Experiment: D,L-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1446 and Shew_1447 are separated by 263 nucleotides Shew_1447 and Shew_1448 are separated by 108 nucleotides Shew_1448 and Shew_R0034 are separated by 152 nucleotides Shew_R0034 and Shew_R0035 are separated by 38 nucleotides Shew_R0035 and Shew_R0036 are separated by 37 nucleotides Shew_R0036 and Shew_R0037 are separated by 38 nucleotides Shew_R0037 and Shew_R0038 are separated by 59 nucleotides
Shew_1446: Shew_1446 - dicarboxylate TRAP transporter (succinate, fumarate, L-malate, and alpha-ketoglutarate), solute receptor component (from data) , at 1,662,719 to 1,663,729
_1446
Shew_1447: Shew_1447 - major facilitator transporter (RefSeq), at 1,663,993 to 1,665,258
_1447
Shew_1448: Shew_1448 - hypothetical protein (RefSeq), at 1,665,367 to 1,665,594
_1448
Shew_R0034: Shew_R0034 - tRNA-Val (RefSeq), at 1,665,747 to 1,665,822
_R0034
Shew_R0035: Shew_R0035 - tRNA-Val (RefSeq), at 1,665,861 to 1,665,936
_R0035
Shew_R0036: Shew_R0036 - tRNA-Val (RefSeq), at 1,665,974 to 1,666,049
_R0036
Shew_R0037: Shew_R0037 - tRNA-Val (RefSeq), at 1,666,088 to 1,666,163
_R0037
Shew_R0038: Shew_R0038 - tRNA-Val (RefSeq), at 1,666,223 to 1,666,298
_R0038
Position (kb)
1663
1664
1665
1666 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1662.997 kb on - strand, within Shew_1446 at 1663.025 kb on - strand, within Shew_1446 at 1663.037 kb on + strand, within Shew_1446 at 1663.186 kb on + strand, within Shew_1446 at 1663.770 kb on - strand at 1663.916 kb on - strand at 1664.199 kb on - strand, within Shew_1447 at 1664.355 kb on + strand, within Shew_1447 at 1664.493 kb on - strand, within Shew_1447 at 1664.693 kb on + strand, within Shew_1447 at 1664.954 kb on - strand, within Shew_1447 at 1664.966 kb on - strand, within Shew_1447 at 1664.966 kb on + strand, within Shew_1447 at 1665.078 kb on - strand, within Shew_1447 at 1665.113 kb on + strand, within Shew_1447 at 1665.188 kb on - strand at 1665.579 kb on + strand at 1665.690 kb on - strand at 1665.695 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D,L-Lactate (C) remove 1,662,997 - Shew_1446 0.27 +0.1 1,663,025 - Shew_1446 0.30 +0.1 1,663,037 + Shew_1446 0.31 +0.0 1,663,186 + Shew_1446 0.46 -0.4 1,663,770 - -0.2 1,663,916 - +0.0 1,664,199 - Shew_1447 0.16 -0.5 1,664,355 + Shew_1447 0.29 -2.9 1,664,493 - Shew_1447 0.39 +1.5 1,664,693 + Shew_1447 0.55 -0.3 1,664,954 - Shew_1447 0.76 +0.7 1,664,966 - Shew_1447 0.77 -2.4 1,664,966 + Shew_1447 0.77 -1.3 1,665,078 - Shew_1447 0.86 -0.1 1,665,113 + Shew_1447 0.88 -1.3 1,665,188 - -0.2 1,665,579 + +1.1 1,665,690 - -0.3 1,665,695 + +0.4
Or see this region's nucleotide sequence