Experiment: D,L-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2095 and Shew_2096 are separated by 2 nucleotides Shew_2096 and Shew_2097 are separated by 422 nucleotides
Shew_2095: Shew_2095 - phosphomethylpyrimidine kinase (RefSeq), at 2,421,161 to 2,422,906
_2095
Shew_2096: Shew_2096 - thiamine biosynthesis protein ThiC (RefSeq), at 2,422,909 to 2,424,960
_2096
Shew_2097: Shew_2097 - hypothetical protein (RefSeq), at 2,425,383 to 2,426,567
_2097
Position (kb)
2422
2423
2424
2425 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2422.252 kb on - strand, within Shew_2095 at 2422.306 kb on + strand, within Shew_2095 at 2422.319 kb on - strand, within Shew_2095 at 2422.388 kb on - strand, within Shew_2095 at 2422.398 kb on - strand, within Shew_2095 at 2422.408 kb on + strand, within Shew_2095 at 2422.593 kb on + strand, within Shew_2095 at 2422.663 kb on + strand, within Shew_2095 at 2422.663 kb on + strand, within Shew_2095 at 2422.663 kb on + strand, within Shew_2095 at 2423.079 kb on - strand at 2423.192 kb on - strand, within Shew_2096 at 2423.192 kb on - strand, within Shew_2096 at 2424.059 kb on + strand, within Shew_2096 at 2424.482 kb on - strand, within Shew_2096 at 2424.748 kb on + strand, within Shew_2096 at 2424.748 kb on + strand, within Shew_2096 at 2425.031 kb on + strand at 2425.241 kb on + strand at 2425.241 kb on + strand at 2425.241 kb on + strand at 2425.249 kb on - strand at 2425.249 kb on - strand at 2425.400 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D,L-Lactate (C) remove 2,422,252 - Shew_2095 0.62 +0.3 2,422,306 + Shew_2095 0.66 +0.3 2,422,319 - Shew_2095 0.66 +0.5 2,422,388 - Shew_2095 0.70 +0.0 2,422,398 - Shew_2095 0.71 -0.1 2,422,408 + Shew_2095 0.71 -3.5 2,422,593 + Shew_2095 0.82 -0.2 2,422,663 + Shew_2095 0.86 -1.2 2,422,663 + Shew_2095 0.86 +0.4 2,422,663 + Shew_2095 0.86 +0.2 2,423,079 - +0.3 2,423,192 - Shew_2096 0.14 +1.1 2,423,192 - Shew_2096 0.14 -1.9 2,424,059 + Shew_2096 0.56 +0.4 2,424,482 - Shew_2096 0.77 -0.9 2,424,748 + Shew_2096 0.90 +0.4 2,424,748 + Shew_2096 0.90 -0.6 2,425,031 + +0.6 2,425,241 + -0.9 2,425,241 + +0.8 2,425,241 + -0.6 2,425,249 - +0.2 2,425,249 - +0.4 2,425,400 + +0.3
Or see this region's nucleotide sequence