Experiment: MoYLS4 with Polymyxin B sulfate 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_1132 and DvMF_1133 are separated by 106 nucleotides DvMF_1133 and DvMF_1134 are separated by 182 nucleotides DvMF_1134 and DvMF_1135 are separated by 375 nucleotides
DvMF_1132: DvMF_1132 - pseudouridine synthase (RefSeq), at 1,389,000 to 1,390,391
_1132
DvMF_1133: DvMF_1133 - hypothetical protein (RefSeq), at 1,390,498 to 1,391,184
_1133
DvMF_1134: DvMF_1134 - single-stranded-DNA-specific exonuclease RecJ (RefSeq), at 1,391,367 to 1,393,082
_1134
DvMF_1135: DvMF_1135 - metal dependent phosphohydrolase (RefSeq), at 1,393,458 to 1,394,480
_1135
Position (kb)
1391
1392
1393
1394 Strain fitness (log2 ratio)
-2
-1
0
1 at 1390.558 kb on - strand at 1390.624 kb on - strand, within DvMF_1133 at 1390.695 kb on - strand, within DvMF_1133 at 1390.746 kb on + strand, within DvMF_1133 at 1391.254 kb on + strand at 1391.262 kb on - strand at 1391.327 kb on - strand at 1391.391 kb on + strand at 1391.435 kb on + strand at 1391.437 kb on + strand at 1391.437 kb on + strand at 1391.850 kb on - strand, within DvMF_1134 at 1391.901 kb on + strand, within DvMF_1134 at 1391.911 kb on + strand, within DvMF_1134 at 1392.000 kb on - strand, within DvMF_1134 at 1392.237 kb on - strand, within DvMF_1134 at 1392.500 kb on + strand, within DvMF_1134 at 1392.603 kb on - strand, within DvMF_1134 at 1392.778 kb on + strand, within DvMF_1134 at 1392.778 kb on + strand, within DvMF_1134 at 1392.791 kb on + strand, within DvMF_1134 at 1392.832 kb on + strand, within DvMF_1134 at 1392.936 kb on + strand at 1393.039 kb on - strand at 1393.088 kb on + strand at 1393.154 kb on + strand at 1393.162 kb on - strand at 1393.172 kb on - strand at 1393.172 kb on - strand at 1393.213 kb on + strand at 1393.571 kb on - strand, within DvMF_1135 at 1393.636 kb on + strand, within DvMF_1135
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Polymyxin B sulfate 0.1 mg/ml remove 1,390,558 - -0.5 1,390,624 - DvMF_1133 0.18 -0.3 1,390,695 - DvMF_1133 0.29 -0.4 1,390,746 + DvMF_1133 0.36 -0.6 1,391,254 + -0.9 1,391,262 - -0.1 1,391,327 - +0.3 1,391,391 + -0.0 1,391,435 + -0.8 1,391,437 + -1.0 1,391,437 + -0.9 1,391,850 - DvMF_1134 0.28 -0.8 1,391,901 + DvMF_1134 0.31 -1.0 1,391,911 + DvMF_1134 0.32 -1.1 1,392,000 - DvMF_1134 0.37 -1.0 1,392,237 - DvMF_1134 0.51 -0.9 1,392,500 + DvMF_1134 0.66 -1.8 1,392,603 - DvMF_1134 0.72 -0.8 1,392,778 + DvMF_1134 0.82 -1.4 1,392,778 + DvMF_1134 0.82 -2.0 1,392,791 + DvMF_1134 0.83 -0.2 1,392,832 + DvMF_1134 0.85 -1.5 1,392,936 + -0.6 1,393,039 - -1.2 1,393,088 + -0.3 1,393,154 + -1.5 1,393,162 - -0.0 1,393,172 - -0.6 1,393,172 - -1.4 1,393,213 + -0.5 1,393,571 - DvMF_1135 0.11 -0.1 1,393,636 + DvMF_1135 0.17 -0.3
Or see this region's nucleotide sequence