Experiment: MoYLS4 with Polymyxin B sulfate 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_2856 and DvMF_2857 are separated by 3 nucleotides DvMF_2857 and DvMF_2858 are separated by 105 nucleotides DvMF_2858 and DvMF_2859 are separated by 178 nucleotides
DvMF_2856: DvMF_2856 - NADPH-dependent FMN reductase (RefSeq), at 3,608,395 to 3,609,027
_2856
DvMF_2857: DvMF_2857 - phosphoribosyltransferase (RefSeq), at 3,609,031 to 3,609,855
_2857
DvMF_2858: DvMF_2858 - short-chain dehydrogenase/reductase SDR (RefSeq), at 3,609,961 to 3,610,698
_2858
DvMF_2859: DvMF_2859 - hypothetical protein (RefSeq), at 3,610,877 to 3,611,665
_2859
Position (kb)
3609
3610
3611 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3609.050 kb on + strand at 3609.336 kb on + strand, within DvMF_2857 at 3609.366 kb on + strand, within DvMF_2857 at 3609.366 kb on + strand, within DvMF_2857 at 3609.371 kb on + strand, within DvMF_2857 at 3609.550 kb on - strand, within DvMF_2857 at 3609.925 kb on + strand at 3610.007 kb on + strand at 3610.136 kb on - strand, within DvMF_2858 at 3610.219 kb on + strand, within DvMF_2858 at 3610.414 kb on - strand, within DvMF_2858 at 3610.469 kb on - strand, within DvMF_2858 at 3610.504 kb on - strand, within DvMF_2858 at 3610.728 kb on - strand at 3610.770 kb on - strand at 3611.437 kb on - strand, within DvMF_2859
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Polymyxin B sulfate 0.25 mg/ml remove 3,609,050 + -1.3 3,609,336 + DvMF_2857 0.37 -0.9 3,609,366 + DvMF_2857 0.41 -0.6 3,609,366 + DvMF_2857 0.41 -0.9 3,609,371 + DvMF_2857 0.41 -1.0 3,609,550 - DvMF_2857 0.63 -1.3 3,609,925 + -0.7 3,610,007 + -0.5 3,610,136 - DvMF_2858 0.24 -0.8 3,610,219 + DvMF_2858 0.35 -1.0 3,610,414 - DvMF_2858 0.61 +2.2 3,610,469 - DvMF_2858 0.69 +0.0 3,610,504 - DvMF_2858 0.74 -0.9 3,610,728 - -1.6 3,610,770 - -1.4 3,611,437 - DvMF_2859 0.71 -2.1
Or see this region's nucleotide sequence