Experiment: MoYLS4 with Polymyxin B sulfate 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0643 and DvMF_0644 are separated by 10 nucleotides DvMF_0644 and DvMF_0645 are separated by 292 nucleotides DvMF_0645 and DvMF_0646 are separated by 744 nucleotides
DvMF_0643: DvMF_0643 - formate dehydrogenase, alpha subunit (RefSeq), at 770,931 to 773,969
_0643
DvMF_0644: DvMF_0644 - formate dehydrogenase, beta subunit, putative (RefSeq), at 773,980 to 774,627
_0644
DvMF_0645: DvMF_0645 - formate dehydrogenase accessory protein (RefSeq), at 774,920 to 775,933
_0645
DvMF_0646: DvMF_0646 - formate dehydrogenase, alpha subunit (RefSeq), at 776,678 to 779,719
_0646
Position (kb) 774
775
776 Strain fitness (log2 ratio) -2
-1
0
1 at 774.003 kb on + strand at 774.026 kb on + strand at 774.132 kb on + strand, within DvMF_0644 at 774.164 kb on - strand, within DvMF_0644 at 774.285 kb on + strand, within DvMF_0644 at 774.293 kb on - strand, within DvMF_0644 at 774.742 kb on + strand at 774.974 kb on + strand at 775.193 kb on - strand, within DvMF_0645 at 775.193 kb on - strand, within DvMF_0645 at 775.231 kb on - strand, within DvMF_0645 at 775.680 kb on + strand, within DvMF_0645 at 775.680 kb on + strand, within DvMF_0645 at 775.868 kb on - strand at 776.504 kb on + strand at 776.656 kb on + strand at 776.707 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Polymyxin B sulfate 0.25 mg/ml remove 774,003 + -1.1 774,026 + -0.2 774,132 + DvMF_0644 0.23 -0.7 774,164 - DvMF_0644 0.28 -0.4 774,285 + DvMF_0644 0.47 -2.6 774,293 - DvMF_0644 0.48 -0.1 774,742 + -1.0 774,974 + -0.2 775,193 - DvMF_0645 0.27 +0.7 775,193 - DvMF_0645 0.27 -0.6 775,231 - DvMF_0645 0.31 -0.6 775,680 + DvMF_0645 0.75 -0.7 775,680 + DvMF_0645 0.75 +0.2 775,868 - -0.9 776,504 + +0.6 776,656 + +0.2 776,707 + -0.8
Or see this region's nucleotide sequence