Experiment: MoYLS4 with Polymyxin B sulfate 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_1217 and DvMF_1218 are separated by 74 nucleotides DvMF_1218 and DvMF_1219 are separated by 603 nucleotides DvMF_1219 and DvMF_1220 are separated by 122 nucleotides
DvMF_1217: DvMF_1217 - formate dehydrogenase, alpha subunit (RefSeq), at 1,498,036 to 1,501,062
_1217
DvMF_1218: DvMF_1218 - hypothetical protein (RefSeq), at 1,501,137 to 1,501,373
_1218
DvMF_1219: DvMF_1219 - transposase, putative (RefSeq), at 1,501,977 to 1,502,900
_1219
DvMF_1220: DvMF_1220 - alkyl hydroperoxide reductase/ Thiol specific antioxidant/ Mal allergen (RefSeq), at 1,503,023 to 1,503,805
_1220
Position (kb)
1501
1502
1503 Strain fitness (log2 ratio)
-1
0
1 at 1501.534 kb on - strand at 1501.725 kb on - strand at 1501.853 kb on + strand at 1501.853 kb on + strand at 1502.790 kb on - strand, within DvMF_1219 at 1502.841 kb on + strand at 1502.921 kb on - strand at 1503.002 kb on - strand at 1503.109 kb on + strand, within DvMF_1220 at 1503.136 kb on + strand, within DvMF_1220 at 1503.207 kb on + strand, within DvMF_1220 at 1503.406 kb on - strand, within DvMF_1220 at 1503.485 kb on - strand, within DvMF_1220 at 1503.623 kb on - strand, within DvMF_1220 at 1503.813 kb on + strand at 1503.813 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Polymyxin B sulfate 0.1 mg/ml remove 1,501,534 - -0.6 1,501,725 - +0.0 1,501,853 + -0.4 1,501,853 + -0.2 1,502,790 - DvMF_1219 0.88 +0.0 1,502,841 + -0.8 1,502,921 - -1.2 1,503,002 - -0.4 1,503,109 + DvMF_1220 0.11 -1.1 1,503,136 + DvMF_1220 0.14 -0.5 1,503,207 + DvMF_1220 0.23 -0.7 1,503,406 - DvMF_1220 0.49 -0.3 1,503,485 - DvMF_1220 0.59 -0.6 1,503,623 - DvMF_1220 0.77 -1.0 1,503,813 + -0.8 1,503,813 + +0.3
Or see this region's nucleotide sequence