Experiment: CDM1 without Tryptophan
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS12895 and BBR_RS12900 are separated by 305 nucleotides BBR_RS12900 and BBR_RS12905 are separated by 5 nucleotides
BBR_RS12895: BBR_RS12895 - quinone-dependent dihydroorotate dehydrogenase, at 644,932 to 646,083
_RS12895
BBR_RS12900: BBR_RS12900 - DeoR/GlpR transcriptional regulator, at 646,389 to 647,192
_RS12900
BBR_RS12905: BBR_RS12905 - galactose-1-phosphate uridylyltransferase, at 647,198 to 648,448
_RS12905
Position (kb) 646
647
648 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 645.411 kb on - strand, within BBR_RS12895 at 645.444 kb on - strand, within BBR_RS12895 at 645.512 kb on + strand, within BBR_RS12895 at 645.539 kb on + strand, within BBR_RS12895 at 645.596 kb on + strand, within BBR_RS12895 at 645.604 kb on - strand, within BBR_RS12895 at 645.609 kb on - strand, within BBR_RS12895 at 645.609 kb on - strand, within BBR_RS12895 at 645.646 kb on + strand, within BBR_RS12895 at 645.698 kb on + strand, within BBR_RS12895 at 645.706 kb on - strand, within BBR_RS12895 at 645.715 kb on + strand, within BBR_RS12895 at 645.723 kb on - strand, within BBR_RS12895 at 645.726 kb on + strand, within BBR_RS12895 at 645.734 kb on - strand, within BBR_RS12895 at 645.770 kb on - strand, within BBR_RS12895 at 645.875 kb on + strand, within BBR_RS12895 at 645.875 kb on + strand, within BBR_RS12895 at 645.875 kb on + strand, within BBR_RS12895 at 645.883 kb on - strand, within BBR_RS12895 at 645.883 kb on - strand, within BBR_RS12895 at 645.883 kb on - strand, within BBR_RS12895 at 645.883 kb on - strand, within BBR_RS12895 at 645.885 kb on - strand, within BBR_RS12895 at 645.907 kb on - strand, within BBR_RS12895 at 645.911 kb on + strand, within BBR_RS12895 at 645.914 kb on - strand, within BBR_RS12895 at 645.927 kb on - strand, within BBR_RS12895 at 646.013 kb on - strand at 646.026 kb on + strand at 646.034 kb on - strand at 646.034 kb on - strand at 646.090 kb on - strand at 646.111 kb on + strand at 646.173 kb on - strand at 646.173 kb on - strand at 646.177 kb on - strand at 646.219 kb on - strand at 646.234 kb on + strand at 646.308 kb on - strand at 646.308 kb on - strand at 646.592 kb on - strand, within BBR_RS12900 at 646.962 kb on + strand, within BBR_RS12900 at 647.041 kb on + strand, within BBR_RS12900 at 647.049 kb on + strand, within BBR_RS12900 at 647.235 kb on + strand at 647.277 kb on + strand at 647.285 kb on - strand at 647.417 kb on + strand, within BBR_RS12905 at 647.765 kb on - strand, within BBR_RS12905 at 647.955 kb on - strand, within BBR_RS12905 at 648.176 kb on - strand, within BBR_RS12905
Per-strain Table
Position Strand Gene LocusTag Fraction CDM1 without Tryptophan remove 645,411 - BBR_RS12895 0.42 -1.5 645,444 - BBR_RS12895 0.44 +1.4 645,512 + BBR_RS12895 0.50 +0.0 645,539 + BBR_RS12895 0.53 -0.6 645,596 + BBR_RS12895 0.58 -0.7 645,604 - BBR_RS12895 0.58 +0.6 645,609 - BBR_RS12895 0.59 -0.3 645,609 - BBR_RS12895 0.59 -0.0 645,646 + BBR_RS12895 0.62 +0.2 645,698 + BBR_RS12895 0.66 -1.0 645,706 - BBR_RS12895 0.67 +0.5 645,715 + BBR_RS12895 0.68 +0.8 645,723 - BBR_RS12895 0.69 +0.9 645,726 + BBR_RS12895 0.69 +0.3 645,734 - BBR_RS12895 0.70 +0.0 645,770 - BBR_RS12895 0.73 +0.8 645,875 + BBR_RS12895 0.82 -2.1 645,875 + BBR_RS12895 0.82 +0.1 645,875 + BBR_RS12895 0.82 +0.1 645,883 - BBR_RS12895 0.83 -0.5 645,883 - BBR_RS12895 0.83 +0.3 645,883 - BBR_RS12895 0.83 -0.2 645,883 - BBR_RS12895 0.83 -0.0 645,885 - BBR_RS12895 0.83 -0.7 645,907 - BBR_RS12895 0.85 -0.1 645,911 + BBR_RS12895 0.85 -0.8 645,914 - BBR_RS12895 0.85 +0.3 645,927 - BBR_RS12895 0.86 -0.9 646,013 - -0.4 646,026 + -0.0 646,034 - +0.1 646,034 - -0.5 646,090 - -0.7 646,111 + -2.1 646,173 - -0.8 646,173 - -1.1 646,177 - +0.5 646,219 - -0.6 646,234 + +0.1 646,308 - -1.6 646,308 - -0.4 646,592 - BBR_RS12900 0.25 -1.4 646,962 + BBR_RS12900 0.71 -2.7 647,041 + BBR_RS12900 0.81 -4.1 647,049 + BBR_RS12900 0.82 -3.4 647,235 + -1.4 647,277 + -2.7 647,285 - -2.5 647,417 + BBR_RS12905 0.18 -1.8 647,765 - BBR_RS12905 0.45 -1.1 647,955 - BBR_RS12905 0.61 -1.0 648,176 - BBR_RS12905 0.78 -1.7
Or see this region's nucleotide sequence