Experiment: CDM1 without Valine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS17065 and BBR_RS17070 are separated by 138 nucleotides BBR_RS17070 and BBR_RS17075 are separated by 26 nucleotides BBR_RS17075 and BBR_RS17080 overlap by 4 nucleotides
BBR_RS17065: BBR_RS17065 - aldo/keto reductase, at 1,602,832 to 1,603,659
_RS17065
BBR_RS17070: BBR_RS17070 - hypothetical protein, at 1,603,798 to 1,606,149
_RS17070
BBR_RS17075: BBR_RS17075 - dihydrodipicolinate synthase family protein, at 1,606,176 to 1,607,069
_RS17075
BBR_RS17080: BBR_RS17080 - hypothetical protein, at 1,607,066 to 1,607,887
_RS17080
Position (kb)
1603
1604
1605
1606
1607 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1602.848 kb on + strand at 1602.875 kb on + strand at 1602.877 kb on - strand at 1602.890 kb on - strand at 1602.900 kb on - strand at 1602.938 kb on + strand, within BBR_RS17065 at 1602.986 kb on + strand, within BBR_RS17065 at 1603.115 kb on + strand, within BBR_RS17065 at 1603.123 kb on - strand, within BBR_RS17065 at 1603.132 kb on - strand, within BBR_RS17065 at 1603.191 kb on + strand, within BBR_RS17065 at 1603.197 kb on - strand, within BBR_RS17065 at 1603.232 kb on - strand, within BBR_RS17065 at 1603.301 kb on + strand, within BBR_RS17065 at 1603.332 kb on - strand, within BBR_RS17065 at 1603.364 kb on - strand, within BBR_RS17065 at 1603.383 kb on + strand, within BBR_RS17065 at 1603.391 kb on - strand, within BBR_RS17065 at 1603.425 kb on - strand, within BBR_RS17065 at 1603.425 kb on - strand, within BBR_RS17065 at 1603.425 kb on - strand, within BBR_RS17065 at 1603.425 kb on - strand, within BBR_RS17065 at 1603.425 kb on - strand, within BBR_RS17065 at 1603.427 kb on + strand, within BBR_RS17065 at 1603.461 kb on - strand, within BBR_RS17065 at 1603.495 kb on + strand, within BBR_RS17065 at 1603.503 kb on - strand, within BBR_RS17065 at 1603.523 kb on + strand, within BBR_RS17065 at 1603.541 kb on + strand, within BBR_RS17065 at 1603.561 kb on - strand, within BBR_RS17065 at 1603.590 kb on - strand at 1603.595 kb on - strand at 1603.595 kb on - strand at 1603.665 kb on + strand at 1603.739 kb on + strand at 1603.747 kb on - strand at 1603.857 kb on - strand at 1603.909 kb on + strand at 1603.915 kb on - strand at 1603.915 kb on - strand at 1603.936 kb on - strand at 1603.936 kb on - strand at 1603.996 kb on - strand at 1603.996 kb on - strand at 1604.002 kb on - strand at 1604.013 kb on - strand at 1604.013 kb on - strand at 1604.122 kb on + strand, within BBR_RS17070 at 1604.137 kb on - strand, within BBR_RS17070 at 1604.202 kb on + strand, within BBR_RS17070 at 1604.251 kb on + strand, within BBR_RS17070 at 1604.316 kb on - strand, within BBR_RS17070 at 1604.323 kb on - strand, within BBR_RS17070 at 1604.323 kb on - strand, within BBR_RS17070 at 1604.331 kb on - strand, within BBR_RS17070 