Experiment: L-Lysine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS10130 and AZOBR_RS10135 are separated by 60 nucleotides AZOBR_RS10135 and AZOBR_RS10140 are separated by 5 nucleotides AZOBR_RS10140 and AZOBR_RS10145 are separated by 209 nucleotides AZOBR_RS10145 and AZOBR_RS10150 overlap by 4 nucleotides
AZOBR_RS10130: AZOBR_RS10130 - required for sulfate utilization, putative electron source for sulfite reductase CysI (from data) , at 2,118,546 to 2,118,845
_RS10130
AZOBR_RS10135: AZOBR_RS10135 - uroporphyrin-III methyltransferase, at 2,118,906 to 2,119,721
_RS10135
AZOBR_RS10140: AZOBR_RS10140 - siroheme synthase, at 2,119,727 to 2,120,284
_RS10140
AZOBR_RS10145: AZOBR_RS10145 - phosphoadenosine phosphosulfate reductase, at 2,120,494 to 2,121,228
_RS10145
AZOBR_RS10150: AZOBR_RS10150 - sulfate adenylyltransferase, at 2,121,225 to 2,122,022
_RS10150
Position (kb)
2119
2120
2121 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 2118.867 kb on - strand at 2118.867 kb on - strand at 2118.867 kb on - strand at 2119.024 kb on - strand, within AZOBR_RS10135 at 2119.024 kb on - strand, within AZOBR_RS10135 at 2119.180 kb on + strand, within AZOBR_RS10135 at 2119.180 kb on + strand, within AZOBR_RS10135 at 2119.181 kb on - strand, within AZOBR_RS10135 at 2119.181 kb on - strand, within AZOBR_RS10135 at 2119.181 kb on - strand, within AZOBR_RS10135 at 2119.181 kb on - strand, within AZOBR_RS10135 at 2119.721 kb on + strand at 2119.722 kb on - strand at 2120.169 kb on + strand, within AZOBR_RS10140 at 2120.170 kb on - strand, within AZOBR_RS10140 at 2120.554 kb on + strand at 2120.555 kb on - strand at 2121.244 kb on - strand at 2121.264 kb on + strand at 2121.264 kb on + strand at 2121.264 kb on + strand at 2121.264 kb on + strand at 2121.264 kb on + strand at 2121.264 kb on + strand at 2121.264 kb on + strand at 2121.264 kb on + strand at 2121.265 kb on - strand at 2121.265 kb on - strand at 2121.265 kb on - strand at 2121.282 kb on + strand at 2121.283 kb on - strand at 2121.283 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Lysine (N) remove 2,118,867 - -2.5 2,118,867 - -3.8 2,118,867 - -3.6 2,119,024 - AZOBR_RS10135 0.14 +0.4 2,119,024 - AZOBR_RS10135 0.14 -1.0 2,119,180 + AZOBR_RS10135 0.34 -5.2 2,119,180 + AZOBR_RS10135 0.34 -0.6 2,119,181 - AZOBR_RS10135 0.34 -1.1 2,119,181 - AZOBR_RS10135 0.34 -0.7 2,119,181 - AZOBR_RS10135 0.34 -0.9 2,119,181 - AZOBR_RS10135 0.34 +0.1 2,119,721 + -4.8 2,119,722 - -3.2 2,120,169 + AZOBR_RS10140 0.79 -4.8 2,120,170 - AZOBR_RS10140 0.79 -4.1 2,120,554 + -0.2 2,120,555 - -2.5 2,121,244 - -0.7 2,121,264 + -2.0 2,121,264 + +0.7 2,121,264 + +2.3 2,121,264 + -3.2 2,121,264 + -1.4 2,121,264 + -2.4 2,121,264 + -1.9 2,121,264 + -3.6 2,121,265 - -4.5 2,121,265 - -2.3 2,121,265 - -0.9 2,121,282 + -3.4 2,121,283 - -3.3 2,121,283 - -3.8
Or see this region's nucleotide sequence