Experiment: succinate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dapD and Shew_2639 are separated by 210 nucleotides Shew_2639 and purU are separated by 108 nucleotides purU and Shew_2641 are separated by 33 nucleotides
Shew_2638: dapD - 2,3,4,5-tetrahydropyridine-2-carboxylate N-succinyltransferase (RefSeq), at 3,114,164 to 3,114,988
dapD
Shew_2639: Shew_2639 - hypothetical protein (RefSeq), at 3,115,199 to 3,115,543
_2639
Shew_2640: purU - formyltetrahydrofolate deformylase (RefSeq), at 3,115,652 to 3,116,485
purU
Shew_2641: Shew_2641 - PTS system, glucose-like IIB subunint (RefSeq), at 3,116,519 to 3,117,991
_2641
Position (kb)
3115
3116 Strain fitness (log2 ratio)
-2
-1
0
1 at 3115.421 kb on + strand, within Shew_2639 at 3115.652 kb on - strand at 3115.713 kb on + strand at 3115.721 kb on - strand at 3115.882 kb on + strand, within purU at 3116.071 kb on - strand, within purU at 3116.127 kb on - strand, within purU at 3116.421 kb on + strand at 3116.421 kb on + strand at 3116.429 kb on - strand at 3116.440 kb on + strand at 3116.448 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction succinate (C) remove 3,115,421 + Shew_2639 0.64 +1.1 3,115,652 - +0.3 3,115,713 + +1.2 3,115,721 - -1.0 3,115,882 + purU Shew_2640 0.28 -0.5 3,116,071 - purU Shew_2640 0.50 -1.0 3,116,127 - purU Shew_2640 0.57 -0.6 3,116,421 + -0.9 3,116,421 + +0.1 3,116,429 - -2.3 3,116,440 + -0.3 3,116,448 - -0.0
Or see this region's nucleotide sequence