Experiment: R2A with Nalidixic 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS07955 and CA265_RS07960 are separated by 4 nucleotides CA265_RS07960 and CA265_RS07965 are separated by 13 nucleotides
CA265_RS07955: CA265_RS07955 - asparagine synthase (glutamine-hydrolyzing), at 1,984,137 to 1,985,978
_RS07955
CA265_RS07960: CA265_RS07960 - hypothetical protein, at 1,985,983 to 1,986,873
_RS07960
CA265_RS07965: CA265_RS07965 - hypothetical protein, at 1,986,887 to 1,987,969
_RS07965
Position (kb)
1985
1986
1987 Strain fitness (log2 ratio)
-2
-1
0
1 at 1985.019 kb on + strand, within CA265_RS07955 at 1985.058 kb on + strand, within CA265_RS07955 at 1985.058 kb on + strand, within CA265_RS07955 at 1985.060 kb on + strand, within CA265_RS07955 at 1985.077 kb on - strand, within CA265_RS07955 at 1985.096 kb on - strand, within CA265_RS07955 at 1985.157 kb on - strand, within CA265_RS07955 at 1985.263 kb on + strand, within CA265_RS07955 at 1985.264 kb on - strand, within CA265_RS07955 at 1985.387 kb on - strand, within CA265_RS07955 at 1985.406 kb on - strand, within CA265_RS07955 at 1985.448 kb on + strand, within CA265_RS07955 at 1985.448 kb on + strand, within CA265_RS07955 at 1985.448 kb on + strand, within CA265_RS07955 at 1985.465 kb on + strand, within CA265_RS07955 at 1985.465 kb on + strand, within CA265_RS07955 at 1985.492 kb on - strand, within CA265_RS07955 at 1985.546 kb on + strand, within CA265_RS07955 at 1985.547 kb on - strand, within CA265_RS07955 at 1985.547 kb on - strand, within CA265_RS07955 at 1985.547 kb on - strand, within CA265_RS07955 at 1985.652 kb on + strand, within CA265_RS07955 at 1985.655 kb on - strand, within CA265_RS07955 at 1985.689 kb on + strand, within CA265_RS07955 at 1985.715 kb on - strand, within CA265_RS07955 at 1985.715 kb on - strand, within CA265_RS07955 at 1985.858 kb on + strand at 1985.870 kb on + strand at 1985.871 kb on - strand at 1985.871 kb on - strand at 1985.957 kb on + strand at 1985.958 kb on - strand at 1985.968 kb on + strand at 1986.127 kb on + strand, within CA265_RS07960 at 1986.155 kb on + strand, within CA265_RS07960 at 1986.216 kb on + strand, within CA265_RS07960 at 1986.217 kb on - strand, within CA265_RS07960 at 1986.288 kb on - strand, within CA265_RS07960 at 1986.323 kb on + strand, within CA265_RS07960 at 1986.324 kb on - strand, within CA265_RS07960 at 1986.414 kb on - strand, within CA265_RS07960 at 1986.455 kb on - strand, within CA265_RS07960 at 1986.481 kb on - strand, within CA265_RS07960 at 1986.488 kb on + strand, within CA265_RS07960 at 1986.518 kb on - strand, within CA265_RS07960 at 1986.544 kb on + strand, within CA265_RS07960 at 1986.544 kb on + strand, within CA265_RS07960 at 1986.545 kb on - strand, within CA265_RS07960 at 1986.546 kb on + strand at 1986.557 kb on - strand, within CA265_RS07960 at 1986.661 kb on + strand, within CA265_RS07960 at 1986.726 kb on + strand, within CA265_RS07960 at 1986.737 kb on + strand, within CA265_RS07960 at 1986.754 kb on + strand, within CA265_RS07960 at 1986.795 kb on + strand at 1986.800 kb on + strand at 1986.803 kb on + strand at 1986.804 kb on - strand at 1986.804 kb on - strand at 1986.872 kb on - strand at 1986.991 kb on - strand at 1987.052 kb on + strand, within CA265_RS07965 at 1987.086 kb on + strand, within CA265_RS07965 at 1987.115 kb on - strand, within CA265_RS07965 at 1987.120 kb on + strand, within CA265_RS07965 at 1987.181 kb on - strand, within CA265_RS07965 at 1987.182 kb on + strand, within CA265_RS07965 at 1987.183 kb on - strand, within CA265_RS07965 at 1987.198 kb on + strand, within CA265_RS07965 at 1987.220 kb on - strand, within CA265_RS07965 at 1987.373 kb on - strand, within CA265_RS07965 at 1987.375 kb on - strand, within CA265_RS07965 at 1987.406 kb on - strand, within CA265_RS07965 at 1987.438 kb on + strand, within CA265_RS07965 at 1987.438 kb on + strand, within CA265_RS07965 