Experiment: CDM1 without Glutamine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS12430 and BBR_RS12435 are separated by 603 nucleotides BBR_RS12435 and BBR_RS12440 are separated by 121 nucleotides
BBR_RS12430: BBR_RS12430 - glucose-6-phosphate isomerase, at 535,829 to 537,529
_RS12430
BBR_RS12435: BBR_RS12435 - ABC transporter ATP-binding protein, at 538,133 to 539,551
_RS12435
BBR_RS12440: BBR_RS12440 - Permease of ABC transporter system, at 539,673 to 541,463
_RS12440
Position (kb) 538
539
540 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 537.575 kb on + strand at 537.580 kb on + strand at 537.593 kb on - strand at 537.641 kb on + strand at 537.649 kb on - strand at 537.649 kb on - strand at 537.772 kb on - strand at 537.800 kb on + strand at 537.859 kb on + strand at 537.859 kb on + strand at 537.866 kb on - strand at 537.879 kb on - strand at 537.903 kb on + strand at 537.910 kb on - strand at 537.932 kb on + strand at 537.951 kb on + strand at 537.963 kb on + strand at 537.971 kb on - strand at 537.971 kb on - strand at 538.027 kb on + strand at 538.027 kb on + strand at 538.030 kb on - strand at 538.035 kb on - strand at 538.035 kb on - strand at 538.045 kb on - strand at 538.063 kb on + strand at 538.063 kb on + strand at 538.082 kb on - strand at 538.082 kb on - strand at 538.105 kb on - strand at 538.140 kb on + strand at 538.148 kb on - strand at 538.148 kb on - strand at 538.214 kb on + strand at 538.216 kb on - strand at 538.226 kb on + strand at 538.232 kb on + strand at 538.234 kb on + strand at 538.257 kb on - strand at 538.292 kb on + strand, within BBR_RS12435 at 538.292 kb on + strand, within BBR_RS12435 at 538.313 kb on - strand, within BBR_RS12435 at 538.362 kb on - strand, within BBR_RS12435 at 538.370 kb on + strand, within BBR_RS12435 at 538.371 kb on + strand, within BBR_RS12435 at 538.393 kb on + strand, within BBR_RS12435 at 538.403 kb on - strand, within BBR_RS12435 at 538.435 kb on + strand, within BBR_RS12435 at 538.438 kb on - strand, within BBR_RS12435 at 538.438 kb on - strand, within BBR_RS12435 at 538.466 kb on + strand, within BBR_RS12435 at 538.469 kb on - strand, within BBR_RS12435 at 538.487 kb on - strand, within BBR_RS12435 at 538.487 kb on - strand, within BBR_RS12435 at 538.531 kb on + strand, within BBR_RS12435 at 538.548 kb on - strand, within BBR_RS12435 at 538.548 kb on - strand, within BBR_RS12435 at 538.548 kb on - strand, within BBR_RS12435 at 538.548 kb on - strand, within BBR_RS12435 at 538.574 kb on + strand, within BBR_RS12435 at 538.630 kb on + strand, within BBR_RS12435 at 538.639 kb on - strand, within BBR_RS12435 at 538.645 kb on - strand, within BBR_RS12435 at 538.666 kb on - strand, within BBR_RS12435 at 538.708 kb on + strand, within