb on - strand at 108.411 kb on - strand at 108.436 kb on + strand at 108.436 kb on + strand at 108.436 kb on + strand at 108.441 kb on + strand at 108.444 kb on - strand at 108.444 kb on - strand at 108.444 kb on - strand at 108.444 kb on - strand at 108.444 kb on - strand at 108.444 kb on - strand at 108.444 kb on - strand at 108.449 kb on - strand at 108.449 kb on - strand at 108.452 kb on + strand at 108.452 kb on + strand at 108.460 kb on - strand at 108.460 kb on - strand at 108.462 kb on - strand at 108.463 kb on - strand at 108.463 kb on - strand at 108.465 kb on + strand at 108.473 kb on - strand at 108.473 kb on - strand at 108.473 kb on - strand at 108.571 kb on - strand at 108.571 kb on - strand at 108.573 kb on - strand at 108.578 kb on - strand at 108.603 kb on + strand, within Synpcc7942_0108 at 108.603 kb on - strand, within Synpcc7942_0108 at 108.608 kb on + strand, within Synpcc7942_0108 at 108.611 kb on - strand, within Synpcc7942_0108 at 108.625 kb on + strand, within Synpcc7942_0108 at 108.625 kb on + strand, within Synpcc7942_0108 at 108.638 kb on - strand, within Synpcc7942_0108 at 108.650 kb on + strand, within Synpcc7942_0108 at 108.665 kb on - strand, within Synpcc7942_0108 at 108.672 kb on + strand, within Synpcc7942_0108 at 108.750 kb on - strand, within Synpcc7942_0108 at 108.793 kb on + strand at 108.793 kb on + strand at 108.801 kb on - strand at 108.801 kb on - strand at 108.810 kb on + strand at 108.829 kb on + strand at 108.829 kb on + strand at 108.837 kb on - strand at 108.837 kb on - strand at 108.837 kb on - strand at 108.837 kb on - strand at 108.842 kb on + strand at 108.844 kb on + strand at 108.860 kb on - strand at 108.873 kb on - strand at 108.933 kb on + strand, within Synpcc7942_0109 at 108.953 kb on + strand, within Synpcc7942_0109 at 108.978 kb on + strand, within Synpcc7942_0109 at 108.983 kb on + strand, within Synpcc7942_0109 at 108.987 kb on + strand, within Synpcc7942_0109 at 108.989 kb on + strand, within Synpcc7942_0109 at 108.989 kb on - strand, within Synpcc7942_0109 at 108.995 kb on - strand, within Synpcc7942_0109 at 108.997 kb on - strand, within Synpcc7942_0109 at 109.044 kb on + strand, within Synpcc7942_0109 at 109.045 kb on - strand, within Synpcc7942_0109 at 109.050 kb on - strand, within Synpcc7942_0109 at 109.052 kb on - strand, within Synpcc7942_0109 at 109.059 kb on - strand, within Synpcc7942_0109 at 109.089 kb on + strand, within Synpcc7942_0109 at 109.152 kb on - strand, within Synpcc7942_0109 at 109.158 kb on - strand, within Synpcc7942_0109 at 109.227 kb on - strand at 109.227 kb on - strand, within Synpcc7942_0109 at 109.295 kb on + strand, within Synpcc7942_0109 at 109.303 kb on - strand, within Synpcc7942_0109 at 109.314 kb on + strand, within Synpcc7942_0109 at 109.359 kb on + strand at 109.362 kb on + strand at 109.370 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Flask growth in 60 uE Light-Dark 12:12 remove 106,379 - +0.2 106,396 + +1.4 106,396 + +2.0 106,396 + +1.0 106,404 - +0.0 106,410 - -0.9 106,763 - nadD Synpcc7942_0106 0.67 +0.6 106,763 - nadD Synpcc7942_0106 0.67 +1.0 106,801 - nadD Synpcc7942_0106 0.74 -0.5 106,829 + nadD Synpcc7942_0106 0.79 +0.2 106,837 - nadD Synpcc7942_0106 0.80 -0.4 106,892 - nadD Synpcc7942_0106 0.89 +0.2 106,935 + +1.5 106,954 + +0.1 106,955 - -2.7 107,004 + -1.5 107,030 + -0.1 107,038 - -1.2 107,040 + -1.6 107,079 + -4.1 108,344 - +0.6 108,344 - -1.4 108,346 + -1.5 108,346 + -0.8 108,365 + -0.8 108,408 + -0.3 108,411 - -0.6 108,411 - -1.7 108,436 + +0.4 108,436 + +0.1 108,436 + -0.9 108,441 + +0.7 108,444 - +1.2 108,444 - -1.2 108,444 - -4.8 108,444 - -0.0 108,444 - -0.5 108,444 - -0.6 108,444 - +0.1 108,449 - +0.2 108,449 - -2.0 108,452 + +0.6 108,452 + +0.4 108,460 - +0.4 108,460 - -0.7 108,462 - -0.3 108,463 - -1.1 108,463 - +1.0 108,465 + -0.1 108,473 - +0.1 108,473 - -0.3 108,473 - -2.3 108,571 - +0.1 108,571 - +0.5 108,573 - +0.1 108,578 - +0.1 108,603 + Synpcc7942_0108 0.18 +0.2 108,603 - Synpcc7942_0108 0.18 -0.3 108,608 + Synpcc7942_0108 0.20 -0.3 108,611 - Synpcc7942_0108 0.21 +0.4 108,625 + Synpcc7942_0108 0.26 -1.9 108,625 + Synpcc7942_0108 0.26 +0.4 108,638 - Synpcc7942_0108 0.31 -0.6 108,650 + Synpcc7942_0108 0.36 +0.3 108,665 - Synpcc7942_0108 0.42 +1.1 108,672 + Synpcc7942_0108 0.44 +2.0 108,750 - Synpcc7942_0108 0.74 -0.2 108,793 + -0.3 108,793 + -0.1 108,801 - +0.1 108,801 - +0.0 108,810 + -1.4 108,829 + -1.8 108,829 + -0.8 108,837 - -0.4 108,837 - +0.0 108,837 - -0.3 108,837 - -0.6 108,842 + -0.0 108,844 + +1.0 108,860 - +0.4 108,873 - -2.0 108,933 + Synpcc7942_0109 0.11 -0.3 108,953 + Synpcc7942_0109 0.15 -0.9 108,978 + Synpcc7942_0109 0.20 -0.0 108,983 + Synpcc7942_0109 0.21 -1.6 108,987 + Synpcc7942_0109 0.22 +0.2 108,989 + Synpcc7942_0109 0.22 -1.6 108,989 - Synpcc7942_0109 0.22 -0.4 108,995 - Synpcc7942_0109 0.23 +1.0 108,997 - Synpcc7942_0109 0.24 -0.9 109,044 + Synpcc7942_0109 0.33 -1.2 109,045 - Synpcc7942_0109 0.33 -1.3 109,050 - Synpcc7942_0109 0.34 -0.0 109,052 - Synpcc7942_0109 0.35 -0.6 109,059 - Synpcc7942_0109 0.36 -0.3 109,089 + Synpcc7942_0109 0.42 -0.8 109,152 - Synpcc7942_0109 0.54 -0.7 109,158 - Synpcc7942_0109 0.55 -1.2 109,227 - -1.2 109,227 - Synpcc7942_0109 0.69 -1.0 109,295 + Synpcc7942_0109 0.82 -0.5 109,303 - Synpcc7942_0109 0.84 +2.3 109,314 + Synpcc7942_0109 0.86 -0.6 109,359 + -0.2 109,362 + -1.3 109,370 - -0.0
Or see this region's nucleotide sequence