Experiment: CDM1 without Valine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS19310 and BBR_RS19315 are separated by 180 nucleotides BBR_RS19315 and BBR_RS19320 are separated by 37 nucleotides BBR_RS19320 and BBR_RS19325 overlap by 11 nucleotides BBR_RS19325 and BBR_RS21085 overlap by 4 nucleotides BBR_RS21085 and BBR_RS19330 are separated by 50 nucleotides BBR_RS19330 and BBR_RS19335 are separated by 18 nucleotides
BBR_RS19310: BBR_RS19310 - MFS transporter, at 2,111,275 to 2,112,576
_RS19310
BBR_RS19315: BBR_RS19315 - Transcriptional regulator, MerR family, at 2,112,757 to 2,113,329
_RS19315
BBR_RS19320: BBR_RS19320 - hypothetical protein, at 2,113,367 to 2,113,756
_RS19320
BBR_RS19325: BBR_RS19325 - hypothetical protein, at 2,113,746 to 2,114,069
_RS19325
BBR_RS21085: BBR_RS21085 - hypothetical protein, at 2,114,066 to 2,114,278
_RS21085
BBR_RS19330: BBR_RS19330 - hypothetical protein, at 2,114,329 to 2,114,514
_RS19330
BBR_RS19335: BBR_RS19335 - helix-turn-helix domain-containing protein, at 2,114,533 to 2,115,603
_RS19335
Position (kb)
2113
2114 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2112.410 kb on - strand, within BBR_RS19310 at 2112.418 kb on - strand, within BBR_RS19310 at 2112.422 kb on - strand, within BBR_RS19310 at 2112.457 kb on - strand at 2112.473 kb on + strand at 2112.475 kb on - strand at 2112.502 kb on - strand at 2112.510 kb on - strand at 2112.520 kb on + strand at 2112.526 kb on + strand at 2112.534 kb on - strand at 2112.534 kb on - strand at 2112.542 kb on + strand at 2112.563 kb on - strand at 2112.578 kb on - strand at 2112.578 kb on - strand at 2112.578 kb on - strand at 2112.578 kb on - strand at 2112.579 kb on - strand at 2112.605 kb on - strand at 2112.611 kb on - strand at 2112.614 kb on + strand at 2112.635 kb on - strand at 2112.635 kb on - strand at 2112.639 kb on - strand at 2112.658 kb on + strand at 2112.663 kb on + strand at 2112.666 kb on - strand at 2112.671 kb on - strand at 2112.674 kb on - strand at 2112.676 kb on - strand at 2112.676 kb on - strand at 2112.680 kb on + strand at 2112.688 kb on - strand at 2112.688 kb on - strand at 2112.768 kb on + strand at 2112.768 kb on + strand at 2112.768 kb on + strand at 2112.775 kb on + strand at 2112.776 kb on - strand at 2112.776 kb on - strand at 2112.776 kb on - strand at 2112.776 kb on - strand at 2112.783 kb on + strand at 2112.791 kb on + strand at 2112.792 kb on - strand at 2112.803 kb on + strand at 2112.805 kb on + strand at 2112.819 kb on + strand, within BBR_RS19315 at 2112.827 kb on - strand, within BBR_RS19315 at 2112.847 kb on + strand, within BBR_RS19315 at 2112.877 kb on - strand, within BBR_RS19315 at 2112.887 kb on + strand, within BBR_RS19315 at 2112.887 kb on + strand, within BBR_RS19315 at 2112.895 kb on - strand, within BBR_RS19315 at 2112.895 kb on - strand, within BBR_RS19315 at 2112.908 kb on + strand, within BBR_RS19315 at 2112.948 kb on + strand, within BBR_RS19315 at 2112.973 kb on + strand, within BBR_RS19315 at 2112.981 kb on - strand, within BBR_RS19315 at 2113.079 kb on + strand, within BBR_RS19315 at 2113.087 kb on + strand, within BBR_RS19315 at 2113.087 kb on - strand, within BBR_RS19315 at 2113.095 kb on - strand, within BBR_RS19315 at 2113.116 kb on - strand, within BBR_RS19315 at 2113.143 kb on - strand, within BBR_RS19315 at 2113.151 kb on + strand, within BBR_RS19315 at 2113.171 kb on + strand, within BBR_RS19315 at 2113.181 kb on - strand, within BBR_RS19315 at 2113.196 kb on + strand, within BBR_RS19315 at 2113.207 kb on - strand, within BBR_RS19315 at 2113.220 kb on + strand, within BBR_RS19315 at 2113.232 kb on + strand, within BBR_RS19315 at 2113.289 kb on + strand at 2113.289 kb on + strand at 2113.298 kb on + strand at 2113.301 kb on - strand at 2113.316 kb on + strand at 2113.323 kb on + strand at 2113.331 kb on - strand at 2113.338 kb on + strand at 2113.338 kb on + strand at 2113.338 kb on - strand at 2113.346 kb on + strand at 2113.359 kb on + strand at 2113.359 kb on + strand at 2113.389 kb on + strand at 2113.389 kb on + strand at 2113.397 kb on - strand at 2113.412 kb on + strand, within BBR_RS19320 at 2113.419 kb on + strand, within BBR_RS19320 at 2113.420 kb on - strand, within BBR_RS19320 at 2113.420 kb on - strand, within BBR_RS19320 at 2113.420 kb on - strand, within BBR_RS19320 at 2113.433 kb on - strand, within BBR_RS19320 at 2113.465 kb on - strand, within BBR_RS19320 at 2113.465 kb on - strand, within BBR_RS19320 at 2113.465 kb on - strand, within BBR_RS19320 at 2113.505 kb on + strand, within BBR_RS19320 at 2113.528 kb on + strand, within BBR_RS19320 at 2113.528 kb on + strand, within BBR_RS19320 at 2113.528 kb on + strand, within BBR_RS19320 at 2113.536 kb on - strand, within BBR_RS19320 at 2113.536 kb on - strand, within BBR_RS19320 at 2113.536 kb on - strand, within BBR_RS19320 at 2113.541 kb on - strand, within BBR_RS19320 at 2113.549 kb on - strand, within BBR_RS19320 at 2113.558 kb on + strand, within BBR_RS19320 at 2113.558 kb on + strand, within BBR_RS19320 at 2113.559 kb on - strand, within BBR_RS19320 at 2113.566 kb on - strand, within BBR_RS19320 at 2113.566 kb on - strand, within BBR_RS19320 at 2113.566 kb on - strand, within BBR_RS19320 at 2113.566 kb on - strand, within BBR_RS19320 at 2113.570 kb on - strand, within BBR_RS19320 at 2113.593 kb on - strand, within BBR_RS19320 at 2113.603 kb on - strand, within BBR_RS19320 at 2113.626 kb on - strand, within BBR_RS19320 at 2113.642 kb on - strand, within BBR_RS19320 at 2113.642 kb on - strand, within BBR_RS19320 at 2113.644 kb on - strand, within BBR_RS19320 at 2113.644 kb on - strand, within BBR_RS19320 at 2113.644 kb on - strand, within BBR_RS19320 at 2113.644 kb on - strand, within BBR_RS19320 at 2113.644 kb on - strand, within BBR_RS19320 at 2113.644 kb on - strand, within BBR_RS19320 at 2113.673 kb on - strand, within BBR_RS19320 at 2113.686 kb on - strand, within BBR_RS19320 at 2113.747 kb on - strand at 2113.759 kb on + strand at 2113.765 kb on + strand at 2113.765 kb on + strand at 2113.765 kb on + strand at 2113.773 kb on - strand at 2113.773 kb on - strand at 2113.773 kb on - strand at 2113.778 kb on - strand at 2113.807 kb on - strand, within BBR_RS19325 at 2113.945 kb on - strand, within BBR_RS19325 at 2113.947 kb on - strand, within BBR_RS19325 at 2113.951 kb on + strand, within BBR_RS19325 at 2113.958 kb on + strand, within BBR_RS19325 at 2114.015 kb on - strand, within BBR_RS19325 at 2114.062 kb on - strand at 2114.168 kb on + strand, within BBR_RS21085 at 2114.168 kb on + strand, within BBR_RS21085 at 2114.189 kb on + strand, within BBR_RS21085 at 2114.209 kb on - strand, within BBR_RS21085 at 2114.225 kb on + strand, within BBR_RS21085 at 2114.230 kb on + strand, within BBR_RS21085 at 2114.230 kb on + strand, within BBR_RS21085 at 2114.247 kb on + strand, within BBR_RS21085 at 2114.247 kb on + strand, within BBR_RS21085 at 2114.261 kb on + strand at 2114.271 kb on + strand at 2114.271 kb on + strand at 2114.271 kb on + strand at 2114.279 kb on - strand at 2114.279 kb on - strand at 2114.279 kb on - strand at 2114.279 kb on - strand at 2114.279 kb on - strand at 2114.279 kb on - strand at 2114.281 kb on - strand at 2114.289 kb on - strand at 2114.333 kb on + strand at 2114.349 kb on - strand, within BBR_RS19330 at 2114.385 kb on + strand, within BBR_RS19330 at 2114.408 kb on + strand, within BBR_RS19330 at 2114.408 kb on - strand, within BBR_RS19330 at 2114.416 kb on - strand, within BBR_RS19330 at 2114.416 kb on - strand, within BBR_RS19330 at 2114.455 kb on + strand, within BBR_RS19330 at 2114.470 kb on + strand, within BBR_RS19330 at 2114.473 kb on - strand, within BBR_RS19330 at 2114.478 kb on - strand, within BBR_RS19330 at 2114.478 kb on - strand, within BBR_RS19330 at 2114.488 kb on - strand, within BBR_RS19330 at 2114.508 kb on + strand at 2114.584 kb on + strand at 2114.597 kb on - strand at 2114.612 kb on + strand at 2114.612 kb on + strand at 2114.617 kb on + strand at 2114.617 kb on + strand at 2114.620 kb on - strand at 2114.620 kb on - strand at 2114.620 kb on - strand at 2114.620 kb on - strand at 2114.622 kb on + strand at 2114.630 kb on - strand at 2114.630 kb on - strand at 2114.653 kb on - strand, within BBR_RS19335 at 2114.655 kb on + strand, within BBR_RS19335 at 2114.658 kb on - strand, within BBR_RS19335 at 2114.658 kb on - strand, within BBR_RS19335 at 2114.663 kb on + strand, within BBR_RS19335 at 2114.683 kb on - strand, within BBR_RS19335 at 2114.688 kb on - strand, within BBR_RS19335 at 2114.692 kb on - strand, within BBR_RS19335 at 2114.752 kb on + strand, within BBR_RS19335
