Experiment: MoYLS4 with Polymyxin B sulfate 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0843 and DvMF_0844 are separated by 424 nucleotides DvMF_0844 and DvMF_0845 are separated by 111 nucleotides DvMF_0845 and DvMF_0846 are separated by 13 nucleotides
DvMF_0843: DvMF_0843 - glycosyl transferase family 9 (RefSeq), at 1,018,796 to 1,020,058
_0843
DvMF_0844: DvMF_0844 - Sua5/YciO/YrdC/YwlC family protein (RefSeq), at 1,020,483 to 1,021,139
_0844
DvMF_0845: DvMF_0845 - protein of unknown function DUF163 (RefSeq), at 1,021,251 to 1,021,718
_0845
DvMF_0846: DvMF_0846 - hypothetical protein (RefSeq), at 1,021,732 to 1,024,080
_0846
Position (kb)
1020
1021
1022 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1019.646 kb on + strand, within DvMF_0843 at 1019.654 kb on - strand, within DvMF_0843 at 1019.669 kb on - strand, within DvMF_0843 at 1019.678 kb on - strand, within DvMF_0843 at 1019.790 kb on - strand, within DvMF_0843 at 1019.800 kb on - strand, within DvMF_0843 at 1019.836 kb on - strand, within DvMF_0843 at 1019.889 kb on + strand, within DvMF_0843 at 1019.892 kb on - strand, within DvMF_0843 at 1020.256 kb on - strand at 1021.210 kb on + strand at 1021.481 kb on + strand, within DvMF_0845 at 1021.489 kb on - strand, within DvMF_0845 at 1021.676 kb on - strand at 1022.071 kb on - strand, within DvMF_0846
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Polymyxin B sulfate 0.1 mg/ml remove 1,019,646 + DvMF_0843 0.67 -2.0 1,019,654 - DvMF_0843 0.68 -1.1 1,019,669 - DvMF_0843 0.69 -2.2 1,019,678 - DvMF_0843 0.70 -0.3 1,019,790 - DvMF_0843 0.79 +0.7 1,019,800 - DvMF_0843 0.79 -0.5 1,019,836 - DvMF_0843 0.82 -0.4 1,019,889 + DvMF_0843 0.87 -0.0 1,019,892 - DvMF_0843 0.87 +0.3 1,020,256 - -0.5 1,021,210 + -0.5 1,021,481 + DvMF_0845 0.49 -0.5 1,021,489 - DvMF_0845 0.51 +0.2 1,021,676 - -0.4 1,022,071 - DvMF_0846 0.14 -4.2
Or see this region's nucleotide sequence