Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_1818 and Ac3H11_1819 are separated by 29 nucleotides Ac3H11_1819 and Ac3H11_1820 are separated by 15 nucleotides Ac3H11_1820 and Ac3H11_1821 are separated by 49 nucleotides Ac3H11_1821 and Ac3H11_1822 are separated by 33 nucleotides Ac3H11_1822 and Ac3H11_1823 are separated by 12 nucleotides
Ac3H11_1818 - Dihydroneopterin aldolase (EC 4.1.2.25), at 23,274 to 23,648
_1818
Ac3H11_1819 - FolM Alternative dihydrofolate reductase 1, at 23,678 to 24,463
_1819
Ac3H11_1820 - COG1565: Uncharacterized conserved protein, at 24,479 to 25,597
_1820
Ac3H11_1821 - hypothetical protein, at 25,647 to 25,805
_1821
Ac3H11_1822 - Thioredoxin, at 25,839 to 26,306
_1822
Ac3H11_1823 - INTEGRAL MEMBRANE PROTEIN (Rhomboid family), at 26,319 to 26,792
_1823
Position (kb) 24
25
26 Strain fitness (log2 ratio) -2
-1
0
1
2 at 23.973 kb on + strand, within Ac3H11_1819 at 23.974 kb on - strand, within Ac3H11_1819 at 24.318 kb on - strand, within Ac3H11_1819 at 24.318 kb on - strand, within Ac3H11_1819 at 24.457 kb on + strand at 24.457 kb on + strand at 24.458 kb on - strand at 24.458 kb on - strand at 24.458 kb on - strand at 24.458 kb on - strand at 25.645 kb on + strand at 25.645 kb on + strand at 25.646 kb on - strand at 25.646 kb on - strand at 25.803 kb on + strand at 25.803 kb on + strand at 25.803 kb on + strand at 25.804 kb on - strand at 25.980 kb on - strand, within Ac3H11_1822 at 26.187 kb on + strand, within Ac3H11_1822 at 26.187 kb on + strand, within Ac3H11_1822 at 26.187 kb on + strand, within Ac3H11_1822 at 26.187 kb on + strand, within Ac3H11_1822 at 26.187 kb on + strand, within Ac3H11_1822 at 26.304 kb on + strand at 26.305 kb on - strand at 26.377 kb on + strand, within Ac3H11_1823 at 26.377 kb on + strand, within Ac3H11_1823 at 26.377 kb on + strand, within Ac3H11_1823 at 26.378 kb on - strand, within Ac3H11_1823 at 26.378 kb on - strand, within Ac3H11_1823 at 26.378 kb on - strand, within Ac3H11_1823 at 26.425 kb on + strand, within Ac3H11_1823 at 26.575 kb on + strand, within Ac3H11_1823 at 26.575 kb on + strand, within Ac3H11_1823 at 26.575 kb on + strand, within Ac3H11_1823 at 26.575 kb on + strand, within Ac3H11_1823 at 26.575 kb on + strand, within Ac3H11_1823 at 26.576 kb on - strand, within Ac3H11_1823 at 26.596 kb on + strand, within Ac3H11_1823 at 26.596 kb on + strand, within Ac3H11_1823 at 26.596 kb on + strand, within Ac3H11_1823 at 26.597 kb on - strand, within Ac3H11_1823
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 23,973 + Ac3H11_1819 0.38 -1.1 23,974 - Ac3H11_1819 0.38 -2.0 24,318 - Ac3H11_1819 0.81 +0.4 24,318 - Ac3H11_1819 0.81 +0.1 24,457 + -1.9 24,457 + -1.8 24,458 - +0.0 24,458 - -0.3 24,458 - +0.3 24,458 - +0.5 25,645 + +1.0 25,645 + -0.3 25,646 - +0.3 25,646 - -0.1 25,803 + +0.1 25,803 + -0.1 25,803 + +0.3 25,804 - +0.1 25,980 - Ac3H11_1822 0.30 +0.4 26,187 + Ac3H11_1822 0.74 +0.1 26,187 + Ac3H11_1822 0.74 +0.0 26,187 + Ac3H11_1822 0.74 -0.1 26,187 + Ac3H11_1822 0.74 +1.1 26,187 + Ac3H11_1822 0.74 -0.1 26,304 + +0.1 26,305 - +0.6 26,377 + Ac3H11_1823 0.12 +1.2 26,377 + Ac3H11_1823 0.12 -0.9 26,377 + Ac3H11_1823 0.12 -0.1 26,378 - Ac3H11_1823 0.12 +1.8 26,378 - Ac3H11_1823 0.12 -0.1 26,378 - Ac3H11_1823 0.12 +0.2 26,425 + Ac3H11_1823 0.22 +0.8 26,575 + Ac3H11_1823 0.54 -0.4 26,575 + Ac3H11_1823 0.54 -0.2 26,575 + Ac3H11_1823 0.54 -0.6 26,575 + Ac3H11_1823 0.54 +0.2 26,575 + Ac3H11_1823 0.54 -0.1 26,576 - Ac3H11_1823 0.54 -0.2 26,596 + Ac3H11_1823 0.58 -0.2 26,596 + Ac3H11_1823 0.58 -0.8 26,596 + Ac3H11_1823 0.58 +0.9 26,597 - Ac3H11_1823 0.59 +1.3
Or see this region's nucleotide sequence