Experiment: MoYLS4 with Polymyxin B sulfate 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0320 and DvMF_0321 are separated by 525 nucleotides DvMF_0321 and DvMF_0322 are separated by 279 nucleotides DvMF_0322 and DvMF_0323 overlap by 4 nucleotides
DvMF_0320: DvMF_0320 - Formate dehydrogenase (RefSeq), at 371,427 to 373,529
_0320
DvMF_0321: DvMF_0321 - iron-containing alcohol dehydrogenase (RefSeq), at 374,055 to 375,236
_0321
DvMF_0322: DvMF_0322 - heterodisulfide reductase, C subunit (RefSeq), at 375,516 to 376,076
_0322
DvMF_0323: DvMF_0323 - CoB--CoM heterodisulfide reductase (RefSeq), at 376,073 to 377,053
_0323
Position (kb) 374
375
376 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 373.154 kb on + strand, within DvMF_0320 at 373.162 kb on - strand, within DvMF_0320 at 373.184 kb on + strand, within DvMF_0320 at 373.187 kb on - strand, within DvMF_0320 at 373.238 kb on - strand, within DvMF_0320 at 373.398 kb on + strand at 373.413 kb on - strand at 373.413 kb on - strand at 373.444 kb on + strand at 373.477 kb on - strand at 373.519 kb on - strand at 373.519 kb on - strand at 373.535 kb on + strand at 373.666 kb on - strand at 373.781 kb on - strand at 373.852 kb on - strand at 373.930 kb on + strand at 373.990 kb on - strand at 373.990 kb on - strand at 373.990 kb on - strand at 374.038 kb on - strand at 374.072 kb on + strand at 374.072 kb on + strand at 374.072 kb on + strand at 374.095 kb on + strand at 374.103 kb on - strand at 374.158 kb on + strand at 374.158 kb on + strand at 374.158 kb on + strand at 374.270 kb on - strand, within DvMF_0321 at 374.341 kb on + strand, within DvMF_0321 at 374.657 kb on - strand, within DvMF_0321 at 374.657 kb on - strand, within DvMF_0321 at 374.708 kb on - strand, within DvMF_0321 at 374.720 kb on + strand, within DvMF_0321 at 374.810 kb on + strand, within DvMF_0321 at 374.855 kb on + strand, within DvMF_0321 at 375.058 kb on + strand, within DvMF_0321 at 375.580 kb on + strand, within DvMF_0322 at 375.713 kb on - strand, within DvMF_0322
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Polymyxin B sulfate 0.1 mg/ml remove 373,154 + DvMF_0320 0.82 -0.4 373,162 - DvMF_0320 0.83 +0.1 373,184 + DvMF_0320 0.84 -0.4 373,187 - DvMF_0320 0.84 -0.6 373,238 - DvMF_0320 0.86 +0.1 373,398 + -0.7 373,413 - -0.4 373,413 - -0.5 373,444 + -0.0 373,477 - +0.1 373,519 - -0.6 373,519 - -0.3 373,535 + +0.4 373,666 - -1.5 373,781 - -0.4 373,852 - -0.4 373,930 + -0.2 373,990 - -0.5 373,990 - +0.9 373,990 - -0.5 374,038 - -1.5 374,072 + -1.3 374,072 + -0.3 374,072 + -1.3 374,095 + -3.3 374,103 - +0.3 374,158 + -0.1 374,158 + -0.7 374,158 + -0.7 374,270 - DvMF_0321 0.18 -0.0 374,341 + DvMF_0321 0.24 -0.4 374,657 - DvMF_0321 0.51 -0.7 374,657 - DvMF_0321 0.51 -0.6 374,708 - DvMF_0321 0.55 -0.6 374,720 + DvMF_0321 0.56 -1.0 374,810 + DvMF_0321 0.64 -0.6 374,855 + DvMF_0321 0.68 -0.2 375,058 + DvMF_0321 0.85 -0.4 375,580 + DvMF_0322 0.11 +1.8 375,713 - DvMF_0322 0.35 +1.4
Or see this region's nucleotide sequence