Experiment: MoYLS4 with Polymyxin B sulfate 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0595 and DvMF_0596 overlap by 4 nucleotides DvMF_0596 and DvMF_0597 overlap by 4 nucleotides DvMF_0597 and DvMF_0598 are separated by 260 nucleotides
DvMF_0595: DvMF_0595 - phosphonate ABC transporter, inner membrane subunit (RefSeq), at 706,786 to 707,580
_0595
DvMF_0596: DvMF_0596 - phosphonate ABC transporter, inner membrane subunit (RefSeq), at 707,577 to 708,365
_0596
DvMF_0597: DvMF_0597 - phosphonate ABC transporter, ATPase subunit (RefSeq), at 708,362 to 709,174
_0597
DvMF_0598: DvMF_0598 - phosphonate ABC transporter, periplasmic phosphonate-binding protein (RefSeq), at 709,435 to 710,490
_0598
Position (kb) 708
709
710 Strain fitness (log2 ratio) -1
0
1 at 707.436 kb on + strand, within DvMF_0595 at 707.563 kb on + strand at 707.578 kb on + strand at 707.733 kb on - strand, within DvMF_0596 at 707.895 kb on + strand, within DvMF_0596 at 708.290 kb on - strand at 708.401 kb on - strand at 708.917 kb on - strand, within DvMF_0597 at 708.929 kb on + strand, within DvMF_0597 at 709.020 kb on + strand, within DvMF_0597 at 709.084 kb on - strand, within DvMF_0597 at 709.694 kb on - strand, within DvMF_0598 at 709.874 kb on + strand, within DvMF_0598
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Polymyxin B sulfate 0.1 mg/ml remove 707,436 + DvMF_0595 0.82 +0.9 707,563 + +0.7 707,578 + +0.6 707,733 - DvMF_0596 0.20 +1.4 707,895 + DvMF_0596 0.40 -0.6 708,290 - +0.4 708,401 - +0.1 708,917 - DvMF_0597 0.68 -0.4 708,929 + DvMF_0597 0.70 -0.3 709,020 + DvMF_0597 0.81 -0.8 709,084 - DvMF_0597 0.89 -0.8 709,694 - DvMF_0598 0.25 +1.1 709,874 + DvMF_0598 0.42 -0.0
Or see this region's nucleotide sequence