BBR_RS12435 at 538.738 kb on + strand, within BBR_RS12435 at 538.751 kb on + strand, within BBR_RS12435 at 538.878 kb on - strand, within BBR_RS12435 at 538.878 kb on - strand, within BBR_RS12435 at 538.900 kb on + strand, within BBR_RS12435 at 538.900 kb on + strand, within BBR_RS12435 at 538.900 kb on + strand, within BBR_RS12435 at 538.900 kb on + strand, within BBR_RS12435 at 538.900 kb on + strand, within BBR_RS12435 at 538.906 kb on - strand, within BBR_RS12435 at 538.908 kb on - strand, within BBR_RS12435 at 538.908 kb on - strand, within BBR_RS12435 at 538.908 kb on - strand, within BBR_RS12435 at 538.908 kb on - strand, within BBR_RS12435 at 538.920 kb on - strand, within BBR_RS12435 at 538.937 kb on - strand, within BBR_RS12435 at 538.953 kb on - strand, within BBR_RS12435 at 538.991 kb on + strand, within BBR_RS12435 at 539.020 kb on - strand, within BBR_RS12435 at 539.022 kb on + strand, within BBR_RS12435 at 539.022 kb on + strand, within BBR_RS12435 at 539.022 kb on + strand, within BBR_RS12435 at 539.023 kb on - strand, within BBR_RS12435 at 539.023 kb on - strand, within BBR_RS12435 at 539.030 kb on - strand, within BBR_RS12435 at 539.042 kb on + strand, within BBR_RS12435 at 539.047 kb on + strand, within BBR_RS12435 at 539.064 kb on + strand, within BBR_RS12435 at 539.093 kb on - strand, within BBR_RS12435 at 539.121 kb on - strand, within BBR_RS12435 at 539.191 kb on + strand, within BBR_RS12435 at 539.200 kb on - strand, within BBR_RS12435 at 539.213 kb on + strand, within BBR_RS12435 at 539.213 kb on + strand, within BBR_RS12435 at 539.213 kb on + strand, within BBR_RS12435 at 539.213 kb on + strand, within BBR_RS12435 at 539.213 kb on + strand, within BBR_RS12435 at 539.213 kb on + strand, within BBR_RS12435 at 539.213 kb on + strand, within BBR_RS12435 at 539.213 kb on + strand, within BBR_RS12435 at 539.213 kb on + strand, within BBR_RS12435 at 539.221 kb on - strand, within BBR_RS12435 at 539.221 kb on - strand, within BBR_RS12435 at 539.221 kb on - strand, within BBR_RS12435 at 539.221 kb on - strand, within BBR_RS12435 at 539.221 kb on - strand, within BBR_RS12435 at 539.221 kb on - strand, within BBR_RS12435 at 539.239 kb on + strand, within BBR_RS12435 at 539.254 kb on - strand, within BBR_RS12435 at 539.282 kb on + strand, within BBR_RS12435 at 539.282 kb on + strand, within BBR_RS12435 at 539.290 kb on - strand, within BBR_RS12435 at 539.290 kb on - strand, within BBR_RS12435 at 539.300 kb on + strand, within BBR_RS12435 at 539.327 kb on + strand, within BBR_RS12435 at 539.330 kb on - strand, within BBR_RS12435 at 539.330 kb on - strand, within BBR_RS12435 at 539.330 kb on - strand, within BBR_RS12435 at 539.330 kb on - strand, within BBR_RS12435 at 539.331 kb on + strand, within BBR_RS12435 at 539.336 kb on + strand, within BBR_RS12435 at 539.339 kb on - strand, within BBR_RS12435 at 539.339 kb on - strand, within BBR_RS12435 at 539.412 kb on - strand at 539.477 kb on - strand at 539.485 kb on - strand at 539.508 kb on + strand at 539.545 kb on + strand at 539.554 kb on - strand at 539.555 kb on - strand at 539.561 kb on - strand at 539.566 kb on - strand at 539.619 kb on + strand at 539.624 kb on + strand at 539.626 kb on - strand at 539.647 kb on + strand at 539.650 kb on + strand at 539.655 kb on - strand at 539.660 kb on + strand at 539.671 kb on + strand at 539.689 kb on - strand at 539.696 kb on + strand at 539.696 kb on + strand at 539.709 kb on - strand at 539.783 kb on - strand at 539.785 kb on + strand at 539.833 kb on - strand at 539.849 kb on + strand at 539.857 kb on - strand, within BBR_RS12440 at 539.873 kb on + strand, within BBR_RS12440 at 539.911 kb on - strand, within BBR_RS12440 at 539.911 kb on - strand, within BBR_RS12440 at 539.959 kb on - strand, within BBR_RS12440 at 539.986 kb on - strand, within BBR_RS12440 at 540.045 kb on - strand, within BBR_RS12440 at 540.047 kb on + strand, within BBR_RS12440 at 540.049 kb on + strand, within BBR_RS12440 at 540.049 kb on + strand, within BBR_RS12440 at 540.051 kb on + strand, within BBR_RS12440 at 540.055 kb on - strand, within BBR_RS12440 at 540.055 kb on - strand, within BBR_RS12440 at 540.055 kb on - strand, within BBR_RS12440 at 540.055 kb on - strand, within BBR_RS12440 at 540.059 kb on - strand, within BBR_RS12440 at 540.059 kb on - strand, within BBR_RS12440 at 540.075 kb on + strand, within BBR_RS12440 at 540.106 kb on + strand, within BBR_RS12440 at 540.118 kb on + strand, within BBR_RS12440 at 540.121 kb on - strand, within BBR_RS12440 at 540.121 kb on - strand, within BBR_RS12440 at 540.125 kb on + strand, within BBR_RS12440 at 540.141 kb on + strand, within BBR_RS12440 at 540.170 kb on - strand, within BBR_RS12440 at 540.216 kb on + strand, within BBR_RS12440 at 540.269 kb on - strand, within BBR_RS12440 at 540.279 kb on - strand, within BBR_RS12440 at 540.279 kb on - strand, within BBR_RS12440 at 540.290 kb on - strand, within BBR_RS12440 at 540.299 kb on - strand, within BBR_RS12440 at 540.304 kb on + strand, within BBR_RS12440 at 540.307 kb on + strand, within BBR_RS12440 at 540.307 kb on + strand, within BBR_RS12440 at 540.341 kb on + strand, within BBR_RS12440 at 540.412 kb on - strand, within BBR_RS12440 at 540.443 kb on - strand, within BBR_RS12440 at 540.450 kb on - strand, within BBR_RS12440 at 540.473 kb on + strand, within BBR_RS12440 at 540.477 kb on - strand, within BBR_RS12440 at 540.491 kb on - strand, within BBR_RS12440 at 540.510 kb on - strand, within BBR_RS12440 at 540.532 kb on + strand, within BBR_RS12440 at 540.536 kb on - strand, within BBR_RS12440 at 540.547 kb on + strand, within BBR_RS12440
