Experiment: MoYLS4 with Polymyxin B sulfate 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0600 and DvMF_0601 are separated by 386 nucleotides DvMF_0601 and DvMF_0602 are separated by 517 nucleotides DvMF_0602 and DvMF_0603 are separated by 4 nucleotides
DvMF_0600: DvMF_0600 - degV family protein (RefSeq), at 712,037 to 714,088
_0600
DvMF_0601: DvMF_0601 - hypothetical protein (RefSeq), at 714,475 to 714,708
_0601
DvMF_0602: DvMF_0602 - 4Fe-4S ferredoxin iron-sulfur binding domain protein (RefSeq), at 715,226 to 715,552
_0602
DvMF_0603: DvMF_0603 - 2-oxoglutarate ferredoxin oxidoreductase subunit alpha (RefSeq), at 715,557 to 716,705
_0603
Position (kb) 714
715 Strain fitness (log2 ratio) -2
-1
0
1 at 713.495 kb on + strand, within DvMF_0600 at 713.503 kb on - strand, within DvMF_0600 at 713.577 kb on + strand, within DvMF_0600 at 713.597 kb on - strand, within DvMF_0600 at 713.801 kb on - strand, within DvMF_0600 at 713.811 kb on - strand, within DvMF_0600 at 713.867 kb on - strand, within DvMF_0600 at 713.990 kb on + strand at 714.159 kb on + strand at 714.594 kb on + strand, within DvMF_0601 at 714.602 kb on - strand, within DvMF_0601 at 714.888 kb on + strand at 715.034 kb on + strand at 715.309 kb on - strand, within DvMF_0602 at 715.401 kb on + strand, within DvMF_0602 at 715.404 kb on - strand, within DvMF_0602 at 715.541 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Polymyxin B sulfate 0.25 mg/ml remove 713,495 + DvMF_0600 0.71 -0.1 713,503 - DvMF_0600 0.71 -0.4 713,577 + DvMF_0600 0.75 -0.1 713,597 - DvMF_0600 0.76 -1.5 713,801 - DvMF_0600 0.86 -0.7 713,811 - DvMF_0600 0.86 +0.5 713,867 - DvMF_0600 0.89 +0.6 713,990 + -0.9 714,159 + +1.1 714,594 + DvMF_0601 0.51 -1.4 714,602 - DvMF_0601 0.54 -1.3 714,888 + -0.6 715,034 + -0.1 715,309 - DvMF_0602 0.25 -0.6 715,401 + DvMF_0602 0.54 -2.6 715,404 - DvMF_0602 0.54 -0.5 715,541 + -0.9
Or see this region's nucleotide sequence