Experiment: D,L-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1510 and Shew_1511 are separated by 221 nucleotides Shew_1511 and Shew_1512 are separated by 260 nucleotides Shew_1512 and Shew_1513 overlap by 1 nucleotides
Shew_1510: Shew_1510 - peptidase M28 (RefSeq), at 1,732,190 to 1,733,851
_1510
Shew_1511: Shew_1511 - UMP phosphatase (RefSeq), at 1,734,073 to 1,734,831
_1511
Shew_1512: Shew_1512 - phosphoribosylglycinamide formyltransferase (RefSeq), at 1,735,092 to 1,735,736
_1512
Shew_1513: Shew_1513 - phosphoribosylaminoimidazole synthetase (RefSeq), at 1,735,736 to 1,736,773
_1513
Position (kb) 1734
1735 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 1733.286 kb on - strand, within Shew_1510 at 1733.816 kb on + strand at 1733.832 kb on - strand at 1734.148 kb on - strand at 1734.290 kb on + strand, within Shew_1511 at 1734.367 kb on + strand, within Shew_1511 at 1734.939 kb on - strand at 1735.002 kb on - strand at 1735.172 kb on + strand, within Shew_1512 at 1735.666 kb on - strand, within Shew_1512
Per-strain Table
Position Strand Gene LocusTag Fraction D,L-Lactate (C) remove 1,733,286 - Shew_1510 0.66 -0.3 1,733,816 + +0.3 1,733,832 - -0.7 1,734,148 - +0.1 1,734,290 + Shew_1511 0.29 -0.1 1,734,367 + Shew_1511 0.39 -0.6 1,734,939 - +0.2 1,735,002 - -2.4 1,735,172 + Shew_1512 0.12 -3.4 1,735,666 - Shew_1512 0.89 -6.0
Or see this region's nucleotide sequence