Experiment: MoYLS4 with Polymyxin B sulfate 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_1440 and DvMF_1441 are separated by 124 nucleotides DvMF_1441 and rpmE are separated by 421 nucleotides rpmE and DvMF_1443 are separated by 112 nucleotides
DvMF_1440: DvMF_1440 - glyceraldehyde-3-phosphate dehydrogenase, type I (RefSeq), at 1,761,740 to 1,762,756
_1440
DvMF_1441: DvMF_1441 - hemolysin A (RefSeq), at 1,762,881 to 1,763,711
_1441
DvMF_1442: rpmE - 50S ribosomal protein L31 (RefSeq), at 1,764,133 to 1,764,348
rpmE
DvMF_1443: DvMF_1443 - protein of unknown function DUF1385 (RefSeq), at 1,764,461 to 1,765,540
_1443
Position (kb)
1762
1763
1764 Strain fitness (log2 ratio)
-1
0
1 at 1761.970 kb on - strand, within DvMF_1440 at 1762.063 kb on - strand, within DvMF_1440 at 1762.248 kb on - strand, within DvMF_1440 at 1762.258 kb on + strand, within DvMF_1440 at 1762.258 kb on + strand, within DvMF_1440 at 1762.266 kb on - strand, within DvMF_1440 at 1762.336 kb on - strand, within DvMF_1440 at 1762.428 kb on - strand, within DvMF_1440 at 1762.594 kb on + strand, within DvMF_1440 at 1762.869 kb on + strand at 1762.961 kb on - strand at 1763.293 kb on + strand, within DvMF_1441 at 1763.293 kb on + strand, within DvMF_1441 at 1763.301 kb on - strand, within DvMF_1441 at 1763.313 kb on + strand, within DvMF_1441 at 1763.572 kb on + strand, within DvMF_1441 at 1763.707 kb on - strand at 1764.053 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Polymyxin B sulfate 0.1 mg/ml remove 1,761,970 - DvMF_1440 0.23 +0.4 1,762,063 - DvMF_1440 0.32 +0.5 1,762,248 - DvMF_1440 0.50 +0.4 1,762,258 + DvMF_1440 0.51 +0.7 1,762,258 + DvMF_1440 0.51 +0.4 1,762,266 - DvMF_1440 0.52 -0.0 1,762,336 - DvMF_1440 0.59 +0.6 1,762,428 - DvMF_1440 0.68 +0.4 1,762,594 + DvMF_1440 0.84 -0.4 1,762,869 + -0.1 1,762,961 - +0.5 1,763,293 + DvMF_1441 0.50 +0.9 1,763,293 + DvMF_1441 0.50 +0.6 1,763,301 - DvMF_1441 0.51 +0.3 1,763,313 + DvMF_1441 0.52 +1.0 1,763,572 + DvMF_1441 0.83 +0.4 1,763,707 - +0.3 1,764,053 - -0.1
Or see this region's nucleotide sequence