at 1604.346 kb on - strand, within BBR_RS17070 at 1604.348 kb on + strand, within BBR_RS17070 at 1604.364 kb on - strand, within BBR_RS17070 at 1604.364 kb on - strand, within BBR_RS17070 at 1604.484 kb on - strand, within BBR_RS17070 at 1604.489 kb on - strand, within BBR_RS17070 at 1604.489 kb on - strand, within BBR_RS17070 at 1604.491 kb on - strand, within BBR_RS17070 at 1604.522 kb on - strand, within BBR_RS17070 at 1604.552 kb on - strand, within BBR_RS17070 at 1604.569 kb on - strand, within BBR_RS17070 at 1604.583 kb on - strand, within BBR_RS17070 at 1604.585 kb on + strand, within BBR_RS17070 at 1604.607 kb on + strand, within BBR_RS17070 at 1604.628 kb on + strand, within BBR_RS17070 at 1604.628 kb on + strand, within BBR_RS17070 at 1604.643 kb on + strand, within BBR_RS17070 at 1604.646 kb on - strand, within BBR_RS17070 at 1604.721 kb on - strand, within BBR_RS17070 at 1604.763 kb on + strand, within BBR_RS17070 at 1604.763 kb on + strand, within BBR_RS17070 at 1604.782 kb on - strand, within BBR_RS17070 at 1604.868 kb on + strand, within BBR_RS17070 at 1604.936 kb on - strand, within BBR_RS17070 at 1605.020 kb on + strand, within BBR_RS17070 at 1605.045 kb on - strand, within BBR_RS17070 at 1605.054 kb on - strand, within BBR_RS17070 at 1605.193 kb on + strand, within BBR_RS17070 at 1605.216 kb on + strand, within BBR_RS17070 at 1605.374 kb on - strand, within BBR_RS17070 at 1605.384 kb on + strand, within BBR_RS17070 at 1605.435 kb on - strand, within BBR_RS17070 at 1605.435 kb on - strand, within BBR_RS17070 at 1605.435 kb on - strand, within BBR_RS17070 at 1605.439 kb on + strand, within BBR_RS17070 at 1605.467 kb on - strand, within BBR_RS17070 at 1605.471 kb on + strand, within BBR_RS17070 at 1605.479 kb on - strand, within BBR_RS17070 at 1605.498 kb on - strand, within BBR_RS17070 at 1605.498 kb on - strand, within BBR_RS17070 at 1605.542 kb on + strand, within BBR_RS17070 at 1605.551 kb on - strand, within BBR_RS17070 at 1605.567 kb on + strand, within BBR_RS17070 at 1605.575 kb on + strand, within BBR_RS17070 at 1605.580 kb on + strand, within BBR_RS17070 at 1605.588 kb on - strand, within BBR_RS17070 at 1605.594 kb on + strand, within BBR_RS17070 at 1605.610 kb on + strand, within BBR_RS17070 at 1605.624 kb on + strand, within BBR_RS17070 at 1605.700 kb on + strand, within BBR_RS17070 at 1605.828 kb on + strand, within BBR_RS17070 at 1606.011 kb on - strand at 1606.014 kb on - strand at 1606.064 kb on + strand at 1606.067 kb on - strand at 1606.072 kb on - strand at 1606.084 kb on - strand at 1606.084 kb on - strand at 1606.084 kb on - strand at 1606.101 kb on - strand at 1606.101 kb on - strand at 1606.107 kb on + strand at 1606.159 kb on - strand at 1606.196 kb on - strand at 1606.196 kb on - strand at 1606.196 kb on - strand at 1606.223 kb on - strand at 1606.223 