Per-strain Table
Position Strand Gene LocusTag Fraction CDM1 without Glutamine remove 537,575 + +0.9 537,580 + +0.7 537,593 - -1.9 537,641 + +0.1 537,649 - -2.7 537,649 - -1.7 537,772 - -0.6 537,800 + -1.0 537,859 + +0.5 537,859 + -0.1 537,866 - +0.1 537,879 - +0.3 537,903 + +0.1 537,910 - -1.0 537,932 + +0.3 537,951 + +0.4 537,963 + +0.2 537,971 - +0.5 537,971 - +0.2 538,027 + +0.3 538,027 + +0.6 538,030 - -1.6 538,035 - -0.5 538,035 - -0.5 538,045 - +0.5 538,063 + +0.5 538,063 + -2.4 538,082 - +1.1 538,082 - +0.0 538,105 - +1.3 538,140 + +0.3 538,148 - +0.9 538,148 - -0.1 538,214 + +1.2 538,216 - +0.9 538,226 + +1.4 538,232 + +0.9 538,234 + +0.5 538,257 - +0.4 538,292 + BBR_RS12435 0.11 +0.8 538,292 + BBR_RS12435 0.11 +0.9 538,313 - BBR_RS12435 0.13 -0.9 538,362 - BBR_RS12435 0.16 +1.2 538,370 + BBR_RS12435 0.17 +0.3 538,371 + BBR_RS12435 0.17 -1.1 538,393 + BBR_RS12435 0.18 -1.6 538,403 - BBR_RS12435 0.19 -1.3 538,435 + BBR_RS12435 0.21 +1.1 538,438 - BBR_RS12435 0.21 -0.1 538,438 - BBR_RS12435 0.21 +1.1 538,466 + BBR_RS12435 0.23 +1.1 538,469 - BBR_RS12435 0.24 +0.2 538,487 - BBR_RS12435 0.25 +0.6 538,487 - BBR_RS12435 0.25 +0.6 538,531 + BBR_RS12435 0.28 +0.7 538,548 - BBR_RS12435 0.29 +0.7 538,548 - BBR_RS12435 0.29 +1.0 538,548 - BBR_RS12435 0.29 +0.7 538,548 - BBR_RS12435 0.29 +0.2 538,574 + BBR_RS12435 0.31 +0.9 538,630 + BBR_RS12435 0.35 -1.1 538,639 - BBR_RS12435 0.36 +2.3 538,645 - BBR_RS12435 0.36 +0.7 538,666 - BBR_RS12435 0.38 +0.3 538,708 + BBR_RS12435 0.41 +0.8 538,738 + BBR_RS12435 0.43 -0.1 538,751 + BBR_RS12435 0.44 -1.1 538,878 - BBR_RS12435 0.53 +1.2 538,878 - BBR_RS12435 0.53 +1.2 538,900 + BBR_RS12435 0.54 +0.1 538,900 + BBR_RS12435 0.54 -0.5 538,900 + BBR_RS12435 0.54 +0.5 538,900 + BBR_RS12435 0.54 -0.3 538,900 + BBR_RS12435 0.54 +1.3 538,906 - BBR_RS12435 0.54 -0.3 538,908 - BBR_RS12435 0.55 +1.9 538,908 - BBR_RS12435 0.55 +0.7 538,908 - BBR_RS12435 0.55 +2.1 538,908 - BBR_RS12435 0.55 -0.1 538,920 - BBR_RS12435 0.55 +0.7 538,937 - BBR_RS12435 0.57 -2.9 538,953 - BBR_RS12435 0.58 +0.5 538,991 + BBR_RS12435 0.60 -0.4 539,020 - BBR_RS12435 0.63 +0.3 539,022 + BBR_RS12435 0.63 +0.1 539,022 + BBR_RS12435 0.63 +2.4 539,022 + BBR_RS12435 0.63 +1.3 539,023 - BBR_RS12435 0.63 -0.6 539,023 - BBR_RS12435 0.63 +0.7 539,030 - BBR_RS12435 0.63 +0.1 539,042 + BBR_RS12435 0.64 +0.1 539,047 + BBR_RS12435 0.64 -3.1 539,064 + BBR_RS12435 0.66 +0.3 539,093 - BBR_RS12435 0.68 +0.6 539,121 - BBR_RS12435 0.70 +1.2 539,191 + BBR_RS12435 0.75 +1.1 539,200 - BBR_RS12435 0.75 +0.8 539,213 + BBR_RS12435 0.76 +0.7 539,213 + BBR_RS12435 0.76 +1.4 539,213 + BBR_RS12435 0.76 +0.7 