Per-strain Table
Position Strand Gene LocusTag Fraction CDM1 without Valine remove 2,112,410 - BBR_RS19310 0.87 -0.2 2,112,418 - BBR_RS19310 0.88 -0.4 2,112,422 - BBR_RS19310 0.88 +0.0 2,112,457 - +1.1 2,112,473 + -1.3 2,112,475 - -1.1 2,112,502 - -0.2 2,112,510 - -0.7 2,112,520 + +0.1 2,112,526 + +0.6 2,112,534 - -0.5 2,112,534 - +0.3 2,112,542 + +0.2 2,112,563 - -0.4 2,112,578 - -0.9 2,112,578 - -0.0 2,112,578 - +0.7 2,112,578 - +0.5 2,112,579 - +0.3 2,112,605 - +1.2 2,112,611 - +0.3 2,112,614 + +1.0 2,112,635 - -0.4 2,112,635 - -0.9 2,112,639 - -0.3 2,112,658 + +0.3 2,112,663 + -1.1 2,112,666 - -1.0 2,112,671 - +0.3 2,112,674 - -0.3 2,112,676 - +0.9 2,112,676 - -0.4 2,112,680 + -0.6 2,112,688 - -1.0 2,112,688 - -0.0 2,112,768 + -0.8 2,112,768 + +0.9 2,112,768 + -0.3 2,112,775 + -0.9 2,112,776 - -0.1 2,112,776 - +0.5 2,112,776 - +0.4 2,112,776 - -2.0 2,112,783 + +0.0 2,112,791 + +0.6 2,112,792 - +0.1 2,112,803 + -0.4 2,112,805 + +0.2 2,112,819 + BBR_RS19315 0.11 -2.0 2,112,827 - BBR_RS19315 0.12 -0.2 2,112,847 + BBR_RS19315 0.16 -1.3 2,112,877 - BBR_RS19315 0.21 +0.6 2,112,887 + BBR_RS19315 0.23 -2.4 2,112,887 + BBR_RS19315 0.23 -1.9 2,112,895 - BBR_RS19315 0.24 +0.4 2,112,895 - BBR_RS19315 0.24 -0.1 2,112,908 + BBR_RS19315 0.26 +0.3 2,112,948 + BBR_RS19315 0.33 -1.2 2,112,973 + BBR_RS19315 0.38 -0.2 2,112,981 - BBR_RS19315 0.39 -0.4 2,113,079 + BBR_RS19315 0.56 -0.1 2,113,087 + BBR_RS19315 0.58 -1.6 2,113,087 - BBR_RS19315 0.58 -0.1 2,113,095 - BBR_RS19315 0.59 -0.3 2,113,116 - BBR_RS19315 0.63 +0.7 2,113,143 - BBR_RS19315 0.67 +0.3 2,113,151 + BBR_RS19315 0.69 -2.2 2,113,171 + BBR_RS19315 0.72 -1.8 2,113,181 - BBR_RS19315 0.74 -2.8 2,113,196 + BBR_RS19315 0.77 -2.6 2,113,207 - BBR_RS19315 0.79 +0.7 2,113,220 + BBR_RS19315 0.81 -0.3 2,113,232 + BBR_RS19315 0.83 -0.9 2,113,289 + -1.8 2,113,289 + -0.5 2,113,298 + -2.5 2,113,301 - +0.5 2,113,316 + -0.8 2,113,323 + -0.9 2,113,331 - +0.0 2,113,338 + -1.6 2,113,338 + +0.2 2,113,338 - +0.8 2,113,346 + -0.9 2,113,359 + +0.4 2,113,359 + -0.0 2,113,389 + +1.3 2,113,389 + -0.4 2,113,397 - +0.1 2,113,412 + BBR_RS19320 0.12 -1.2 2,113,419 + BBR_RS19320 0.13 -0.5 2,113,420 - BBR_RS19320 0.14 -0.5 2,113,420 - BBR_RS19320 0.14 +0.7 2,113,420 - BBR_RS19320 0.14 +0.2 2,113,433 - BBR_RS19320 0.17 +0.4 2,113,465 - BBR_RS19320 0.25 +0.0 2,113,465 - BBR_RS19320 0.25 -1.1 2,113,465 - BBR_RS19320 0.25 +1.0 2,113,505 + BBR_RS19320 0.35 -0.2 2,113,528 + BBR_RS19320 0.41 -1.4 2,113,528 + BBR_RS19320 0.41 -2.5 2,113,528 + BBR_RS19320 0.41 -0.0 2,113,536 - BBR_RS19320 0.43 +0.5 2,113,536 - BBR_RS19320 0.43 +0.8 2,113,536 - BBR_RS19320 0.43 -0.5 2,113,541 - BBR_RS19320 0.45 -0.5 2,113,549 - BBR_RS19320 0.47 +0.2 2,113,558 + BBR_RS19320 0.49 -2.4 2,113,558 + BBR_RS19320 0.49 -0.7 2,113,559 - BBR_RS19320 0.49 +1.7 2,113,566 - BBR_RS19320 0.51 +0.6 2,113,566 - BBR_RS19320 0.51 +0.9 2,113,566 - BBR_RS19320 0.51 -0.8 2,113,566 - BBR_RS19320 0.51 +0.5 2,113,570 - BBR_RS19320 0.52 -1.1 2,113,593 - BBR_RS19320 0.58 -0.4 2,113,603 - BBR_RS19320 0.61 -0.4 2,113,626 - BBR_RS19320 0.66 +0.8 2,113,642 - BBR_RS19320 0.71 +1.2 2,113,642 - BBR_RS19320 0.71 -0.9 2,113,644 - BBR_RS19320 0.71 +1.5 2,113,644 - BBR_RS19320 0.71 +0.1 2,113,644 - BBR_RS19320 0.71 -0.1 2,113,644 - BBR_RS19320 0.71 -0.0 2,113,644 - BBR_RS19320 0.71 +0.6 2,113,644 - BBR_RS19320 0.71 +0.2 2,113,673 - BBR_RS19320 0.78 -1.5 2,113,686 - BBR_RS19320 0.82 +1.2 2,113,747 - -1.1 2,113,759 + -2.1 2,113,765 + -1.4 2,113,765 + -1.1 2,113,765 + -2.0 2,113,773 - -0.9 2,113,773 - -2.1 2,113,773 - -1.7 2,113,778 - -1.8 2,113,807 - BBR_RS19325 0.19 -2.4 2,113,945 - BBR_RS19325 0.61 -3.1 2,113,947 - BBR_RS19325 0.62 -1.4 2,113,951 + BBR_RS19325 0.63 -4.5 2,113,958 + BBR_RS19325 0.65 -1.7 2,114,015 - BBR_RS19325 0.83 +0.4 2,114,062 - +0.2 2,114,168 + BBR_RS21085 0.48 -0.6 2,114,168 + BBR_RS21085 0.48 -0.3 2,114,189 + BBR_RS21085 0.58 -0.5 2,114,209 - BBR_RS21085 0.67 -0.0 2,114,225 + BBR_RS21085 0.75 -1.1 2,114,230 + BBR_RS21085 0.77 -1.1 2,114,230 + BBR_RS21085 0.77 -0.0 2,114,247 + BBR_RS21085 0.85 -1.3 2,114,247 + BBR_RS21085 0.85 -0.6 2,114,261 + +0.1 2,114,271 + -0.6 2,114,271 + -0.1 2,114,271 + -0.5 2,114,279 - +0.2 2,114,279 - +0.8 2,114,279 - +0.3 2,114,279 - -3.0 2,114,279 - -0.2 2,114,279 - -0.4 2,114,281 - +0.0 2,114,289 - +0.5 2,114,333 + +0.7 2,114,349 - BBR_RS19330 0.11 -1.3 2,114,385 + BBR_RS19330 0.30 -0.1 2,114,408 + BBR_RS19330 0.42 -0.4 2,114,408 - BBR_RS19330 0.42 -0.3 2,114,416 - BBR_RS19330 0.47 -1.3 2,114,416 - BBR_RS19330 0.47 -1.3 2,114,455 + BBR_RS19330 0.68 +1.2 2,114,470 + BBR_RS19330 0.76 -1.1 2,114,473 - BBR_RS19330 0.77 +0.0 2,114,478 - BBR_RS19330 0.80 -0.9 2,114,478 - BBR_RS19330 0.80 -0.1 2,114,488 - BBR_RS19330 0.85 +0.5 2,114,508 + -1.1 2,114,584 + +0.0 2,114,597 - +0.8 2,114,612 + -0.3 2,114,612 + +0.0 2,114,617 + -1.1 2,114,617 + -2.2 2,114,620 - -0.6 2,114,620 - -0.5 2,114,620 - +0.3 2,114,620 - +0.9 2,114,622 + -0.6 2,114,630 - +0.8 2,114,630 - +0.0 2,114,653 - BBR_RS19335 0.11 +0.2 2,114,655 + BBR_RS19335 0.11 -0.4 2,114,658 - BBR_RS19335 0.12 +1.2 2,114,658 - BBR_RS19335 0.12 +1.1 2,114,663 + BBR_RS19335 0.12 -0.0 2,114,683 - BBR_RS19335 0.14 +0.2 2,114,688 - BBR_RS19335 0.14 +0.5 2,114,692 - BBR_RS19335 0.15 -0.2 2,114,752 + BBR_RS19335 0.20 -1.7
Or see this region's nucleotide sequence