Experiment: R2A with Nalidixic 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS05465 and CA265_RS05470 are separated by 161 nucleotides CA265_RS05470 and CA265_RS05475 are separated by 27 nucleotides
CA265_RS05465: CA265_RS05465 - sulfatase, at 1,319,293 to 1,320,438
_RS05465
CA265_RS05470: CA265_RS05470 - SusC/RagA family protein, at 1,320,600 to 1,323,713
_RS05470
CA265_RS05475: CA265_RS05475 - hypothetical protein, at 1,323,741 to 1,325,261
_RS05475
Position (kb)
1320
1321
1322
1323
1324 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1319.603 kb on + strand, within CA265_RS05465 at 1319.621 kb on - strand, within CA265_RS05465 at 1319.639 kb on + strand, within CA265_RS05465 at 1319.681 kb on - strand, within CA265_RS05465 at 1319.681 kb on - strand, within CA265_RS05465 at 1319.682 kb on + strand, within CA265_RS05465 at 1319.683 kb on - strand, within CA265_RS05465 at 1319.742 kb on + strand, within CA265_RS05465 at 1319.743 kb on - strand, within CA265_RS05465 at 1319.748 kb on + strand, within CA265_RS05465 at 1319.905 kb on - strand, within CA265_RS05465 at 1319.905 kb on - strand, within CA265_RS05465 at 1319.908 kb on + strand, within CA265_RS05465 at 1319.942 kb on + strand, within CA265_RS05465 at 1319.952 kb on + strand, within CA265_RS05465 at 1320.029 kb on - strand, within CA265_RS05465 at 1320.125 kb on - strand, within CA265_RS05465 at 1320.160 kb on - strand, within CA265_RS05465 at 1320.163 kb on + strand, within CA265_RS05465 at 1320.173 kb on - strand, within CA265_RS05465 at 1320.192 kb on - strand, within CA265_RS05465 at 1320.192 kb on - strand, within CA265_RS05465 at 1320.211 kb on + strand, within CA265_RS05465 at 1320.346 kb on + strand at 1320.347 kb on - strand at 1320.436 kb on + strand at 1320.530 kb on - strand at 1320.578 kb on + strand at 1320.619 kb on + strand at 1320.619 kb on + strand at 1320.728 kb on - strand at 1320.742 kb on + strand at 1320.877 kb on + strand at 1320.884 kb on + strand at 1320.888 kb on - strand at 1320.888 kb on - strand at 1320.935 kb on + strand, within CA265_RS05470 at 1321.019 kb on + strand, within CA265_RS05470 at 1321.019 kb on + strand, within CA265_RS05470 at 1321.020 kb on - strand, within CA265_RS05470 at 1321.020 kb on - strand, within CA265_RS05470 at 1321.020 kb on - strand, within CA265_RS05470 at 1321.033 kb on + strand, within CA265_RS05470 at 1321.033 kb on + strand, within CA265_RS05470 at 1321.040 kb on - strand, within CA265_RS05470 at 1321.229 kb on + strand, within CA265_RS05470 at 1321.230 kb on - strand, within CA265_RS05470 at 1321.258 kb on + strand, within CA265_RS05470 at 1321.370 kb on - strand, within CA265_RS05470 at 1321.379 kb on + strand, within CA265_RS05470 at 1321.379 kb on + strand, within CA265_RS05470 at 1321.380 kb on - strand, within CA265_RS05470 at 1321.479 kb on + strand, within CA265_RS05470 at 1321.479 kb on + strand, within CA265_RS05470 at 1321.480 kb on - strand, within CA265_RS05470 at 1321.480 kb on - strand, within CA265_RS05470 at 1321.491 kb on + strand, within CA265_RS05470 at 1321.519 kb on + strand, within CA265_RS05470 at 1321.520 kb on - strand, within CA265_RS05470 at 1321.525 kb on + strand, within CA265_RS05470 at 1321.525 kb on + strand, within CA265_RS05470 at 1321.593 kb on + strand, within CA265_RS05470 at 1321.718 kb on - strand, within CA265_RS05470 at 1321.718 kb on - strand, within CA265_RS05470 at 1321.718 kb on - strand, within CA265_RS05470 at 1321.750 kb on + strand, within CA265_RS05470 at 1321.789 kb on + strand, within CA265_RS05470 at 1321.793 kb on + strand, within CA265_RS05470 at 1321.834 kb on - strand, within CA265_RS05470 at 1321.847 kb on + strand, within CA265_RS05470 at 1321.847 kb on + strand, within CA265_RS05470 at 1321.847 kb on + strand, within CA265_RS05470 at 1321.848 kb on - strand, within CA265_RS05470 at 1321.850 kb on - strand, within CA265_RS05470 at 1321.928 kb on + strand, within CA265_RS05470 at 1321.953 kb on - strand, within CA265_RS05470 at 1321.981 kb on + strand, within CA265_RS05470 at 1321.982 kb on - strand, within CA265_RS05470 at 1321.985 kb on + strand, within CA265_RS05470 at 1321.985 kb on + strand, within CA265_RS05470 at 1321.986 kb on - strand, within CA265_RS05470 at 1321.986 kb on - strand, within CA265_RS05470 at 1321.990 kb on + strand, within CA265_RS05470 at 1322.049 kb on + strand, within CA265_RS05470 at 1322.049 kb on + strand, within CA265_RS05470 at 1322.050 kb on - strand, within CA265_RS05470 at 1322.052 kb on - strand, within CA265_RS05470 at 1322.052 kb on - strand, within CA265_RS05470 at 1322.052 kb on - strand, within CA265_RS05470 at 1322.122 kb on - strand, within CA265_RS05470 at 1322.122 kb on - strand, within CA265_RS05470 at 1322.171 kb on - strand, within CA265_RS05470 at 1322.180 kb on - strand, within CA265_RS05470 at 1322.197 kb on + strand, within CA265_RS05470 at 1322.214 kb on - strand, within CA265_RS05470 at 1322.267 kb on + strand, within CA265_RS05470 at 1322.268 kb on - strand, within CA265_RS05470 at 1322.306 kb on + strand, within CA265_RS05470 at 1322.333 kb on + strand, within CA265_RS05470 at 1322.334 kb on - strand, within CA265_RS05470 at 1322.428 kb on - strand, within CA265_RS05470 at 1322.433 kb on + strand, within CA265_RS05470 at 1322.442 kb on - strand, within CA265_RS05470 at 1322.580 kb on - strand, within CA265_RS05470 at 1322.754 kb on + strand, within CA265_RS05470 at 1322.755 kb on - strand, within CA265_RS05470 at 1322.755 kb on - strand, within CA265_RS05470 at 1322.756 kb on + strand, within CA265_RS05470 at 1322.801 kb on - strand, within CA265_RS05470 at 1322.815 kb on - strand, within CA265_RS05470 at 1322.815 kb on - strand, within CA265_RS05470 at 1322.818 kb on - strand, within CA265_RS05470 at 1322.900 kb on - strand, within CA265_RS05470 at 1322.938 kb on + strand, within CA265_RS05470 at 1322.938 kb on + strand, within CA265_RS05470 at 1323.112 kb on - strand, within CA265_RS05470 at 1323.127 kb on + strand, within CA265_RS05470 at 1323.127 kb on + strand, within CA265_RS05470 at 1323.213 kb on - strand, within CA265_RS05470 at 1323.242 kb on - strand, within CA265_RS05470 at 1323.374 kb on - strand, within CA265_RS05470 at 1323.405 kb on - strand at 1323.412 kb on + strand at 1323.498 kb on - strand at 1323.506 kb on + strand at 1323.524 kb on - strand at 1323.550 kb on + strand at 1323.559 kb on - strand at 1323.595 kb on - strand at 1323.596 kb on + strand at 1323.616 kb on + strand at 1323.617 kb on - strand at 1323.667 kb on - strand at 1323.738 kb on - strand