Experiment: D,L-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2822 and Shew_2823 are separated by 195 nucleotides Shew_2823 and rpsO are separated by 107 nucleotides rpsO and Shew_2825 are separated by 95 nucleotides
Shew_2822: Shew_2822 - polynucleotide phosphorylase/polyadenylase (RefSeq), at 3,343,826 to 3,345,928
_2822
Shew_2823: Shew_2823 - diguanylate cyclase/phosphodiesterase (RefSeq), at 3,346,124 to 3,348,157
_2823
Shew_2824: rpsO - 30S ribosomal protein S15 (RefSeq), at 3,348,265 to 3,348,534
rpsO
Shew_2825: Shew_2825 - tRNA pseudouridine synthase B (RefSeq), at 3,348,630 to 3,349,577
_2825
Position (kb)
3346
3347
3348
3349 Strain fitness (log2 ratio)
-1
0
1 at 3346.108 kb on + strand at 3346.502 kb on - strand, within Shew_2823 at 3346.633 kb on + strand, within Shew_2823 at 3347.016 kb on + strand, within Shew_2823 at 3347.217 kb on + strand, within Shew_2823 at 3347.225 kb on - strand, within Shew_2823 at 3347.316 kb on - strand, within Shew_2823 at 3347.735 kb on + strand, within Shew_2823 at 3347.815 kb on + strand, within Shew_2823 at 3347.815 kb on + strand, within Shew_2823 at 3347.815 kb on + strand, within Shew_2823 at 3348.775 kb on + strand, within Shew_2825 at 3349.000 kb on + strand, within Shew_2825 at 3349.095 kb on - strand, within Shew_2825
Per-strain Table
Position Strand Gene LocusTag Fraction D,L-Lactate (C) remove 3,346,108 + +1.1 3,346,502 - Shew_2823 0.19 +0.7 3,346,633 + Shew_2823 0.25 +0.5 3,347,016 + Shew_2823 0.44 +1.1 3,347,217 + Shew_2823 0.54 -1.5 3,347,225 - Shew_2823 0.54 +0.8 3,347,316 - Shew_2823 0.59 +0.2 3,347,735 + Shew_2823 0.79 +0.5 3,347,815 + Shew_2823 0.83 +0.2 3,347,815 + Shew_2823 0.83 +0.4 3,347,815 + Shew_2823 0.83 +0.8 3,348,775 + Shew_2825 0.15 -0.9 3,349,000 + Shew_2825 0.39 +0.1 3,349,095 - Shew_2825 0.49 +1.0
Or see this region's nucleotide sequence