Experiment: R2A with Nalidixic 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS04035 and CA265_RS04040 are separated by 36 nucleotides CA265_RS04040 and CA265_RS04045 are separated by 134 nucleotides CA265_RS04045 and CA265_RS04050 are separated by 66 nucleotides
CA265_RS04035: CA265_RS04035 - hypothetical protein, at 967,991 to 969,499
_RS04035
CA265_RS04040: CA265_RS04040 - hypothetical protein, at 969,536 to 969,853
_RS04040
CA265_RS04045: CA265_RS04045 - hypothetical protein, at 969,988 to 970,380
_RS04045
CA265_RS04050: CA265_RS04050 - nucleoside-diphosphate sugar epimerase, at 970,447 to 971,322
_RS04050
Position (kb)
969
970
971 Strain fitness (log2 ratio)
-1
0
1 at 969.097 kb on + strand, within CA265_RS04035 at 969.098 kb on - strand, within CA265_RS04035 at 969.299 kb on + strand, within CA265_RS04035 at 969.369 kb on - strand at 969.374 kb on - strand at 969.479 kb on - strand at 969.543 kb on + strand at 969.584 kb on - strand, within CA265_RS04040 at 969.595 kb on + strand, within CA265_RS04040 at 969.596 kb on - strand, within CA265_RS04040 at 969.596 kb on - strand, within CA265_RS04040 at 969.600 kb on - strand, within CA265_RS04040 at 969.892 kb on - strand at 969.927 kb on + strand at 969.928 kb on - strand at 969.944 kb on - strand at 969.970 kb on - strand at 969.970 kb on - strand at 970.005 kb on + strand at 970.023 kb on - strand at 970.105 kb on + strand, within CA265_RS04045 at 970.106 kb on - strand, within CA265_RS04045 at 970.183 kb on - strand, within CA265_RS04045 at 970.216 kb on + strand, within CA265_RS04045 at 970.230 kb on - strand, within CA265_RS04045 at 970.256 kb on + strand, within CA265_RS04045 at 970.274 kb on + strand, within CA265_RS04045 at 970.275 kb on - strand, within CA265_RS04045 at 970.304 kb on + strand, within CA265_RS04045 at 970.378 kb on + strand at 970.378 kb on + strand at 970.379 kb on - strand at 970.396 kb on + strand at 970.780 kb on + strand, within CA265_RS04050 at 970.781 kb on - strand, within CA265_RS04050 at 970.800 kb on + strand, within CA265_RS04050 at 970.801 kb on - strand, within CA265_RS04050 at 970.801 kb on - strand, within CA265_RS04050 at 970.811 kb on - strand, within CA265_RS04050 at 970.852 kb on - strand, within CA265_RS04050 at 970.886 kb on + strand, within CA265_RS04050 at 970.887 kb on - strand, within CA265_RS04050 at 970.949 kb on + strand, within CA265_RS04050 at 970.960 kb on + strand, within CA265_RS04050 at 970.960 kb on + strand, within CA265_RS04050 at 970.978 kb on - strand, within CA265_RS04050 at 971.049 kb on - strand, within CA265_RS04050 at 971.088 kb on + strand, within CA265_RS04050 at 971.137 kb on - strand, within CA265_RS04050 at 971.203 kb on - strand, within CA265_RS04050 at 971.310 kb on + strand at 971.315 kb on + strand at 971.316 kb on - strand at 971.320 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nalidixic 0.008 mg/ml remove 969,097 + CA265_RS04035 0.73 -0.9 969,098 - CA265_RS04035 0.73 -0.2 969,299 + CA265_RS04035 0.87 -1.1 969,369 - +0.3 969,374 - +0.2 969,479 - -0.1 969,543 + -1.4 969,584 - CA265_RS04040 0.15 +0.1 969,595 + CA265_RS04040 0.19 -0.0 969,596 - CA265_RS04040 0.19 +1.0 969,596 - CA265_RS04040 0.19 +0.4 969,600 - CA265_RS04040 0.20 -0.6 969,892 - -0.1 969,927 + -0.7 969,928 - -0.6 969,944 - -0.1 969,970 - -0.0 969,970 - -0.7 970,005 + +1.2 970,023 - -0.4 970,105 + CA265_RS04045 0.30 -0.3 970,106 - CA265_RS04045 0.30 -0.3 970,183 - CA265_RS04045 0.50 -1.1 970,216 + CA265_RS04045 0.58 -0.7 970,230 - CA265_RS04045 0.62 -1.1 970,256 + CA265_RS04045 0.68 -0.6 970,274 + CA265_RS04045 0.73 -1.6 970,275 - CA265_RS04045 0.73 -0.4 970,304 + CA265_RS04045 0.80 +0.1 970,378 + -1.2 970,378 + -0.8 970,379 - -0.5 970,396 + -1.4 970,780 + CA265_RS04050 0.38 -1.4 970,781 - CA265_RS04050 0.38 -0.0 970,800 + CA265_RS04050 0.40 -0.7 970,801 - CA265_RS04050 0.40 -1.2 970,801 - CA265_RS04050 0.40 -1.0 970,811 - CA265_RS04050 0.42 +0.3 970,852 - CA265_RS04050 0.46 +1.4 970,886 + CA265_RS04050 0.50 -1.0 970,887 - CA265_RS04050 0.50 -0.4 970,949 + CA265_RS04050 0.57 +0.2 970,960 + CA265_RS04050 0.59 -1.3 970,960 + CA265_RS04050 0.59 -0.1 970,978 - CA265_RS04050 0.61 -0.7 971,049 - CA265_RS04050 0.69 -0.1 971,088 + CA265_RS04050 0.73 -0.7 971,137 - CA265_RS04050 0.79 +0.4 971,203 - CA265_RS04050 0.86 -0.1 971,310 + +0.2 971,315 + -0.2 971,316 - -0.0 971,320 - -0.3
Or see this region's nucleotide sequence