Experiment: D,L-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1375 and fliP overlap by 4 nucleotides fliP and Shew_1377 are separated by 8 nucleotides Shew_1377 and fliR are separated by 42 nucleotides fliR and flhB overlap by 1 nucleotides
Shew_1375: Shew_1375 - flagellar biosynthesis protein, FliO (RefSeq), at 1,584,774 to 1,585,151
_1375
Shew_1376: fliP - flagellar biosynthesis protein FliP (RefSeq), at 1,585,148 to 1,585,915
fliP
Shew_1377: Shew_1377 - flagellar biosynthetic protein FliQ (RefSeq), at 1,585,924 to 1,586,193
_1377
Shew_1378: fliR - flagellar biosynthesis protein FliR (RefSeq), at 1,586,236 to 1,587,033
fliR
Shew_1379: flhB - flagellar biosynthesis protein FlhB (RefSeq), at 1,587,033 to 1,588,166
flhB
Position (kb) 1585
1586
1587 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1584.959 kb on + strand, within Shew_1375 at 1585.373 kb on - strand, within fliP at 1585.514 kb on - strand, within fliP at 1585.542 kb on + strand, within fliP at 1585.603 kb on - strand, within fliP at 1587.009 kb on + strand at 1587.064 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D,L-Lactate (C) remove 1,584,959 + Shew_1375 0.49 +1.5 1,585,373 - fliP Shew_1376 0.29 -2.1 1,585,514 - fliP Shew_1376 0.48 +1.6 1,585,542 + fliP Shew_1376 0.51 +1.4 1,585,603 - fliP Shew_1376 0.59 +2.5 1,587,009 + -0.1 1,587,064 - +1.7
Or see this region's nucleotide sequence