Experiment: D,L-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1757 and Shew_1758 overlap by 4 nucleotides Shew_1758 and Shew_1759 are separated by 2 nucleotides Shew_1759 and Shew_1760 are separated by 151 nucleotides
Shew_1757: Shew_1757 - hydrogenase expression/formation protein HypD (RefSeq), at 2,007,954 to 2,009,069
_1757
Shew_1758: Shew_1758 - hydrogenase assembly chaperone hypC/hupF (RefSeq), at 2,009,066 to 2,009,314
_1758
Shew_1759: Shew_1759 - hydrogenase accessory protein HypB (RefSeq), at 2,009,317 to 2,010,150
_1759
Shew_1760: Shew_1760 - (NiFe) hydrogenase maturation protein HypF (RefSeq), at 2,010,302 to 2,012,647
_1760
Position (kb)
2009
2010
2011 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2008.346 kb on + strand, within Shew_1757 at 2009.124 kb on - strand, within Shew_1758 at 2009.148 kb on + strand, within Shew_1758 at 2009.414 kb on + strand, within Shew_1759 at 2009.551 kb on + strand, within Shew_1759 at 2009.561 kb on + strand, within Shew_1759 at 2009.569 kb on - strand, within Shew_1759 at 2009.632 kb on + strand, within Shew_1759 at 2009.637 kb on + strand, within Shew_1759 at 2010.021 kb on + strand, within Shew_1759 at 2010.021 kb on + strand, within Shew_1759 at 2010.390 kb on - strand at 2011.049 kb on - strand, within Shew_1760 at 2011.061 kb on + strand, within Shew_1760 at 2011.140 kb on - strand, within Shew_1760
Per-strain Table
Position Strand Gene LocusTag Fraction D,L-Lactate (C) remove 2,008,346 + Shew_1757 0.35 -1.2 2,009,124 - Shew_1758 0.23 +0.1 2,009,148 + Shew_1758 0.33 +0.9 2,009,414 + Shew_1759 0.12 +0.9 2,009,551 + Shew_1759 0.28 +3.8 2,009,561 + Shew_1759 0.29 -0.8 2,009,569 - Shew_1759 0.30 +0.2 2,009,632 + Shew_1759 0.38 -2.1 2,009,637 + Shew_1759 0.38 +1.2 2,010,021 + Shew_1759 0.84 +1.0 2,010,021 + Shew_1759 0.84 -2.9 2,010,390 - -0.5 2,011,049 - Shew_1760 0.32 -1.7 2,011,061 + Shew_1760 0.32 +1.6 2,011,140 - Shew_1760 0.36 -0.0
Or see this region's nucleotide sequence