Experiment: MoYLS4 with Polymyxin B sulfate 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0195 and DvMF_0196 are separated by 97 nucleotides DvMF_0196 and DvMF_0197 are separated by 330 nucleotides DvMF_0197 and DvMF_0198 are separated by 383 nucleotides DvMF_0198 and DvMF_0199 are separated by 114 nucleotides
DvMF_0195: DvMF_0195 - RNP-1 like RNA-binding protein (RefSeq), at 214,229 to 214,495
_0195
DvMF_0196: DvMF_0196 - translation initiation factor IF-1 (RefSeq), at 214,593 to 214,811
_0196
DvMF_0197: DvMF_0197 - 4Fe-4S ferredoxin iron-sulfur binding domain protein (RefSeq), at 215,142 to 216,617
_0197
DvMF_0198: DvMF_0198 - hypothetical protein (RefSeq), at 217,001 to 217,324
_0198
DvMF_0199: DvMF_0199 - ATP:corrinoid adenosyltransferase BtuR/CobO/CobP (RefSeq), at 217,439 to 217,942
_0199
Position (kb) 215
216
217 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 214.443 kb on + strand, within DvMF_0195 at 214.451 kb on - strand, within DvMF_0195 at 214.897 kb on + strand at 214.939 kb on - strand at 215.091 kb on - strand at 215.233 kb on + strand at 215.428 kb on - strand, within DvMF_0197 at 215.808 kb on + strand, within DvMF_0197 at 216.061 kb on + strand, within DvMF_0197 at 216.069 kb on - strand, within DvMF_0197 at 216.069 kb on - strand, within DvMF_0197 at 216.092 kb on - strand, within DvMF_0197 at 216.176 kb on + strand, within DvMF_0197 at 216.218 kb on - strand, within DvMF_0197 at 216.321 kb on + strand, within DvMF_0197 at 216.348 kb on + strand, within DvMF_0197 at 216.348 kb on + strand, within DvMF_0197 at 216.399 kb on + strand, within DvMF_0197 at 216.418 kb on + strand, within DvMF_0197 at 216.587 kb on + strand at 216.668 kb on - strand at 216.967 kb on - strand at 217.271 kb on + strand, within DvMF_0198 at 217.271 kb on + strand, within DvMF_0198 at 217.271 kb on + strand, within DvMF_0198 at 217.271 kb on + strand, within DvMF_0198 at 217.271 kb on + strand, within DvMF_0198 at 217.279 kb on - strand, within DvMF_0198 at 217.279 kb on - strand, within DvMF_0198 at 217.429 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Polymyxin B sulfate 0.25 mg/ml remove 214,443 + DvMF_0195 0.80 -0.6 214,451 - DvMF_0195 0.83 -1.5 214,897 + +2.4 214,939 - -1.1 215,091 - -0.3 215,233 + +0.7 215,428 - DvMF_0197 0.19 +0.2 215,808 + DvMF_0197 0.45 +0.4 216,061 + DvMF_0197 0.62 -1.1 216,069 - DvMF_0197 0.63 -0.3 216,069 - DvMF_0197 0.63 +3.2 216,092 - DvMF_0197 0.64 +0.4 216,176 + DvMF_0197 0.70 -0.4 216,218 - DvMF_0197 0.73 -1.5 216,321 + DvMF_0197 0.80 -0.5 216,348 + DvMF_0197 0.82 +0.2 216,348 + DvMF_0197 0.82 -0.6 216,399 + DvMF_0197 0.85 -0.5 216,418 + DvMF_0197 0.86 -0.9 216,587 + -1.0 216,668 - +1.9 216,967 - -0.5 217,271 + DvMF_0198 0.83 -0.5 217,271 + DvMF_0198 0.83 -0.9 217,271 + DvMF_0198 0.83 -0.6 217,271 + DvMF_0198 0.83 -3.3 217,271 + DvMF_0198 0.83 +0.5 217,279 - DvMF_0198 0.86 -0.3 217,279 - DvMF_0198 0.86 -3.7 217,429 + -0.2
Or see this region's nucleotide sequence