at 1323.753 kb on + strand at 1323.757 kb on + strand at 1323.757 kb on + strand at 1323.883 kb on + strand at 1323.885 kb on + strand at 1323.905 kb on + strand, within CA265_RS05475 at 1323.928 kb on - strand, within CA265_RS05475 at 1323.928 kb on - strand, within CA265_RS05475 at 1323.928 kb on - strand, within CA265_RS05475 at 1323.929 kb on + strand, within CA265_RS05475 at 1324.006 kb on + strand, within CA265_RS05475 at 1324.074 kb on + strand, within CA265_RS05475 at 1324.094 kb on + strand, within CA265_RS05475 at 1324.095 kb on - strand, within CA265_RS05475 at 1324.095 kb on - strand, within CA265_RS05475 at 1324.189 kb on - strand, within CA265_RS05475 at 1324.308 kb on - strand, within CA265_RS05475 at 1324.410 kb on + strand, within CA265_RS05475 at 1324.411 kb on - strand, within CA265_RS05475 at 1324.447 kb on - strand, within CA265_RS05475 at 1324.474 kb on - strand, within CA265_RS05475 at 1324.480 kb on - strand, within CA265_RS05475 at 1324.497 kb on + strand, within CA265_RS05475 at 1324.498 kb on - strand, within CA265_RS05475 at 1324.524 kb on - strand, within CA265_RS05475 at 1324.569 kb on - strand, within CA265_RS05475 at 1324.659 kb on + strand, within CA265_RS05475
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nalidixic 0.008 mg/ml remove 1,319,603 + CA265_RS05465 0.27 -0.4 1,319,621 - CA265_RS05465 0.29 +0.1 1,319,639 + CA265_RS05465 0.30 -1.0 1,319,681 - CA265_RS05465 0.34 -0.8 1,319,681 - CA265_RS05465 0.34 -0.3 1,319,682 + CA265_RS05465 0.34 -1.7 1,319,683 - CA265_RS05465 0.34 -0.6 1,319,742 + CA265_RS05465 0.39 -0.7 1,319,743 - CA265_RS05465 0.39 +0.3 1,319,748 + CA265_RS05465 0.40 +0.2 1,319,905 - CA265_RS05465 0.53 +0.2 1,319,905 - CA265_RS05465 0.53 +0.1 1,319,908 + CA265_RS05465 0.54 -0.5 1,319,942 + CA265_RS05465 0.57 +0.6 1,319,952 + CA265_RS05465 0.58 +1.0 1,320,029 - CA265_RS05465 0.64 -0.3 1,320,125 - CA265_RS05465 0.73 +0.0 1,320,160 - CA265_RS05465 0.76 -0.3 1,320,163 + CA265_RS05465 0.76 +0.2 1,320,173 - CA265_RS05465 0.77 -0.5 1,320,192 - CA265_RS05465 0.78 -0.4 1,320,192 - CA265_RS05465 0.78 +0.3 1,320,211 + CA265_RS05465 0.80 +0.3 1,320,346 + -0.2 1,320,347 - -0.1 1,320,436 + +0.1 1,320,530 - -0.4 1,320,578 + -0.1 1,320,619 + -0.5 1,320,619 + +0.0 1,320,728 - +0.1 1,320,742 + -0.1 1,320,877 + -0.6 1,320,884 + -0.2 1,320,888 - -1.0 1,320,888 - +0.1 1,320,935 + CA265_RS05470 0.11 -0.9 1,321,019 + CA265_RS05470 0.13 -0.1 1,321,019 + CA265_RS05470 0.13 -1.0 1,321,020 - CA265_RS05470 0.13 +0.1 1,321,020 - CA265_RS05470 0.13 -0.4 1,321,020 - CA265_RS05470 0.13 -1.2 1,321,033 + CA265_RS05470 0.14 -1.2 1,321,033 + CA265_RS05470 0.14 -0.1 1,321,040 - CA265_RS05470 0.14 -0.3 1,321,229 + CA265_RS05470 0.20 -0.6 1,321,230 - CA265_RS05470 0.20 +0.4 1,321,258 + CA265_RS05470 0.21 -1.7 1,321,370 - CA265_RS05470 0.25 -1.0 1,321,379 + CA265_RS05470 0.25 -0.4 1,321,379 + CA265_RS05470 0.25 +0.6 1,321,380 - CA265_RS05470 0.25 -0.4 1,321,479 + CA265_RS05470 0.28 -0.5 1,321,479 + CA265_RS05470 0.28 -0.4 1,321,480 - CA265_RS05470 0.28 +0.6 1,321,480 - CA265_RS05470 0.28 -0.7 1,321,491 + CA265_RS05470 0.29 -1.2 1,321,519 + CA265_RS05470 0.30 -0.9 1,321,520 - CA265_RS05470 0.30 +0.6 1,321,525 + CA265_RS05470 0.30 +0.9 1,321,525 + CA265_RS05470 0.30 -0.8 1,321,593 + CA265_RS05470 