Experiment: MoYLS4 with Polymyxin B sulfate 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0128 and DvMF_0129 are separated by 192 nucleotides DvMF_0129 and DvMF_0130 are separated by 58 nucleotides DvMF_0130 and DvMF_0131 are separated by 360 nucleotides DvMF_0131 and DvMF_0132 are separated by 2 nucleotides
DvMF_0128: DvMF_0128 - multiple antibiotic resistance (MarC)-related protein (RefSeq), at 142,355 to 142,972
_0128
DvMF_0129: DvMF_0129 - 6-pyruvoyl tetrahydropterin synthase and hypothetical protein (RefSeq), at 143,165 to 143,557
_0129
DvMF_0130: DvMF_0130 - DNA polymerase III, alpha subunit (RefSeq), at 143,616 to 147,137
_0130
DvMF_0131: DvMF_0131 - hypothetical protein (RefSeq), at 147,498 to 147,839
_0131
DvMF_0132: DvMF_0132 - metal-dependent phosphohydrolase HD region (RefSeq), at 147,842 to 148,711
_0132
Position (kb) 143
144
145
146
147
148 Strain fitness (log2 ratio) -2
-1
0
1 at 142.741 kb on + strand, within DvMF_0128 at 142.847 kb on + strand, within DvMF_0128 at 147.377 kb on + strand at 147.447 kb on + strand at 147.675 kb on + strand, within DvMF_0131 at 147.795 kb on - strand, within DvMF_0131 at 147.882 kb on - strand at 148.010 kb on + strand, within DvMF_0132 at 148.019 kb on + strand, within DvMF_0132 at 148.048 kb on - strand, within DvMF_0132 at 148.073 kb on - strand, within DvMF_0132
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Polymyxin B sulfate 0.25 mg/ml remove 142,741 + DvMF_0128 0.62 -0.4 142,847 + DvMF_0128 0.80 -1.4 147,377 + -0.4 147,447 + -0.0 147,675 + DvMF_0131 0.52 -2.2 147,795 - DvMF_0131 0.87 -1.4 147,882 - +0.1 148,010 + DvMF_0132 0.19 -1.5 148,019 + DvMF_0132 0.20 +0.4 148,048 - DvMF_0132 0.24 -0.6 148,073 - DvMF_0132 0.27 +0.7
Or see this region's nucleotide sequence