Experiment: MoYLS4 with Polymyxin B sulfate 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_R0046 and DvMF_R0047 are separated by 6 nucleotides DvMF_R0047 and DvMF_1578 are separated by 560 nucleotides DvMF_1578 and DvMF_1579 are separated by 37 nucleotides DvMF_1579 and DvMF_1580 are separated by 758 nucleotides
DvMF_R0046: DvMF_R0046 - tRNA-Asp (RefSeq), at 1,946,388 to 1,946,464
_R0046
DvMF_R0047: DvMF_R0047 - tRNA-Val (RefSeq), at 1,946,471 to 1,946,546
_R0047
DvMF_1578: DvMF_1578 - hypothetical protein (RefSeq), at 1,947,107 to 1,947,307
_1578
DvMF_1579: DvMF_1579 - polysaccharide deacetylase (RefSeq), at 1,947,345 to 1,948,133
_1579
DvMF_1580: DvMF_1580 - Extracellular ligand-binding receptor (RefSeq), at 1,948,892 to 1,950,076
_1580
Position (kb) 1947
1948
1949 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 1946.713 kb on + strand at 1947.527 kb on + strand, within DvMF_1579 at 1947.717 kb on - strand, within DvMF_1579 at 1947.888 kb on - strand, within DvMF_1579 at 1947.994 kb on + strand, within DvMF_1579 at 1948.173 kb on - strand at 1948.184 kb on + strand at 1948.353 kb on - strand at 1948.372 kb on - strand at 1948.934 kb on + strand at 1949.002 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Polymyxin B sulfate 0.25 mg/ml remove 1,946,713 + -0.3 1,947,527 + DvMF_1579 0.23 -0.4 1,947,717 - DvMF_1579 0.47 -1.3 1,947,888 - DvMF_1579 0.69 -3.0 1,947,994 + DvMF_1579 0.82 -1.3 1,948,173 - -0.4 1,948,184 + -1.5 1,948,353 - -0.5 1,948,372 - -0.3 1,948,934 + -0.6 1,949,002 + -0.2
Or see this region's nucleotide sequence