Experiment: D,L-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2161 and cpdB are separated by 305 nucleotides cpdB and Shew_2163 are separated by 123 nucleotides Shew_2163 and Shew_2164 are separated by 112 nucleotides
Shew_2161: Shew_2161 - peptidyl-dipeptidase Dcp (RefSeq), at 2,508,721 to 2,510,901
_2161
Shew_2162: cpdB - bifunctional 2',3'-cyclic nucleotide 2'-phosphodiesterase/3'-nucleotidase periplasmic precursor protein (RefSeq), at 2,511,207 to 2,513,291
cpdB
Shew_2163: Shew_2163 - hypothetical protein (RefSeq), at 2,513,415 to 2,514,017
_2163
Shew_2164: Shew_2164 - acyl-CoA dehydrogenase domain-containing protein (RefSeq), at 2,514,130 to 2,515,920
_2164
Position (kb)
2511
2512
2513
2514 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2510.258 kb on - strand, within Shew_2161 at 2510.879 kb on + strand at 2511.101 kb on + strand at 2511.109 kb on - strand at 2511.952 kb on - strand, within cpdB at 2512.021 kb on - strand, within cpdB at 2512.162 kb on + strand, within cpdB at 2512.322 kb on - strand, within cpdB at 2512.343 kb on + strand, within cpdB at 2512.494 kb on + strand, within cpdB at 2512.862 kb on + strand, within cpdB at 2513.165 kb on - strand at 2513.952 kb on + strand, within Shew_2163 at 2514.210 kb on - strand at 2514.291 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D,L-Lactate (C) remove 2,510,258 - Shew_2161 0.70 +0.1 2,510,879 + +0.5 2,511,101 + +0.9 2,511,109 - -1.3 2,511,952 - cpdB Shew_2162 0.36 -0.9 2,512,021 - cpdB Shew_2162 0.39 +0.6 2,512,162 + cpdB Shew_2162 0.46 -2.7 2,512,322 - cpdB Shew_2162 0.53 -0.8 2,512,343 + cpdB Shew_2162 0.54 -3.2 2,512,494 + cpdB Shew_2162 0.62 +0.3 2,512,862 + cpdB Shew_2162 0.79 +0.5 2,513,165 - +0.4 2,513,952 + Shew_2163 0.89 +0.4 2,514,210 - -0.7 2,514,291 + -1.6
Or see this region's nucleotide sequence