at 1987.522 kb on + strand, within CA265_RS07965 at 1987.620 kb on + strand, within CA265_RS07965 at 1987.651 kb on - strand, within CA265_RS07965 at 1987.654 kb on + strand, within CA265_RS07965 at 1987.725 kb on + strand, within CA265_RS07965 at 1987.726 kb on - strand, within CA265_RS07965 at 1987.726 kb on - strand, within CA265_RS07965 at 1987.726 kb on - strand, within CA265_RS07965 at 1987.758 kb on + strand, within CA265_RS07965 at 1987.761 kb on + strand, within CA265_RS07965 at 1987.762 kb on - strand, within CA265_RS07965 at 1987.784 kb on - strand, within CA265_RS07965 at 1987.827 kb on + strand, within CA265_RS07965 at 1987.827 kb on + strand, within CA265_RS07965 at 1987.828 kb on - strand, within CA265_RS07965 at 1987.828 kb on - strand, within CA265_RS07965
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nalidixic 0.008 mg/ml remove 1,985,019 + CA265_RS07955 0.48 -0.1 1,985,058 + CA265_RS07955 0.50 +0.3 1,985,058 + CA265_RS07955 0.50 +0.6 1,985,060 + CA265_RS07955 0.50 +0.1 1,985,077 - CA265_RS07955 0.51 +0.3 1,985,096 - CA265_RS07955 0.52 +0.4 1,985,157 - CA265_RS07955 0.55 +1.2 1,985,263 + CA265_RS07955 0.61 +0.1 1,985,264 - CA265_RS07955 0.61 +0.5 1,985,387 - CA265_RS07955 0.68 +0.8 1,985,406 - CA265_RS07955 0.69 -0.5 1,985,448 + CA265_RS07955 0.71 -0.4 1,985,448 + CA265_RS07955 0.71 -0.5 1,985,448 + CA265_RS07955 0.71 -1.0 1,985,465 + CA265_RS07955 0.72 -0.2 1,985,465 + CA265_RS07955 0.72 +1.3 1,985,492 - CA265_RS07955 0.74 -0.1 1,985,546 + CA265_RS07955 0.76 -0.9 1,985,547 - CA265_RS07955 0.77 +0.1 1,985,547 - CA265_RS07955 0.77 +0.3 1,985,547 - CA265_RS07955 0.77 +1.1 1,985,652 + CA265_RS07955 0.82 -0.8 1,985,655 - CA265_RS07955 0.82 +0.2 1,985,689 + CA265_RS07955 0.84 -0.3 1,985,715 - CA265_RS07955 0.86 +0.0 1,985,715 - CA265_RS07955 0.86 +0.1 1,985,858 + -0.7 1,985,870 + +0.2 1,985,871 - +0.3 1,985,871 - +0.3 1,985,957 + -0.4 1,985,958 - +0.3 1,985,968 + -0.0 1,986,127 + CA265_RS07960 0.16 -0.6 1,986,155 + CA265_RS07960 0.19 -0.3 1,986,216 + CA265_RS07960 0.26 -0.8 1,986,217 - CA265_RS07960 0.26 +0.1 1,986,288 - CA265_RS07960 0.34 -0.3 1,986,323 + CA265_RS07960 0.38 -0.3 1,986,324 - CA265_RS07960 0.38 +0.3 1,986,414 - CA265_RS07960 0.48 -0.2 1,986,455 - CA265_RS07960 0.53 -2.0 1,986,481 - CA265_RS07960 0.56 +0.4 1,986,488 + CA265_RS07960 0.57 +0.1 1,986,518 - CA265_RS07960 0.60 +0.8 1,986,544 + CA265_RS07960 0.63 -0.1 1,986,544 + CA265_RS07960 0.63 -0.2 1,986,545 - CA265_RS07960 0.63 -1.0 1,986,546 + +0.9 1,986,557 - CA265_RS07960 0.64 -0.1 1,986,661 + CA265_RS07960 0.76 -0.3 1,986,726 + CA265_RS07960 0.83 +0.0 1,986,737 + CA265_RS07960 0.85 -0.1 1,986,754 + CA265_RS07960 0.87 +0.1 1,986,795 + +0.5 1,986,800 + -0.1 1,986,803 + -2.5 1,986,804 - -0.6 1,986,804 - +1.4 1,986,872 - -0.0 1,986,991 - +0.0 1,987,052 + CA265_RS07965 0.15 -0.5 1,987,086 + CA265_RS07965 0.18 +0.6 1,987,115 - CA265_RS07965 0.21 -0.1 1,987,120 + CA265_RS07965 0.22 -0.3 1,987,181 - CA265_RS07965 0.27 -0.1 1,987,182 + CA265_RS07965 0.27 -0.8 1,987,183 - CA265_RS07965 0.27 +0.2 1,987,198 + CA265_RS07965 0.29 +0.7 1,987,220 - CA265_RS07965 0.31 -0.1 1,987,373 - CA265_RS07965 0.45 -1.1 1,987,375 - CA265_RS07965 0.45 +0.8 1,987,406 - CA265_RS07965 0.48 +0.3 1,987,438 + CA265_RS07965 0.51 -0.1 1,987,438 + CA265_RS07965 0.51 +1.2 1,987,522 + CA265_RS07965 0.59 +0.2 1,987,620 + CA265_RS07965 0.68 +0.1 1,987,651 - CA265_RS07965 0.71 +0.1 1,987,654 + CA265_RS07965 0.71 +0.4 1,987,725 + CA265_RS07965 0.77 +0.3 1,987,726 - CA265_RS07965 0.77 +0.7 1,987,726 - CA265_RS07965 0.77 -0.0 1,987,726 - CA265_RS07965 0.77 +0.2 1,987,758 + CA265_RS07965 0.80 -0.5 1,987,761 + CA265_RS07965 0.81 -0.1 1,987,762 - CA265_RS07965 0.81 +0.2 1,987,784 - CA265_RS07965 0.83 +0.0 1,987,827 + CA265_RS07965 0.87 -0.2 1,987,827 + CA265_RS07965 0.87 +0.3 1,987,828 - CA265_RS07965 0.87 +0.0 1,987,828 - CA265_RS07965 0.87 -0.1
Or see this region's nucleotide sequence