Experiment: R2A with Nalidixic 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS07955 and CA265_RS07960 are separated by 4 nucleotides CA265_RS07960 and CA265_RS07965 are separated by 13 nucleotides CA265_RS07965 and CA265_RS07970 are separated by 117 nucleotides
CA265_RS07955: CA265_RS07955 - asparagine synthase (glutamine-hydrolyzing), at 1,984,137 to 1,985,978
_RS07955
CA265_RS07960: CA265_RS07960 - hypothetical protein, at 1,985,983 to 1,986,873
_RS07960
CA265_RS07965: CA265_RS07965 - hypothetical protein, at 1,986,887 to 1,987,969
_RS07965
CA265_RS07970: CA265_RS07970 - NAD-dependent epimerase, at 1,988,087 to 1,989,178
_RS07970
Position (kb)
1986
1987
1988 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1985.957 kb on + strand at 1985.958 kb on - strand at 1985.968 kb on + strand at 1986.127 kb on + strand, within CA265_RS07960 at 1986.155 kb on + strand, within CA265_RS07960 at 1986.216 kb on + strand, within CA265_RS07960 at 1986.217 kb on - strand, within CA265_RS07960 at 1986.288 kb on - strand, within CA265_RS07960 at 1986.323 kb on + strand, within CA265_RS07960 at 1986.324 kb on - strand, within CA265_RS07960 at 1986.414 kb on - strand, within CA265_RS07960 at 1986.455 kb on - strand, within CA265_RS07960 at 1986.481 kb on - strand, within CA265_RS07960 at 1986.488 kb on + strand, within CA265_RS07960 at 1986.518 kb on - strand, within CA265_RS07960 at 1986.544 kb on + strand, within CA265_RS07960 at 1986.544 kb on + strand, within CA265_RS07960 at 1986.545 kb on - strand, within CA265_RS07960 at 1986.546 kb on + strand at 1986.557 kb on - strand, within CA265_RS07960 at 1986.661 kb on + strand, within CA265_RS07960 at 1986.726 kb on + strand, within CA265_RS07960 at 1986.737 kb on + strand, within CA265_RS07960 at 1986.754 kb on + strand, within CA265_RS07960 at 1986.795 kb on + strand at 1986.800 kb on + strand at 1986.803 kb on + strand at 1986.804 kb on - strand at 1986.804 kb on - strand at 1986.872 kb on - strand at 1986.991 kb on - strand at 1987.052 kb on + strand, within CA265_RS07965 at 1987.086 kb on + strand, within CA265_RS07965 at 1987.115 kb on - strand, within CA265_RS07965 at 1987.120 kb on + strand, within CA265_RS07965 at 1987.181 kb on - strand, within CA265_RS07965 at 1987.182 kb on + strand, within CA265_RS07965 at 1987.183 kb on - strand, within CA265_RS07965 at 1987.198 kb on + strand, within CA265_RS07965 at 1987.220 kb on - strand, within CA265_RS07965 at 1987.373 kb on - strand, within CA265_RS07965 at 1987.375 kb on - strand, within CA265_RS07965 at 1987.406 kb on - strand, within CA265_RS07965 at 1987.438 kb on + strand, within CA265_RS07965 at 1987.438 kb on + strand, within CA265_RS07965 at 1987.522 kb on + strand, within CA265_RS07965 at 1987.620 kb on + strand, within CA265_RS07965 at 1987.651 kb on - strand, within CA265_RS07965 at 1987.654 kb on + strand, within CA265_RS07965 at 1987.725 kb on + strand, within CA265_RS07965 at 1987.726 kb on - strand, within CA265_RS07965 at 1987.726 kb on - strand, within CA265_RS07965 