Experiment: MoYLS4 with Polymyxin B sulfate 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_2612 and nifH are separated by 604 nucleotides nifH and DvMF_2614 are separated by 101 nucleotides DvMF_2614 and DvMF_2615 overlap by 4 nucleotides
DvMF_2612: DvMF_2612 - pyruvate carboxyltransferase (RefSeq), at 3,300,432 to 3,301,799
_2612
DvMF_2613: nifH - nitrogenase reductase (RefSeq), at 3,302,404 to 3,303,234
nifH
DvMF_2614: DvMF_2614 - nitrogen regulatory protein P-II (RefSeq), at 3,303,336 to 3,303,686
_2614
DvMF_2615: DvMF_2615 - nitrogen regulatory protein P-II (RefSeq), at 3,303,683 to 3,304,057
_2615
Position (kb)
3302
3303
3304 Strain fitness (log2 ratio)
-2
-1
0
1 at 3301.488 kb on + strand, within DvMF_2612 at 3301.672 kb on + strand at 3301.738 kb on + strand at 3301.781 kb on + strand at 3301.786 kb on + strand at 3302.220 kb on + strand at 3302.317 kb on - strand at 3302.380 kb on + strand at 3302.453 kb on - strand at 3302.455 kb on + strand at 3302.685 kb on - strand, within nifH at 3302.826 kb on + strand, within nifH at 3303.112 kb on + strand, within nifH at 3303.120 kb on - strand, within nifH at 3303.222 kb on + strand at 3303.222 kb on + strand at 3303.237 kb on - strand at 3303.269 kb on + strand at 3303.292 kb on + strand at 3303.637 kb on - strand, within DvMF_2614 at 3303.637 kb on - strand, within DvMF_2614 at 3303.681 kb on + strand at 3303.744 kb on - strand, within DvMF_2615 at 3303.790 kb on + strand, within DvMF_2615 at 3303.810 kb on - strand, within DvMF_2615 at 3303.837 kb on + strand, within DvMF_2615 at 3303.912 kb on - strand, within DvMF_2615 at 3303.951 kb on + strand, within DvMF_2615 at 3303.959 kb on - strand, within DvMF_2615 at 3304.102 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Polymyxin B sulfate 0.25 mg/ml remove 3,301,488 + DvMF_2612 0.77 +0.2 3,301,672 + -0.3 3,301,738 + -2.4 3,301,781 + +0.1 3,301,786 + +0.8 3,302,220 + -1.9 3,302,317 - -0.7 3,302,380 + -0.5 3,302,453 - +0.9 3,302,455 + -1.7 3,302,685 - nifH DvMF_2613 0.34 -1.1 3,302,826 + nifH DvMF_2613 0.51 -0.2 3,303,112 + nifH DvMF_2613 0.85 -0.3 3,303,120 - nifH DvMF_2613 0.86 -0.2 3,303,222 + -1.4 3,303,222 + -1.1 3,303,237 - +0.3 3,303,269 + +0.2 3,303,292 + -1.1 3,303,637 - DvMF_2614 0.86 -0.6 3,303,637 - DvMF_2614 0.86 +0.4 3,303,681 + +0.0 3,303,744 - DvMF_2615 0.16 +0.9 3,303,790 + DvMF_2615 0.29 +1.1 3,303,810 - DvMF_2615 0.34 -0.4 3,303,837 + DvMF_2615 0.41 -0.4 3,303,912 - DvMF_2615 0.61 -0.6 3,303,951 + DvMF_2615 0.71 +0.7 3,303,959 - DvMF_2615 0.74 +0.1 3,304,102 - -1.7
Or see this region's nucleotide sequence