Experiment: R2A with Nickel (II) chloride 0.3125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK54_RS07030 and LRK54_RS07035 are separated by 67 nucleotides LRK54_RS07035 and LRK54_RS07040 are separated by 187 nucleotides LRK54_RS07040 and LRK54_RS07045 overlap by 23 nucleotides
LRK54_RS07030: LRK54_RS07030 - tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD, at 1,507,635 to 1,508,684
_RS07030
LRK54_RS07035: LRK54_RS07035 - 30S ribosomal protein S21, at 1,508,752 to 1,508,967
_RS07035
LRK54_RS07040: LRK54_RS07040 - GatB/YqeY domain-containing protein, at 1,509,155 to 1,509,601
_RS07040
LRK54_RS07045: LRK54_RS07045 - hypothetical protein, at 1,509,579 to 1,509,893
_RS07045
Position (kb)
1508
1509 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1508.962 kb on + strand at 1508.962 kb on + strand at 1508.962 kb on + strand at 1508.962 kb on + strand at 1508.962 kb on + strand at 1508.963 kb on - strand at 1508.963 kb on - strand at 1508.963 kb on - strand at 1508.963 kb on - strand at 1508.963 kb on - strand at 1508.963 kb on - strand at 1508.963 kb on - strand at 1508.963 kb on - strand at 1508.963 kb on - strand at 1509.120 kb on + strand at 1509.122 kb on + strand at 1509.122 kb on + strand at 1509.122 kb on + strand at 1509.123 kb on - strand at 1509.123 kb on - strand at 1509.123 kb on - strand at 1509.123 kb on - strand at 1509.354 kb on - strand, within LRK54_RS07040 at 1509.354 kb on - strand, within LRK54_RS07040 at 1509.419 kb on + strand, within LRK54_RS07040 at 1509.419 kb on + strand, within LRK54_RS07040 at 1509.419 kb on + strand, within LRK54_RS07040 at 1509.419 kb on + strand, within LRK54_RS07040 at 1509.419 kb on + strand, within LRK54_RS07040 at 1509.419 kb on + strand, within LRK54_RS07040 at 1509.419 kb on + strand, within LRK54_RS07040 at 1509.419 kb on + strand, within LRK54_RS07040 at 1509.419 kb on + strand, within LRK54_RS07040 at 1509.419 kb on + strand, within LRK54_RS07040 at 1509.419 kb on + strand, within LRK54_RS07040 at 1509.419 kb on + strand, within LRK54_RS07040 at 1509.419 kb on + strand, within LRK54_RS07040 at 1509.420 kb on - strand, within LRK54_RS07040 at 1509.420 kb on - strand, within LRK54_RS07040 at 1509.420 kb on - strand, within LRK54_RS07040 at 1509.420 kb on - strand, within LRK54_RS07040 at 1509.420 kb on - strand, within LRK54_RS07040 at 1509.420 kb on - strand, within LRK54_RS07040 at 1509.420 kb on - strand, within LRK54_RS07040 at 1509.420 kb on - strand, within LRK54_RS07040 at 1509.420 kb on - strand, within LRK54_RS07040 at 1509.715 kb on + strand, within LRK54_RS07045 at 1509.715 kb on + strand, within LRK54_RS07045 at 1509.715 kb on + strand, within LRK54_RS07045 at 1509.715 kb on + strand, within LRK54_RS07045 at 1509.715 kb on + strand, within LRK54_RS07045 at 1509.716 kb on - strand, within LRK54_RS07045 at 1509.716 kb on - strand, within LRK54_RS07045 at 1509.716 kb on - strand, within LRK54_RS07045 at 1509.716 kb on - strand, within