at 1987.726 kb on - strand, within CA265_RS07965 at 1987.758 kb on + strand, within CA265_RS07965 at 1987.761 kb on + strand, within CA265_RS07965 at 1987.762 kb on - strand, within CA265_RS07965 at 1987.784 kb on - strand, within CA265_RS07965 at 1987.827 kb on + strand, within CA265_RS07965 at 1987.827 kb on + strand, within CA265_RS07965 at 1987.828 kb on - strand, within CA265_RS07965 at 1987.828 kb on - strand, within CA265_RS07965 at 1987.902 kb on - strand at 1987.905 kb on + strand at 1987.905 kb on + strand at 1987.910 kb on - strand at 1987.953 kb on + strand at 1988.003 kb on + strand at 1988.003 kb on + strand at 1988.180 kb on - strand at 1988.202 kb on + strand, within CA265_RS07970 at 1988.209 kb on - strand, within CA265_RS07970 at 1988.240 kb on - strand, within CA265_RS07970 at 1988.274 kb on + strand, within CA265_RS07970 at 1988.282 kb on - strand, within CA265_RS07970 at 1988.286 kb on - strand, within CA265_RS07970 at 1988.286 kb on - strand, within CA265_RS07970 at 1988.294 kb on + strand, within CA265_RS07970 at 1988.295 kb on - strand, within CA265_RS07970 at 1988.409 kb on + strand, within CA265_RS07970 at 1988.410 kb on - strand, within CA265_RS07970 at 1988.413 kb on + strand, within CA265_RS07970 at 1988.427 kb on - strand, within CA265_RS07970 at 1988.431 kb on - strand, within CA265_RS07970 at 1988.454 kb on + strand, within CA265_RS07970 at 1988.501 kb on + strand, within CA265_RS07970 at 1988.502 kb on - strand, within CA265_RS07970 at 1988.585 kb on + strand, within CA265_RS07970 at 1988.627 kb on + strand, within CA265_RS07970 at 1988.627 kb on + strand, within CA265_RS07970 at 1988.628 kb on - strand, within CA265_RS07970 at 1988.654 kb on - strand, within CA265_RS07970 at 1988.654 kb on - strand, within CA265_RS07970 at 1988.662 kb on - strand, within CA265_RS07970 at 1988.678 kb on + strand, within CA265_RS07970 at 1988.678 kb on + strand, within CA265_RS07970 at 1988.679 kb on - strand, within CA265_RS07970 at 1988.720 kb on - strand, within CA265_RS07970 at 1988.761 kb on + strand, within CA265_RS07970 at 1988.785 kb on + strand, within CA265_RS07970 at 1988.790 kb on - strand, within CA265_RS07970 at 1988.793 kb on + strand, within CA265_RS07970 at 1988.794 kb on - strand, within CA265_RS07970 at 1988.800 kb on + strand, within CA265_RS07970 at 1988.803 kb on + strand, within CA265_RS07970 at 1988.823 kb on + strand, within CA265_RS07970 at 1988.882 kb on - strand, within CA265_RS07970
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nalidixic 0.008 mg/ml remove 1,985,957 + -0.4 1,985,958 - +0.3 1,985,968 + -0.0 1,986,127 + CA265_RS07960 0.16 -0.6 1,986,155 + CA265_RS07960 0.19 -0.3 1,986,216 + CA265_RS07960 0.26 -0.8 1,986,217 - CA265_RS07960 0.26 +0.1 1,986,288 - CA265_RS07960 0.34 -0.3 1,986,323 + CA265_RS07960 0.38 -0.3 1,986,324 - CA265_RS07960 0.38 +0.3 1,986,414 - CA265_RS07960 0.48 -0.2 1,986,455 - CA265_RS07960 0.53 -2.0 1,986,481 - CA265_RS07960 0.56 +0.4 1,986,488 + CA265_RS07960 0.57 +0.1 1,986,518 - CA265_RS07960 