Experiment: Glycerol (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2021 and Pf6N2E2_2022 are separated by 68 nucleotides Pf6N2E2_2022 and Pf6N2E2_2023 are separated by 184 nucleotides Pf6N2E2_2023 and Pf6N2E2_2024 are separated by 7 nucleotides
Pf6N2E2_2021 - Excinuclease ABC subunit C, at 2,409,570 to 2,411,327
_2021
Pf6N2E2_2022 - BarA-associated response regulator UvrY (= GacA = SirA), at 2,411,396 to 2,411,980
_2022
Pf6N2E2_2023 - Protein export cytoplasm protein SecA ATPase RNA helicase (TC 3.A.5.1.1), at 2,412,165 to 2,412,599
_2023
Pf6N2E2_2024 - Hydrolase, carbon-nitrogen family, at 2,412,607 to 2,413,368
_2024
Position (kb)
2411
2412 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2410.486 kb on + strand, within Pf6N2E2_2021 at 2410.486 kb on + strand, within Pf6N2E2_2021 at 2410.487 kb on - strand, within Pf6N2E2_2021 at 2410.487 kb on - strand, within Pf6N2E2_2021 at 2410.487 kb on - strand, within Pf6N2E2_2021 at 2410.489 kb on + strand, within Pf6N2E2_2021 at 2410.835 kb on - strand, within Pf6N2E2_2021 at 2410.889 kb on - strand, within Pf6N2E2_2021 at 2410.918 kb on + strand, within Pf6N2E2_2021 at 2410.918 kb on + strand, within Pf6N2E2_2021 at 2410.919 kb on - strand, within Pf6N2E2_2021 at 2410.921 kb on + strand, within Pf6N2E2_2021 at 2410.921 kb on + strand, within Pf6N2E2_2021 at 2410.921 kb on + strand, within Pf6N2E2_2021 at 2410.922 kb on - strand, within Pf6N2E2_2021 at 2410.922 kb on - strand, within Pf6N2E2_2021 at 2411.011 kb on + strand, within Pf6N2E2_2021 at 2411.011 kb on + strand, within Pf6N2E2_2021 at 2411.083 kb on + strand, within Pf6N2E2_2021 at 2411.090 kb on - strand, within Pf6N2E2_2021 at 2411.119 kb on + strand, within Pf6N2E2_2021 at 2411.119 kb on + strand, within Pf6N2E2_2021 at 2411.257 kb on + strand at 2411.258 kb on - strand at 2411.299 kb on + strand at 2411.300 kb on - strand at 2411.480 kb on - strand, within Pf6N2E2_2022 at 2411.490 kb on + strand, within Pf6N2E2_2022 at 2411.490 kb on + strand, within Pf6N2E2_2022 at 2411.491 kb on - strand, within Pf6N2E2_2022 at 2411.491 kb on - strand, within Pf6N2E2_2022 at 2411.491 kb on - strand, within Pf6N2E2_2022 at 2411.491 kb on - strand, within Pf6N2E2_2022 at 2411.540 kb on + strand, within Pf6N2E2_2022 at 2411.540 kb on + strand, within Pf6N2E2_2022 at 2411.541 kb on - strand, within Pf6N2E2_2022 at 2411.541 kb on - strand, within Pf6N2E2_2022 at 2411.541 kb on - strand, within Pf6N2E2_2022 at 2411.667 kb on + strand, within Pf6N2E2_2022 at 2411.667 kb on + strand, within Pf6N2E2_2022 at 2411.667 kb on + strand, within Pf6N2E2_2022 at 2411.667 kb on + strand, within Pf6N2E2_2022 at 2411.667 kb on + strand, within Pf6N2E2_2022 at 2411.668 kb on - strand, within Pf6N2E2_2022 at 2411.668 kb on - strand, within Pf6N2E2_2022 at 2411.951 kb on + strand at 2411.951 kb on + strand at 2411.952 kb on - strand at 2411.986 kb on + strand at 2411.986 kb on + strand at 2411.987 kb on - strand at 2411.987 kb on - strand at 2412.032 kb on - strand at 2412.083 kb on - strand at 2412.106 kb on - strand at 2412.122 kb on + strand at 2412.122 kb on + strand at 2412.123 kb on - strand at 2412.238 kb on + strand, within Pf6N2E2_2023 at 2412.238 kb on + strand, within Pf6N2E2_2023 at 2412.238 kb on + strand, within Pf6N2E2_2023 at 2412.239 kb on - strand, within Pf6N2E2_2023 at 2412.366 kb on + strand, within Pf6N2E2_2023 at 2412.385 kb on + strand, within Pf6N2E2_2023 at 2412.385 kb on + strand, within Pf6N2E2_2023 at 2412.385 kb on + strand, within Pf6N2E2_2023 at 2412.385 kb on + strand, within Pf6N2E2_2023 at 2412.385 kb on + strand, within Pf6N2E2_2023 at 2412.386 kb on - strand, within Pf6N2E2_2023 at 2412.386 kb on - strand, within Pf6N2E2_2023 at 2412.386 kb on - strand, within Pf6N2E2_2023 at 2412.386 kb on - strand, within Pf6N2E2_2023 at 2412.386 kb on - strand, within Pf6N2E2_2023 at 2412.386 kb on - strand, within Pf6N2E2_2023 at 2412.520 kb on + strand, within Pf6N2E2_2023 at 2412.520 kb on + strand, within Pf6N2E2_2023 at 2412.520 kb on + strand, within Pf6N2E2_2023 at 2412.521 kb on - strand, within Pf6N2E2_2023 at 2412.812 kb on + strand, within Pf6N2E2_2024 at 2412.813 kb on - strand, within Pf6N2E2_2024 at 2412.813 kb on - strand, within Pf6N2E2_2024 at 2412.813 kb on - strand, within Pf6N2E2_2024 at 2412.842 kb on + strand, within Pf6N2E2_2024 at 2412.842 kb on + strand, within Pf6N2E2_2024 at 2412.875 kb on + strand, within Pf6N2E2_2024 at 2412.876 kb on - strand, within Pf6N2E2_2024 at 2412.876 kb on - strand, within Pf6N2E2_2024 at 2412.876 kb on - strand, within Pf6N2E2_2024 at 2412.882 kb on + strand, within Pf6N2E2_2024
Per-strain Table
Position Strand Gene LocusTag Fraction Glycerol (C) remove 2,410,486 + Pf6N2E2_2021 0.52 -0.3 2,410,486 + Pf6N2E2_2021 0.52 -0.1 2,410,487 - Pf6N2E2_2021 0.52 +0.2 2,410,487 - Pf6N2E2_2021 0.52 +0.8 2,410,487 - Pf6N2E2_2021 0.52 -0.5 2,410,489 + Pf6N2E2_2021 0.52 +0.4 2,410,835 - Pf6N2E2_2021 0.72 +1.0 2,410,889 - Pf6N2E2_2021 0.75 -0.5 2,410,918 + Pf6N2E2_2021 0.77 -0.1 2,410,918 + Pf6N2E2_2021 