Experiment: MoYLS4 with Polymyxin B sulfate 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_1327 and DvMF_1328 are separated by 145 nucleotides DvMF_1328 and DvMF_1329 are separated by 290 nucleotides DvMF_1329 and DvMF_1330 are separated by 75 nucleotides
DvMF_1327: DvMF_1327 - hypothetical protein (RefSeq), at 1,622,511 to 1,623,554
_1327
DvMF_1328: DvMF_1328 - heavy metal-binding domain-containing protein (RefSeq), at 1,623,700 to 1,623,909
_1328
DvMF_1329: DvMF_1329 - hypothetical protein (RefSeq), at 1,624,200 to 1,624,493
_1329
DvMF_1330: DvMF_1330 - RNA modification enzyme, MiaB family (RefSeq), at 1,624,569 to 1,626,005
_1330
Position (kb)
1623
1624 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1623.040 kb on + strand, within DvMF_1327 at 1623.291 kb on + strand, within DvMF_1327 at 1623.326 kb on - strand, within DvMF_1327 at 1623.477 kb on - strand at 1623.744 kb on - strand, within DvMF_1328 at 1623.887 kb on + strand, within DvMF_1328 at 1624.094 kb on - strand at 1624.126 kb on + strand at 1624.250 kb on - strand, within DvMF_1329 at 1624.349 kb on + strand, within DvMF_1329 at 1624.414 kb on - strand, within DvMF_1329 at 1624.450 kb on - strand, within DvMF_1329 at 1624.512 kb on + strand at 1624.666 kb on - strand at 1624.716 kb on + strand, within DvMF_1330 at 1624.757 kb on - strand, within DvMF_1330 at 1624.821 kb on - strand, within DvMF_1330
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Polymyxin B sulfate 0.25 mg/ml remove 1,623,040 + DvMF_1327 0.51 -0.9 1,623,291 + DvMF_1327 0.75 -1.0 1,623,326 - DvMF_1327 0.78 +0.5 1,623,477 - -1.0 1,623,744 - DvMF_1328 0.21 -0.8 1,623,887 + DvMF_1328 0.89 -3.3 1,624,094 - -0.5 1,624,126 + -0.5 1,624,250 - DvMF_1329 0.17 +1.5 1,624,349 + DvMF_1329 0.51 -0.1 1,624,414 - DvMF_1329 0.73 +0.5 1,624,450 - DvMF_1329 0.85 -0.4 1,624,512 + -0.2 1,624,666 - -1.4 1,624,716 + DvMF_1330 0.10 -0.7 1,624,757 - DvMF_1330 0.13 +0.1 1,624,821 - DvMF_1330 0.18 -1.0
Or see this region's nucleotide sequence