Experiment: MoYLS4 with Polymyxin B sulfate 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0550 and DvMF_0551 are separated by 67 nucleotides DvMF_0551 and DvMF_0552 are separated by 347 nucleotides
DvMF_0550: DvMF_0550 - diaminopimelate aminotransferase (RefSeq), at 647,739 to 648,968
_0550
DvMF_0551: DvMF_0551 - tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA (RefSeq), at 649,036 to 650,937
_0551
DvMF_0552: DvMF_0552 - hypothetical protein (RefSeq), at 651,285 to 652,688
_0552
Position (kb) 649
650
651 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 648.067 kb on - strand, within DvMF_0550 at 648.111 kb on - strand, within DvMF_0550 at 648.183 kb on - strand, within DvMF_0550 at 648.241 kb on - strand, within DvMF_0550 at 648.361 kb on + strand, within DvMF_0550 at 648.383 kb on + strand, within DvMF_0550 at 648.391 kb on - strand, within DvMF_0550 at 648.444 kb on - strand, within DvMF_0550 at 648.480 kb on - strand, within DvMF_0550 at 648.556 kb on - strand, within DvMF_0550 at 648.556 kb on - strand, within DvMF_0550 at 648.556 kb on - strand, within DvMF_0550 at 648.806 kb on - strand, within DvMF_0550 at 648.993 kb on - strand at 649.031 kb on + strand at 649.085 kb on + strand at 649.134 kb on - strand at 649.303 kb on - strand, within DvMF_0551 at 649.446 kb on - strand, within DvMF_0551 at 649.781 kb on + strand, within DvMF_0551 at 649.816 kb on + strand, within DvMF_0551 at 650.000 kb on - strand, within DvMF_0551 at 650.216 kb on + strand, within DvMF_0551 at 650.480 kb on + strand, within DvMF_0551 at 650.632 kb on + strand, within DvMF_0551 at 650.831 kb on + strand at 650.839 kb on - strand at 650.852 kb on + strand at 650.872 kb on + strand at 651.015 kb on + strand at 651.151 kb on - strand at 651.154 kb on + strand at 651.168 kb on + strand at 651.234 kb on - strand at 651.262 kb on + strand at 651.262 kb on + strand at 651.262 kb on + strand at 651.282 kb on + strand at 651.290 kb on - strand at 651.349 kb on - strand at 651.369 kb on - strand at 651.681 kb on - strand, within DvMF_0552 at 651.744 kb on - strand, within DvMF_0552 at 651.761 kb on - strand, within DvMF_0552 at 651.797 kb on + strand, within DvMF_0552 at 651.841 kb on - strand, within DvMF_0552 at 651.858 kb on - strand, within DvMF_0552
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Polymyxin B sulfate 0.25 mg/ml remove 648,067 - DvMF_0550 0.27 +0.1 648,111 - DvMF_0550 0.30 -0.6 648,183 - DvMF_0550 0.36 +0.1 648,241 - DvMF_0550 0.41 -1.2 648,361 + DvMF_0550 0.51 -4.6 648,383 + DvMF_0550 0.52 -0.9 648,391 - DvMF_0550 0.53 -1.1 648,444 - DvMF_0550 0.57 -0.7 648,480 - DvMF_0550 0.60 -0.7 648,556 - DvMF_0550 0.66 -0.1 648,556 - DvMF_0550 0.66 -0.9 648,556 - DvMF_0550 0.66 -0.2 648,806 - DvMF_0550 0.87 +1.0 648,993 - -0.9 649,031 + -0.7 649,085 + -2.4 649,134 - +0.3 649,303 - DvMF_0551 0.14 +2.0 649,446 - DvMF_0551 0.22 +1.1 649,781 + DvMF_0551 0.39 -0.6 649,816 + DvMF_0551 0.41 +1.4 650,000 - DvMF_0551 0.51 -0.4 650,216 + DvMF_0551 0.62 -0.7 650,480 + DvMF_0551 0.76 +0.2 650,632 + DvMF_0551 0.84 -0.5 650,831 + -0.6 650,839 - -0.2 650,852 + +0.1 650,872 + +2.1 651,015 + -0.4 651,151 - -4.2 651,154 + +0.6 651,168 + -3.5 651,234 - -3.7 651,262 + -0.5 651,262 + -1.1 651,262 + -0.0 651,282 + -2.2 651,290 - -0.2 651,349 - +0.3 651,369 - -1.0 651,681 - DvMF_0552 0.28 -0.5 651,744 - DvMF_0552 0.33 -3.1 651,761 - DvMF_0552 0.34 -0.7 651,797 + DvMF_0552 0.36 -2.9 651,841 - DvMF_0552 0.40 -0.8 651,858 - DvMF_0552 0.41 -1.9
Or see this region's nucleotide sequence