LRK54_RS07045 at 1509.716 kb on - strand, within LRK54_RS07045 at 1509.716 kb on - strand, within LRK54_RS07045 at 1509.716 kb on - strand, within LRK54_RS07045 at 1509.716 kb on - strand, within LRK54_RS07045 at 1509.716 kb on - strand, within LRK54_RS07045 at 1509.822 kb on - strand, within LRK54_RS07045 at 1509.822 kb on - strand, within LRK54_RS07045 at 1509.881 kb on + strand at 1509.881 kb on + strand at 1509.881 kb on + strand at 1509.881 kb on + strand at 1509.881 kb on + strand at 1509.881 kb on + strand at 1509.881 kb on + strand at 1509.881 kb on + strand at 1509.881 kb on + strand at 1509.882 kb on - strand at 1509.882 kb on - strand at 1509.882 kb on - strand at 1509.882 kb on - strand at 1509.882 kb on - strand at 1509.882 kb on - strand at 1509.882 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.3125 mM remove 1,508,962 + +0.3 1,508,962 + -0.9 1,508,962 + -0.1 1,508,962 + -0.6 1,508,962 + +4.3 1,508,963 - -0.2 1,508,963 - +0.3 1,508,963 - -1.4 1,508,963 - +0.5 1,508,963 - +0.1 1,508,963 - -0.1 1,508,963 - +0.2 1,508,963 - +0.1 1,508,963 - -1.3 1,509,120 + +0.2 1,509,122 + +0.2 1,509,122 + +0.4 1,509,122 + +0.7 1,509,123 - +1.0 1,509,123 - +0.0 1,509,123 - +1.5 1,509,123 - -0.1 1,509,354 - LRK54_RS07040 0.45 -1.7 1,509,354 - LRK54_RS07040 0.45 -0.8 1,509,419 + LRK54_RS07040 0.59 +0.2 1,509,419 + LRK54_RS07040 0.59 -0.6 1,509,419 + LRK54_RS07040 0.59 -0.0 1,509,419 + LRK54_RS07040 0.59 +1.0 1,509,419 + LRK54_RS07040 0.59 +0.4 1,509,419 + LRK54_RS07040 0.59 +0.5 1,509,419 + LRK54_RS07040 0.59 +0.3 1,509,419 + LRK54_RS07040 0.59 +0.0 1,509,419 + LRK54_RS07040 0.59 +1.7 1,509,419 + LRK54_RS07040 0.59 -0.1 1,509,419 + LRK54_RS07040 0.59 +0.0 1,509,419 + LRK54_RS07040 0.59 -1.5 1,509,419 + LRK54_RS07040 0.59 +1.1 1,509,420 - LRK54_RS07040 0.59 +0.0 1,509,420 - LRK54_RS07040 0.59 -0.3 1,509,420 - LRK54_RS07040 0.59 +0.2 1,509,420 - LRK54_RS07040 0.59 +2.3 1,509,420 - LRK54_RS07040 0.59 -0.6 1,509,420 - LRK54_RS07040 0.59 +0.5 1,509,420 - LRK54_RS07040 0.59 -0.4 1,509,420 - LRK54_RS07040 0.59 -0.4 1,509,420 - LRK54_RS07040 0.59 -0.7 1,509,715 + LRK54_RS07045 0.43 +0.7 1,509,715 + LRK54_RS07045 0.43 -0.3 1,509,715 + LRK54_RS07045 0.43 +0.0 1,509,715 + LRK54_RS07045 0.43 -0.3 1,509,715 + LRK54_RS07045 0.43 +0.9 1,509,716 - LRK54_RS07045 0.43 -0.6 1,509,716 - LRK54_RS07045 0.43 -0.3 1,509,716 - LRK54_RS07045 0.43 +2.7 1,509,716 - LRK54_RS07045 0.43 +0.6 1,509,716 - LRK54_RS07045 0.43 -0.7 1,509,716 - LRK54_RS07045 0.43 +0.6 1,509,716 - LRK54_RS07045 0.43 -1.0 1,509,716 - LRK54_RS07045 0.43 -1.4 1,509,716 - LRK54_RS07045 0.43 +1.7 1,509,822 - LRK54_RS07045 0.77 -1.7 1,509,822 - LRK54_RS07045 0.77 -0.0 1,509,881 + -0.9 1,509,881 + +0.1 1,509,881 + -0.5 1,509,881 + -0.4 1,509,881 + -0.5 1,509,881 + -0.7 1,509,881 + -1.9 1,509,881 + -1.3 1,509,881 + +0.7 1,509,882 - -0.1 1,509,882 - +1.0 1,509,882 - -0.8 1,509,882 - +0.0 1,509,882 - -0.3 1,509,882 - +1.7 1,509,882 - +0.1
Or see this region's nucleotide sequence