0.32 -1.0 1,321,718 - CA265_RS05470 0.36 -0.2 1,321,718 - CA265_RS05470 0.36 -1.3 1,321,718 - CA265_RS05470 0.36 -0.9 1,321,750 + CA265_RS05470 0.37 +0.2 1,321,789 + CA265_RS05470 0.38 +0.2 1,321,793 + CA265_RS05470 0.38 -0.2 1,321,834 - CA265_RS05470 0.40 +1.1 1,321,847 + CA265_RS05470 0.40 -0.4 1,321,847 + CA265_RS05470 0.40 -0.4 1,321,847 + CA265_RS05470 0.40 -0.2 1,321,848 - CA265_RS05470 0.40 -0.5 1,321,850 - CA265_RS05470 0.40 -0.1 1,321,928 + CA265_RS05470 0.43 +0.2 1,321,953 - CA265_RS05470 0.43 -0.1 1,321,981 + CA265_RS05470 0.44 -0.8 1,321,982 - CA265_RS05470 0.44 -3.4 1,321,985 + CA265_RS05470 0.44 -0.4 1,321,985 + CA265_RS05470 0.44 +0.1 1,321,986 - CA265_RS05470 0.45 +0.4 1,321,986 - CA265_RS05470 0.45 +0.2 1,321,990 + CA265_RS05470 0.45 +0.1 1,322,049 + CA265_RS05470 0.47 -1.0 1,322,049 + CA265_RS05470 0.47 +0.3 1,322,050 - CA265_RS05470 0.47 -0.2 1,322,052 - CA265_RS05470 0.47 +0.1 1,322,052 - CA265_RS05470 0.47 -0.4 1,322,052 - CA265_RS05470 0.47 -0.2 1,322,122 - CA265_RS05470 0.49 -0.5 1,322,122 - CA265_RS05470 0.49 -0.0 1,322,171 - CA265_RS05470 0.50 +0.1 1,322,180 - CA265_RS05470 0.51 +0.6 1,322,197 + CA265_RS05470 0.51 -0.2 1,322,214 - CA265_RS05470 0.52 -0.2 1,322,267 + CA265_RS05470 0.54 +1.1 1,322,268 - CA265_RS05470 0.54 -0.1 1,322,306 + CA265_RS05470 0.55 -1.1 1,322,333 + CA265_RS05470 0.56 +0.1 1,322,334 - CA265_RS05470 0.56 +0.1 1,322,428 - CA265_RS05470 0.59 -0.9 1,322,433 + CA265_RS05470 0.59 -0.1 1,322,442 - CA265_RS05470 0.59 +0.1 1,322,580 - CA265_RS05470 0.64 +0.6 1,322,754 + CA265_RS05470 0.69 -0.8 1,322,755 - CA265_RS05470 0.69 -0.6 1,322,755 - CA265_RS05470 0.69 -0.1 1,322,756 + CA265_RS05470 0.69 -0.3 1,322,801 - CA265_RS05470 0.71 -0.5 1,322,815 - CA265_RS05470 0.71 +1.1 1,322,815 - CA265_RS05470 0.71 -0.7 1,322,818 - CA265_RS05470 0.71 -0.6 1,322,900 - CA265_RS05470 0.74 -0.0 1,322,938 + CA265_RS05470 0.75 -1.7 1,322,938 + CA265_RS05470 0.75 -0.1 1,323,112 - CA265_RS05470 0.81 -0.2 1,323,127 + CA265_RS05470 0.81 -0.8 1,323,127 + CA265_RS05470 0.81 -0.8 1,323,213 - CA265_RS05470 0.84 +0.6 1,323,242 - CA265_RS05470 0.85 +0.4 1,323,374 - CA265_RS05470 0.89 +0.4 1,323,405 - -0.1 1,323,412 + -0.1 1,323,498 - -1.2 1,323,506 + -0.6 1,323,524 - +0.8 1,323,550 + -0.6 1,323,559 - +0.2 1,323,595 - +1.1 1,323,596 + -0.1 1,323,616 + -1.2 1,323,617 - -0.4 1,323,667 - -1.1 1,323,738 - -0.8 1,323,753 + -0.1 1,323,757 + +1.0 1,323,757 + -0.2 1,323,883 + -0.6 1,323,885 + -0.5 1,323,905 + CA265_RS05475 0.11 -2.2 1,323,928 - CA265_RS05475 0.12 -0.7 1,323,928 - CA265_RS05475 0.12 -0.1 1,323,928 - CA265_RS05475 0.12 -1.0 1,323,929 + CA265_RS05475 0.12 -0.2 1,324,006 + CA265_RS05475 0.17 -0.2 1,324,074 + CA265_RS05475 0.22 +0.3 1,324,094 + CA265_RS05475 0.23 -0.2 1,324,095 - CA265_RS05475 0.23 -0.3 1,324,095 - CA265_RS05475 0.23 +0.4 1,324,189 - CA265_RS05475 0.29 -0.2 1,324,308 - CA265_RS05475 0.37 -1.1 1,324,410 + CA265_RS05475 0.44 -0.1 1,324,411 - CA265_RS05475 0.44 -0.5 1,324,447 - CA265_RS05475 0.46 -0.9 1,324,474 - CA265_RS05475 0.48 -1.0 1,324,480 - CA265_RS05475 0.49 -0.3 1,324,497 + CA265_RS05475 0.50 +0.3 1,324,498 - CA265_RS05475 0.50 +0.5 1,324,524 - CA265_RS05475 0.51 -0.4 1,324,569 - CA265_RS05475 0.54 +0.0 1,324,659 + CA265_RS05475 0.60 +0.1
Or see this region's nucleotide sequence