Experiment: MoYLS4 with Polymyxin B sulfate 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0352 and DvMF_0353 are separated by 285 nucleotides DvMF_0353 and DvMF_0354 are separated by 96 nucleotides DvMF_0354 and DvMF_0355 are separated by 16 nucleotides
DvMF_0352: DvMF_0352 - putative phage repressor (RefSeq), at 409,755 to 410,540
_0352
DvMF_0353: DvMF_0353 - diaminopimelate decarboxylase (RefSeq), at 410,826 to 412,076
_0353
DvMF_0354: DvMF_0354 - hypothetical protein (RefSeq), at 412,173 to 412,694
_0354
DvMF_0355: DvMF_0355 - DNA mismatch repair protein MutS (RefSeq), at 412,711 to 415,443
_0355
Position (kb) 410
411
412
413 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 410.550 kb on - strand at 410.876 kb on - strand at 411.149 kb on - strand, within DvMF_0353 at 411.543 kb on + strand, within DvMF_0353 at 411.604 kb on + strand, within DvMF_0353 at 411.683 kb on + strand, within DvMF_0353 at 411.894 kb on + strand, within DvMF_0353 at 411.995 kb on - strand at 412.093 kb on - strand at 412.120 kb on + strand at 412.253 kb on + strand, within DvMF_0354 at 412.260 kb on + strand, within DvMF_0354 at 412.260 kb on + strand, within DvMF_0354 at 412.789 kb on - strand at 412.826 kb on + strand at 412.834 kb on - strand at 412.959 kb on - strand at 412.987 kb on - strand, within DvMF_0355
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Polymyxin B sulfate 0.1 mg/ml remove 410,550 - -0.5 410,876 - -0.5 411,149 - DvMF_0353 0.26 -0.5 411,543 + DvMF_0353 0.57 -0.6 411,604 + DvMF_0353 0.62 -1.5 411,683 + DvMF_0353 0.69 +1.3 411,894 + DvMF_0353 0.85 -0.7 411,995 - -0.6 412,093 - -0.6 412,120 + -1.3 412,253 + DvMF_0354 0.15 -4.6 412,260 + DvMF_0354 0.17 -3.9 412,260 + DvMF_0354 0.17 -3.1 412,789 - -3.2 412,826 + -0.4 412,834 - +0.7 412,959 - +0.2 412,987 - DvMF_0355 0.10 -0.4
Or see this region's nucleotide sequence