Experiment: MoYLS4 with Polymyxin B sulfate 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_2269 and DvMF_2270 are separated by 18 nucleotides DvMF_2270 and DvMF_2271 are separated by 128 nucleotides DvMF_2271 and DvMF_2272 are separated by 325 nucleotides
DvMF_2269: DvMF_2269 - DNA polymerase III, subunits gamma and tau (RefSeq), at 2,851,219 to 2,853,285
_2269
DvMF_2270: DvMF_2270 - hypothetical protein (RefSeq), at 2,853,304 to 2,853,615
_2270
DvMF_2271: DvMF_2271 - recombination protein RecR (RefSeq), at 2,853,744 to 2,854,349
_2271
DvMF_2272: DvMF_2272 - hypothetical protein (RefSeq), at 2,854,675 to 2,855,430
_2272
Position (kb)
2853
2854
2855 Strain fitness (log2 ratio)
-2
-1
0
1 at 2853.726 kb on + strand at 2853.885 kb on + strand, within DvMF_2271 at 2853.947 kb on + strand, within DvMF_2271 at 2854.032 kb on - strand, within DvMF_2271 at 2854.050 kb on + strand, within DvMF_2271 at 2854.164 kb on + strand, within DvMF_2271 at 2854.172 kb on - strand, within DvMF_2271 at 2854.177 kb on + strand, within DvMF_2271 at 2854.262 kb on + strand, within DvMF_2271 at 2854.336 kb on + strand at 2854.422 kb on - strand at 2854.451 kb on + strand at 2854.649 kb on - strand at 2854.681 kb on - strand at 2854.747 kb on + strand at 2854.762 kb on - strand, within DvMF_2272 at 2854.873 kb on - strand, within DvMF_2272 at 2855.006 kb on + strand, within DvMF_2272
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Polymyxin B sulfate 0.25 mg/ml remove 2,853,726 + -2.2 2,853,885 + DvMF_2271 0.23 -0.4 2,853,947 + DvMF_2271 0.33 -0.3 2,854,032 - DvMF_2271 0.48 -1.7 2,854,050 + DvMF_2271 0.50 -1.5 2,854,164 + DvMF_2271 0.69 -1.3 2,854,172 - DvMF_2271 0.71 -1.5 2,854,177 + DvMF_2271 0.71 -1.4 2,854,262 + DvMF_2271 0.85 -0.1 2,854,336 + -1.0 2,854,422 - -0.3 2,854,451 + -0.5 2,854,649 - -0.7 2,854,681 - -0.3 2,854,747 + -1.0 2,854,762 - DvMF_2272 0.12 -1.0 2,854,873 - DvMF_2272 0.26 -0.7 2,855,006 + DvMF_2272 0.44 +0.2
Or see this region's nucleotide sequence