0.77 +0.5 2,410,919 - Pf6N2E2_2021 0.77 -0.5 2,410,921 + Pf6N2E2_2021 0.77 -1.3 2,410,921 + Pf6N2E2_2021 0.77 -0.1 2,410,921 + Pf6N2E2_2021 0.77 +0.2 2,410,922 - Pf6N2E2_2021 0.77 -0.4 2,410,922 - Pf6N2E2_2021 0.77 -0.5 2,411,011 + Pf6N2E2_2021 0.82 +0.7 2,411,011 + Pf6N2E2_2021 0.82 +0.5 2,411,083 + Pf6N2E2_2021 0.86 +0.3 2,411,090 - Pf6N2E2_2021 0.86 -0.2 2,411,119 + Pf6N2E2_2021 0.88 -1.5 2,411,119 + Pf6N2E2_2021 0.88 -0.6 2,411,257 + -0.3 2,411,258 - -2.5 2,411,299 + +0.1 2,411,300 - +1.2 2,411,480 - Pf6N2E2_2022 0.14 -1.5 2,411,490 + Pf6N2E2_2022 0.16 -1.5 2,411,490 + Pf6N2E2_2022 0.16 -1.0 2,411,491 - Pf6N2E2_2022 0.16 -1.0 2,411,491 - Pf6N2E2_2022 0.16 -1.8 2,411,491 - Pf6N2E2_2022 0.16 -1.1 2,411,491 - Pf6N2E2_2022 0.16 -3.0 2,411,540 + Pf6N2E2_2022 0.25 -1.1 2,411,540 + Pf6N2E2_2022 0.25 -0.1 2,411,541 - Pf6N2E2_2022 0.25 -0.1 2,411,541 - Pf6N2E2_2022 0.25 -0.8 2,411,541 - Pf6N2E2_2022 0.25 +0.8 2,411,667 + Pf6N2E2_2022 0.46 +0.2 2,411,667 + Pf6N2E2_2022 0.46 -0.2 2,411,667 + Pf6N2E2_2022 0.46 -0.1 2,411,667 + Pf6N2E2_2022 0.46 -0.4 2,411,667 + Pf6N2E2_2022 0.46 -1.8 2,411,668 - Pf6N2E2_2022 0.46 -2.7 2,411,668 - Pf6N2E2_2022 0.46 -3.8 2,411,951 + -1.9 2,411,951 + -2.0 2,411,952 - -1.1 2,411,986 + -1.5 2,411,986 + -1.9 2,411,987 - -2.4 2,411,987 - +0.1 2,412,032 - -1.3 2,412,083 - -0.5 2,412,106 - -0.6 2,412,122 + -1.0 2,412,122 + -1.9 2,412,123 - -0.6 2,412,238 + Pf6N2E2_2023 0.17 -1.3 2,412,238 + Pf6N2E2_2023 0.17 -0.6 2,412,238 + Pf6N2E2_2023 0.17 +0.8 2,412,239 - Pf6N2E2_2023 0.17 -0.8 2,412,366 + Pf6N2E2_2023 0.46 -0.3 2,412,385 + Pf6N2E2_2023 0.51 -0.6 2,412,385 + Pf6N2E2_2023 0.51 -0.3 2,412,385 + Pf6N2E2_2023 0.51 +0.0 2,412,385 + Pf6N2E2_2023 0.51 +0.7 2,412,385 + Pf6N2E2_2023 0.51 -1.1 2,412,386 - Pf6N2E2_2023 0.51 +1.2 2,412,386 - Pf6N2E2_2023 0.51 -0.4 2,412,386 - Pf6N2E2_2023 0.51 +0.7 2,412,386 - Pf6N2E2_2023 0.51 +1.2 2,412,386 - Pf6N2E2_2023 0.51 +0.1 2,412,386 - Pf6N2E2_2023 0.51 -0.5 2,412,520 + Pf6N2E2_2023 0.82 +0.9 2,412,520 + Pf6N2E2_2023 0.82 -3.2 2,412,520 + Pf6N2E2_2023 0.82 -1.6 2,412,521 - Pf6N2E2_2023 0.82 +0.1 2,412,812 + Pf6N2E2_2024 0.27 +0.4 2,412,813 - Pf6N2E2_2024 0.27 +1.0 2,412,813 - Pf6N2E2_2024 0.27 +0.2 2,412,813 - Pf6N2E2_2024 0.27 +0.6 2,412,842 + Pf6N2E2_2024 0.31 +0.9 2,412,842 + Pf6N2E2_2024 0.31 -0.5 2,412,875 + Pf6N2E2_2024 0.35 +0.2 2,412,876 - Pf6N2E2_2024 0.35 +0.1 2,412,876 - Pf6N2E2_2024 0.35 -0.2 2,412,876 - Pf6N2E2_2024 0.35 +0.7 2,412,882 + Pf6N2E2_2024 0.36 +0.1
Or see this region's nucleotide sequence