Experiment: D,L-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0288 and Shew_0289 are separated by 508 nucleotides Shew_0289 and ilvM overlap by 4 nucleotides ilvM and Shew_0291 are separated by 26 nucleotides
Shew_0288: Shew_0288 - ketol-acid reductoisomerase (RefSeq), at 306,553 to 308,034
_0288
Shew_0289: Shew_0289 - acetolactate synthase 2 catalytic subunit (RefSeq), at 308,543 to 310,216
_0289
Shew_0290: ilvM - acetolactate synthase 2 regulatory subunit (RefSeq), at 310,213 to 310,482
ilvM
Shew_0291: Shew_0291 - branched-chain amino acid aminotransferase (RefSeq), at 310,509 to 311,432
_0291
Position (kb) 308
309
310
311 Strain fitness (log2 ratio) -8
-7
-6
-5
-4
-3
-2
-1
0
1 at 307.725 kb on + strand, within Shew_0288 at 307.827 kb on + strand, within Shew_0288 at 307.846 kb on - strand, within Shew_0288 at 307.982 kb on - strand at 308.111 kb on + strand at 308.111 kb on + strand at 308.119 kb on - strand at 308.334 kb on + strand at 308.540 kb on - strand at 308.650 kb on + strand at 308.702 kb on - strand at 308.867 kb on + strand, within Shew_0289 at 308.965 kb on - strand, within Shew_0289 at 309.105 kb on + strand, within Shew_0289 at 309.105 kb on + strand, within Shew_0289 at 309.262 kb on + strand, within Shew_0289 at 309.272 kb on + strand, within Shew_0289 at 309.272 kb on + strand, within Shew_0289 at 309.338 kb on + strand, within Shew_0289 at 309.346 kb on - strand, within Shew_0289 at 309.543 kb on + strand, within Shew_0289 at 309.697 kb on + strand, within Shew_0289 at 309.730 kb on + strand, within Shew_0289 at 309.763 kb on - strand, within Shew_0289 at 309.820 kb on - strand, within Shew_0289 at 310.036 kb on - strand, within Shew_0289 at 310.141 kb on + strand at 310.300 kb on - strand, within ilvM at 310.347 kb on + strand, within ilvM at 310.655 kb on + strand, within Shew_0291 at 311.098 kb on + strand, within Shew_0291 at 311.106 kb on - strand, within Shew_0291 at 311.129 kb on + strand, within Shew_0291 at 311.137 kb on - strand, within Shew_0291 at 311.137 kb on - strand, within Shew_0291 at 311.137 kb on - strand, within Shew_0291 at 311.137 kb on - strand, within Shew_0291
Per-strain Table
Position Strand Gene LocusTag Fraction D,L-Lactate (C) remove 307,725 + Shew_0288 0.79 -4.9 307,827 + Shew_0288 0.86 -7.0 307,846 - Shew_0288 0.87 -5.5 307,982 - -4.3 308,111 + +0.2 308,111 + +1.0 308,119 - +0.6 308,334 + -3.9 308,540 - -7.8 308,650 + -4.9 308,702 - -7.1 308,867 + Shew_0289 0.19 -1.4 308,965 - Shew_0289 0.25 -1.8 309,105 + Shew_0289 0.34 -7.8 309,105 + Shew_0289 0.34 -1.9 309,262 + Shew_0289 0.43 -1.9 309,272 + Shew_0289 0.44 -0.3 309,272 + Shew_0289 0.44 -1.0 309,338 + Shew_0289 0.47 -0.8 309,346 - Shew_0289 0.48 -6.6 309,543 + Shew_0289 0.60 -2.5 309,697 + Shew_0289 0.69 -1.1 309,730 + Shew_0289 0.71 -0.8 309,763 - Shew_0289 0.73 -6.7 309,820 - Shew_0289 0.76 -3.1 310,036 - Shew_0289 0.89 -4.3 310,141 + -0.3 310,300 - ilvM Shew_0290 0.32 -4.1 310,347 + ilvM Shew_0290 0.50 -0.7 310,655 + Shew_0291 0.16 -0.8 311,098 + Shew_0291 0.64 -1.8 311,106 - Shew_0291 0.65 -7.0 311,129 + Shew_0291 0.67 -2.0 311,137 - Shew_0291 0.68 -4.9 311,137 - Shew_0291 0.68 -5.5 311,137 - Shew_0291 0.68 -7.1 311,137 - Shew_0291 0.68 -3.0
Or see this region's nucleotide sequence