0.60 +0.8 1,986,544 + CA265_RS07960 0.63 -0.1 1,986,544 + CA265_RS07960 0.63 -0.2 1,986,545 - CA265_RS07960 0.63 -1.0 1,986,546 + +0.9 1,986,557 - CA265_RS07960 0.64 -0.1 1,986,661 + CA265_RS07960 0.76 -0.3 1,986,726 + CA265_RS07960 0.83 +0.0 1,986,737 + CA265_RS07960 0.85 -0.1 1,986,754 + CA265_RS07960 0.87 +0.1 1,986,795 + +0.5 1,986,800 + -0.1 1,986,803 + -2.5 1,986,804 - -0.6 1,986,804 - +1.4 1,986,872 - -0.0 1,986,991 - +0.0 1,987,052 + CA265_RS07965 0.15 -0.5 1,987,086 + CA265_RS07965 0.18 +0.6 1,987,115 - CA265_RS07965 0.21 -0.1 1,987,120 + CA265_RS07965 0.22 -0.3 1,987,181 - CA265_RS07965 0.27 -0.1 1,987,182 + CA265_RS07965 0.27 -0.8 1,987,183 - CA265_RS07965 0.27 +0.2 1,987,198 + CA265_RS07965 0.29 +0.7 1,987,220 - CA265_RS07965 0.31 -0.1 1,987,373 - CA265_RS07965 0.45 -1.1 1,987,375 - CA265_RS07965 0.45 +0.8 1,987,406 - CA265_RS07965 0.48 +0.3 1,987,438 + CA265_RS07965 0.51 -0.1 1,987,438 + CA265_RS07965 0.51 +1.2 1,987,522 + CA265_RS07965 0.59 +0.2 1,987,620 + CA265_RS07965 0.68 +0.1 1,987,651 - CA265_RS07965 0.71 +0.1 1,987,654 + CA265_RS07965 0.71 +0.4 1,987,725 + CA265_RS07965 0.77 +0.3 1,987,726 - CA265_RS07965 0.77 +0.7 1,987,726 - CA265_RS07965 0.77 -0.0 1,987,726 - CA265_RS07965 0.77 +0.2 1,987,758 + CA265_RS07965 0.80 -0.5 1,987,761 + CA265_RS07965 0.81 -0.1 1,987,762 - CA265_RS07965 0.81 +0.2 1,987,784 - CA265_RS07965 0.83 +0.0 1,987,827 + CA265_RS07965 0.87 -0.2 1,987,827 + CA265_RS07965 0.87 +0.3 1,987,828 - CA265_RS07965 0.87 +0.0 1,987,828 - CA265_RS07965 0.87 -0.1 1,987,902 - +0.0 1,987,905 + +0.1 1,987,905 + +1.2 1,987,910 - -0.4 1,987,953 + +0.1 1,988,003 + -0.5 1,988,003 + +0.0 1,988,180 - +0.1 1,988,202 + CA265_RS07970 0.11 +0.5 1,988,209 - CA265_RS07970 0.11 +1.9 1,988,240 - CA265_RS07970 0.14 +0.0 1,988,274 + CA265_RS07970 0.17 +0.7 1,988,282 - CA265_RS07970 0.18 +0.4 1,988,286 - CA265_RS07970 0.18 +0.2 1,988,286 - CA265_RS07970 0.18 -0.1 1,988,294 + CA265_RS07970 0.19 +0.0 1,988,295 - CA265_RS07970 0.19 +0.6 1,988,409 + CA265_RS07970 0.29 +0.5 1,988,410 - CA265_RS07970 0.30 -0.1 1,988,413 + CA265_RS07970 0.30 +1.0 1,988,427 - CA265_RS07970 0.31 -0.1 1,988,431 - CA265_RS07970 0.32 -0.2 1,988,454 + CA265_RS07970 0.34 -0.1 1,988,501 + CA265_RS07970 0.38 -0.1 1,988,502 - CA265_RS07970 0.38 +0.7 1,988,585 + CA265_RS07970 0.46 -0.3 1,988,627 + CA265_RS07970 0.49 +0.1 1,988,627 + CA265_RS07970 0.49 -0.9 1,988,628 - CA265_RS07970 0.50 -0.0 1,988,654 - CA265_RS07970 0.52 +0.3 1,988,654 - CA265_RS07970 0.52 +0.0 1,988,662 - CA265_RS07970 0.53 +0.4 1,988,678 + CA265_RS07970 0.54 -0.3 1,988,678 + CA265_RS07970 0.54 +0.1 1,988,679 - CA265_RS07970 0.54 -0.2 1,988,720 - CA265_RS07970 0.58 -0.1 1,988,761 + CA265_RS07970 0.62 +0.3 1,988,785 + CA265_RS07970 0.64 -0.1 1,988,790 - CA265_RS07970 0.64 -0.2 1,988,793 + CA265_RS07970 0.65 +0.2 1,988,794 - CA265_RS07970 0.65 -0.3 1,988,800 + CA265_RS07970 0.65 -0.3 1,988,803 + CA265_RS07970 0.66 +0.1 1,988,823 + CA265_RS07970 0.67 -0.5 1,988,882 - CA265_RS07970 0.73 +0.7
Or see this region's nucleotide sequence