Experiment: D,L-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0183 and rplQ are separated by 39 nucleotides rplQ and Shew_0185 are separated by 56 nucleotides Shew_0185 and Shew_0186 are separated by 134 nucleotides Shew_0186 and Shew_0187 overlap by 4 nucleotides Shew_0187 and Shew_0188 overlap by 1 nucleotides
Shew_0183: Shew_0183 - DNA-directed RNA polymerase subunit alpha (RefSeq), at 191,415 to 192,404
_0183
Shew_0184: rplQ - 50S ribosomal protein L17 (RefSeq), at 192,444 to 192,836
rplQ
Shew_0185: Shew_0185 - hypothetical protein (RefSeq), at 192,893 to 193,870
_0185
Shew_0186: Shew_0186 - cytochrome c-type biogenesis protein CcmE (RefSeq), at 194,005 to 194,490
_0186
Shew_0187: Shew_0187 - Heme exporter protein D (CcmD) (RefSeq), at 194,487 to 194,687
_0187
Shew_0188: Shew_0188 - heme exporter protein CcmC (RefSeq), at 194,687 to 195,433
_0188
Position (kb) 192
193
194 Strain fitness (log2 ratio) -1
0
1
2 at 193.143 kb on + strand, within Shew_0185 at 193.149 kb on - strand, within Shew_0185 at 193.440 kb on - strand, within Shew_0185 at 193.458 kb on + strand, within Shew_0185 at 193.466 kb on - strand, within Shew_0185 at 193.772 kb on - strand, within Shew_0185 at 193.974 kb on + strand at 194.030 kb on - strand at 194.050 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D,L-Lactate (C) remove 193,143 + Shew_0185 0.26 -0.1 193,149 - Shew_0185 0.26 +0.3 193,440 - Shew_0185 0.56 +0.5 193,458 + Shew_0185 0.58 +0.9 193,466 - Shew_0185 0.59 +0.3 193,772 - Shew_0185 0.90 +1.2 193,974 + +2.4 194,030 - +0.2 194,050 + -1.6
Or see this region's nucleotide sequence