Experiment: pyruvate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0289 and ilvM overlap by 4 nucleotides ilvM and Shew_0291 are separated by 26 nucleotides Shew_0291 and Shew_0292 are separated by 112 nucleotides
Shew_0289: Shew_0289 - acetolactate synthase 2 catalytic subunit (RefSeq), at 308,543 to 310,216
_0289
Shew_0290: ilvM - acetolactate synthase 2 regulatory subunit (RefSeq), at 310,213 to 310,482
ilvM
Shew_0291: Shew_0291 - branched-chain amino acid aminotransferase (RefSeq), at 310,509 to 311,432
_0291
Shew_0292: Shew_0292 - dihydroxy-acid dehydratase (RefSeq), at 311,545 to 313,395
_0292
Position (kb) 310
311
312 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 309.543 kb on + strand, within Shew_0289 at 309.697 kb on + strand, within Shew_0289 at 309.730 kb on + strand, within Shew_0289 at 309.763 kb on - strand, within Shew_0289 at 309.820 kb on - strand, within Shew_0289 at 310.036 kb on - strand, within Shew_0289 at 310.141 kb on + strand at 310.300 kb on - strand, within ilvM at 310.347 kb on + strand, within ilvM at 310.655 kb on + strand, within Shew_0291 at 311.098 kb on + strand, within Shew_0291 at 311.106 kb on - strand, within Shew_0291 at 311.129 kb on + strand, within Shew_0291 at 311.137 kb on - strand, within Shew_0291 at 311.137 kb on - strand, within Shew_0291 at 311.137 kb on - strand, within Shew_0291 at 311.137 kb on - strand, within Shew_0291 at 311.424 kb on - strand at 311.424 kb on - strand at 311.459 kb on + strand at 311.669 kb on + strand at 311.763 kb on + strand, within Shew_0292 at 311.814 kb on + strand, within Shew_0292 at 311.814 kb on + strand, within Shew_0292 at 311.831 kb on + strand, within Shew_0292 at 311.934 kb on - strand, within Shew_0292 at 312.076 kb on + strand, within Shew_0292 at 312.166 kb on + strand, within Shew_0292 at 312.326 kb on + strand, within Shew_0292 at 312.334 kb on - strand, within Shew_0292
Per-strain Table
Position Strand Gene LocusTag Fraction pyruvate (C) remove 309,543 + Shew_0289 0.60 -4.5 309,697 + Shew_0289 0.69 -3.4 309,730 + Shew_0289 0.71 -2.4 309,763 - Shew_0289 0.73 -4.4 309,820 - Shew_0289 0.76 -3.2 310,036 - Shew_0289 0.89 -4.3 310,141 + -3.7 310,300 - ilvM Shew_0290 0.32 -4.2 310,347 + ilvM Shew_0290 0.50 -2.5 310,655 + Shew_0291 0.16 -2.4 311,098 + Shew_0291 0.64 -2.3 311,106 - Shew_0291 0.65 -4.7 311,129 + Shew_0291 0.67 -2.3 311,137 - Shew_0291 0.68 -4.9 311,137 - Shew_0291 0.68 -3.5 311,137 - Shew_0291 0.68 -3.6 311,137 - Shew_0291 0.68 -4.0 311,424 - -3.0 311,424 - -3.4 311,459 + -2.8 311,669 + -4.5 311,763 + Shew_0292 0.12 -3.7 311,814 + Shew_0292 0.15 -4.8 311,814 + Shew_0292 0.15 -2.1 311,831 + Shew_0292 0.15 -4.7 311,934 - Shew_0292 0.21 -4.6 312,076 + Shew_0292 0.29 -1.4 312,166 + Shew_0292 0.34 -2.8 312,326 + Shew_0292 0.42 -4.3 312,334 - Shew_0292 0.43 -3.2
Or see this region's nucleotide sequence