Experiment: MoYLS4 with Polymyxin B sulfate 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0419 and DvMF_0420 are separated by 222 nucleotides DvMF_0420 and DvMF_0421 are separated by 153 nucleotides DvMF_0421 and DvMF_0422 are separated by 129 nucleotides
DvMF_0419: DvMF_0419 - metal dependent phosphohydrolase (RefSeq), at 494,082 to 495,497
_0419
DvMF_0420: DvMF_0420 - putative manganese-dependent inorganic pyrophosphatase (RefSeq), at 495,720 to 496,640
_0420
DvMF_0421: DvMF_0421 - hypothetical protein (RefSeq), at 496,794 to 497,363
_0421
DvMF_0422: DvMF_0422 - PTS system sorbose subfamily IIB component (RefSeq), at 497,493 to 497,957
_0422
Position (kb) 495
496
497 Strain fitness (log2 ratio) -2
-1
0
1 at 494.739 kb on + strand, within DvMF_0419 at 494.744 kb on + strand, within DvMF_0419 at 494.863 kb on + strand, within DvMF_0419 at 494.868 kb on + strand, within DvMF_0419 at 494.876 kb on - strand, within DvMF_0419 at 495.078 kb on - strand, within DvMF_0419 at 495.079 kb on - strand, within DvMF_0419 at 495.256 kb on - strand, within DvMF_0419 at 495.401 kb on + strand at 495.566 kb on + strand at 496.913 kb on - strand, within DvMF_0421 at 497.089 kb on - strand, within DvMF_0421 at 497.305 kb on + strand, within DvMF_0421 at 497.308 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Polymyxin B sulfate 0.1 mg/ml remove 494,739 + DvMF_0419 0.46 -0.6 494,744 + DvMF_0419 0.47 -0.2 494,863 + DvMF_0419 0.55 +0.1 494,868 + DvMF_0419 0.56 -0.0 494,876 - DvMF_0419 0.56 -0.5 495,078 - DvMF_0419 0.70 +0.6 495,079 - DvMF_0419 0.70 -1.8 495,256 - DvMF_0419 0.83 -0.6 495,401 + -0.6 495,566 + -0.6 496,913 - DvMF_0421 0.21 +0.6 497,089 - DvMF_0421 0.52 +0.8 497,305 + DvMF_0421 0.90 +0.6 497,308 - +0.7
Or see this region's nucleotide sequence