539,213 + BBR_RS12435 0.76 +2.4 539,213 + BBR_RS12435 0.76 +1.4 539,213 + BBR_RS12435 0.76 +0.4 539,213 + BBR_RS12435 0.76 +0.8 539,213 + BBR_RS12435 0.76 -0.1 539,213 + BBR_RS12435 0.76 +0.6 539,221 - BBR_RS12435 0.77 +1.1 539,221 - BBR_RS12435 0.77 +0.1 539,221 - BBR_RS12435 0.77 -0.4 539,221 - BBR_RS12435 0.77 +0.8 539,221 - BBR_RS12435 0.77 +0.8 539,221 - BBR_RS12435 0.77 +1.5 539,239 + BBR_RS12435 0.78 +0.8 539,254 - BBR_RS12435 0.79 +0.8 539,282 + BBR_RS12435 0.81 +0.4 539,282 + BBR_RS12435 0.81 +0.2 539,290 - BBR_RS12435 0.82 +0.8 539,290 - BBR_RS12435 0.82 +0.1 539,300 + BBR_RS12435 0.82 +1.3 539,327 + BBR_RS12435 0.84 -0.0 539,330 - BBR_RS12435 0.84 +1.0 539,330 - BBR_RS12435 0.84 +0.6 539,330 - BBR_RS12435 0.84 +1.7 539,330 - BBR_RS12435 0.84 +1.4 539,331 + BBR_RS12435 0.84 +0.6 539,336 + BBR_RS12435 0.85 -1.0 539,339 - BBR_RS12435 0.85 +0.6 539,339 - BBR_RS12435 0.85 +1.6 539,412 - -0.2 539,477 - +0.9 539,485 - +0.2 539,508 + +0.9 539,545 + +0.9 539,554 - -0.6 539,555 - +0.4 539,561 - -0.7 539,566 - -0.3 539,619 + +0.4 539,624 + -1.3 539,626 - +0.4 539,647 + +0.0 539,650 + -0.9 539,655 - -1.6 539,660 + +0.2 539,671 + -0.3 539,689 - -0.4 539,696 + +0.3 539,696 + -0.7 539,709 - -0.1 539,783 - -0.5 539,785 + -0.3 539,833 - -1.1 539,849 + +0.4 539,857 - BBR_RS12440 0.10 -1.7 539,873 + BBR_RS12440 0.11 +0.7 539,911 - BBR_RS12440 0.13 -0.4 539,911 - BBR_RS12440 0.13 +0.4 539,959 - BBR_RS12440 0.16 -0.1 539,986 - BBR_RS12440 0.17 -0.1 540,045 - BBR_RS12440 0.21 +1.8 540,047 + BBR_RS12440 0.21 +0.3 540,049 + BBR_RS12440 0.21 +0.2 540,049 + BBR_RS12440 0.21 -0.1 540,051 + BBR_RS12440 0.21 -0.1 540,055 - BBR_RS12440 0.21 +0.1 540,055 - BBR_RS12440 0.21 +0.1 540,055 - BBR_RS12440 0.21 -0.4 540,055 - BBR_RS12440 0.21 +0.4 540,059 - BBR_RS12440 0.22 +0.4 540,059 - BBR_RS12440 0.22 -1.1 540,075 + BBR_RS12440 0.22 -0.6 540,106 + BBR_RS12440 0.24 +0.2 540,118 + BBR_RS12440 0.25 -0.0 540,121 - BBR_RS12440 0.25 +0.4 540,121 - BBR_RS12440 0.25 -0.6 540,125 + BBR_RS12440 0.25 +0.2 540,141 + BBR_RS12440 0.26 +0.3 540,170 - BBR_RS12440 0.28 +0.9 540,216 + BBR_RS12440 0.30 +1.0 540,269 - BBR_RS12440 0.33 -0.7 540,279 - BBR_RS12440 0.34 -1.6 540,279 - BBR_RS12440 0.34 -2.7 540,290 - BBR_RS12440 0.34 -0.4 540,299 - BBR_RS12440 0.35 -0.1 540,304 + BBR_RS12440 0.35 +1.0 540,307 + BBR_RS12440 0.35 -0.3 540,307 + BBR_RS12440 0.35 +0.2 540,341 + BBR_RS12440 0.37 +0.3 540,412 - BBR_RS12440 0.41 -1.0 540,443 - BBR_RS12440 0.43 -0.1 540,450 - BBR_RS12440 0.43 +0.2 540,473 + BBR_RS12440 0.45 +1.1 540,477 - BBR_RS12440 0.45 -1.6 540,491 - BBR_RS12440 0.46 -1.5 540,510 - BBR_RS12440 0.47 -0.1 540,532 + BBR_RS12440 0.48 +1.7 540,536 - BBR_RS12440 0.48 -1.4 540,547 + BBR_RS12440 0.49 +0.5
Or see this region's nucleotide sequence