kb on - strand at 1606.263 kb on - strand at 1606.279 kb on + strand, within BBR_RS17075 at 1606.309 kb on + strand, within BBR_RS17075 at 1606.387 kb on - strand, within BBR_RS17075 at 1606.392 kb on - strand, within BBR_RS17075 at 1606.397 kb on - strand, within BBR_RS17075 at 1606.434 kb on - strand, within BBR_RS17075 at 1606.468 kb on + strand, within BBR_RS17075 at 1606.468 kb on + strand, within BBR_RS17075 at 1606.468 kb on - strand, within BBR_RS17075 at 1606.476 kb on - strand, within BBR_RS17075 at 1606.476 kb on - strand, within BBR_RS17075 at 1606.535 kb on + strand, within BBR_RS17075 at 1606.564 kb on + strand, within BBR_RS17075 at 1606.616 kb on + strand, within BBR_RS17075 at 1606.629 kb on + strand, within BBR_RS17075 at 1606.681 kb on + strand, within BBR_RS17075 at 1606.689 kb on - strand, within BBR_RS17075 at 1606.691 kb on + strand, within BBR_RS17075 at 1606.691 kb on + strand, within BBR_RS17075 at 1606.701 kb on + strand, within BBR_RS17075 at 1606.721 kb on + strand, within BBR_RS17075 at 1606.750 kb on - strand, within BBR_RS17075 at 1606.768 kb on - strand, within BBR_RS17075 at 1606.787 kb on + strand, within BBR_RS17075 at 1606.806 kb on + strand, within BBR_RS17075 at 1606.828 kb on - strand, within BBR_RS17075 at 1606.932 kb on + strand, within BBR_RS17075 at 1606.944 kb on + strand, within BBR_RS17075 at 1606.997 kb on + strand at 1607.005 kb on - strand at 1607.009 kb on - strand at 1607.049 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction CDM1 without Valine remove 1,602,848 + +0.4 1,602,875 + -0.4 1,602,877 - -0.1 1,602,890 - -0.5 1,602,900 - -1.1 1,602,938 + BBR_RS17065 0.13 +1.1 1,602,986 + BBR_RS17065 0.19 +0.4 1,603,115 + BBR_RS17065 0.34 -0.0 1,603,123 - BBR_RS17065 0.35 -1.3 1,603,132 - BBR_RS17065 0.36 -0.3 1,603,191 + BBR_RS17065 0.43 +0.9 1,603,197 - BBR_RS17065 0.44 -0.9 1,603,232 - BBR_RS17065 0.48 -0.1 1,603,301 + BBR_RS17065 0.57 -2.5 1,603,332 - BBR_RS17065 0.60 +1.0 1,603,364 - BBR_RS17065 0.64 -0.3 1,603,383 + BBR_RS17065 0.67 -1.7 1,603,391 - BBR_RS17065 0.68 -1.6 1,603,425 - BBR_RS17065 0.72 -1.6 1,603,425 - BBR_RS17065 0.72 -0.8 1,603,425 - BBR_RS17065 0.72 +0.2 1,603,425 - BBR_RS17065 0.72 -0.6 1,603,425 - BBR_RS17065 0.72 -0.6 1,603,427 + BBR_RS17065 0.72 -0.7 1,603,461 - BBR_RS17065 0.76 +1.0 1,603,495 + BBR_RS17065 0.80 -1.1 1,603,503 - BBR_RS17065 0.81 -1.9 1,603,523 + BBR_RS17065 0.83 -2.4 1,603,541 + BBR_RS17065 0.86 -0.3 1,603,561 - BBR_RS17065 0.88 +0.4 1,603,590 - -0.6 1,603,595 - +1.0 1,603,595 - -1.9 1,603,665 + +0.5 1,603,739 + -2.5 1,603,747 - -1.3 1,603,857 - +0.6 1,603,909 + +0.1 1,603,915 - -0.7 1,603,915 - +0.1 1,603,936 - +0.5 1,603,936 - -0.3 1,603,996 - +0.8 1,603,996 - +1.5 1,604,002 - +1.7 1,604,013 - +0.5 1,604,013 - +0.6 1,604,122 + BBR_RS17070 0.14 -0.9 1,604,137 - BBR_RS17070 0.14 +0.4 1,604,202 + BBR_RS17070 0.17 -0.5 1,604,251 + BBR_RS17070 0.19 -1.4 1,604,316 - BBR_RS17070 0.22 +0.1 1,604,323 - BBR_RS17070 0.22 +0.9 1,604,323 - BBR_RS17070 0.22 +1.9 1,604,331 - BBR_RS17070 0.23 +1.0 1,604,346 - BBR_RS17070 0.23 -1.0 1,604,348 + BBR_RS17070 0.23 +0.3 1,604,364 - BBR_RS17070 0.24 +1.2 1,604,364 - BBR_RS17070 0.24 -1.0 1,604,484 - BBR_RS17070 0.29 +2.5 1,604,489 - BBR_RS17070 0.29 +0.3 1,604,489 - BBR_RS17070 0.29 +1.5 1,604,491 - BBR_RS17070 0.29 +1.0 1,604,522 - BBR_RS17070 0.31 +2.1 1,604,552 - BBR_RS17070 0.32 +0.3 1,604,569 - BBR_RS17070 0.33 +0.3 1,604,583 - BBR_RS17070 0.33 +1.0 1,604,585 + BBR_RS17070 0.33 -1.4 1,604,607 + BBR_RS17070 0.34 -1.3 1,604,628 + BBR_RS17070 0.35 -1.3 1,604,628 + BBR_RS17070 0.35 +0.4 1,604,643 + BBR_RS17070 0.36 +0.9 1,604,646 - BBR_RS17070 0.36 +0.2 1,604,721 - BBR_RS17070 0.39 +0.2 1,604,763 + BBR_RS17070 0.41 -0.3 1,604,763 + BBR_RS17070 0.41 +1.2 1,604,782 - BBR_RS17070 0.42 +1.2 1,604,868 + BBR_RS17070 0.45 -0.0 1,604,936 - BBR_RS17070 0.48 +0.6 1,605,020 + BBR_RS17070 0.52 +0.3 1,605,045 - BBR_RS17070 0.53 +0.4 1,605,054 - BBR_RS17070 0.53 +0.8 1,605,193 + BBR_RS17070 0.59 +0.5 1,605,216 + BBR_RS17070 0.60 +2.2 1,605,374 - BBR_RS17070 0.67 +0.1 1,605,384 + BBR_RS17070 0.67 +1.7 1,605,435 - BBR_RS17070 0.70 -0.5 1,605,435 - BBR_RS17070 0.70 -1.0 1,605,435 - BBR_RS17070 0.70 -0.4 1,605,439 + BBR_RS17070 0.70 +0.3 1,605,467 - BBR_RS17070 0.71 +1.0 1,605,471 + BBR_RS17070 0.71 +0.3 1,605,479 - BBR_RS17070 0.71 -1.2 1,605,498 - BBR_RS17070 0.72 -0.8 1,605,498 - BBR_RS17070 0.72 +0.0 1,605,542 + BBR_RS17070 0.74 -1.5 1,605,551 - BBR_RS17070 0.75 +1.3 1,605,567 + BBR_RS17070 0.75 +0.4 1,605,575 + BBR_RS17070 0.76 -0.1 1,605,580 + BBR_RS17070 0.76 +0.5 1,605,588 - BBR_RS17070 0.76 -0.9 1,605,594 + BBR_RS17070 0.76 -1.1 1,605,610 + BBR_RS17070 0.77 +0.8 1,605,624 + BBR_RS17070 0.78 +0.3 1,605,700 + BBR_RS17070 0.81 +0.4 1,605,828 + BBR_RS17070 0.86 +1.3 1,606,011 - +1.0 1,606,014 - +1.9 1,606,064 + -0.7 1,606,067 - -0.7 1,606,072 - +0.8 1,606,084 - -0.1 1,606,084 - -0.3 1,606,084 - -0.3 1,606,101 - -2.8 1,606,101 - +0.6 1,606,107 + -1.4 1,606,159 - +0.2 1,606,196 - -0.5 1,606,196 - -0.0 1,606,196 - +0.2 1,606,223 - +1.5 1,606,223 - +0.2 1,606,263 - +0.4 1,606,279 + BBR_RS17075 0.12 +0.1 1,606,309 + BBR_RS17075 0.15 +1.1 1,606,387 - BBR_RS17075 0.24 +0.1 1,606,392 - BBR_RS17075 0.24 +0.2 1,606,397 - BBR_RS17075 0.25 +0.4 1,606,434 - BBR_RS17075 0.29 +0.7 1,606,468 + BBR_RS17075 0.33 -1.5 1,606,468 + BBR_RS17075 0.33 +0.7 1,606,468 - BBR_RS17075 0.33 -0.5 1,606,476 - BBR_RS17075 0.34 -0.0 1,606,476 - BBR_RS17075 0.34 +0.9 1,606,535 + BBR_RS17075 0.40 +1.0 1,606,564 + BBR_RS17075 0.43 -1.4 1,606,616 + BBR_RS17075 0.49 -1.7 1,606,629 + BBR_RS17075 0.51 -0.7 1,606,681 + BBR_RS17075 0.56 +0.2 1,606,689 - BBR_RS17075 0.57 +0.3 1,606,691 + BBR_RS17075 0.58 +1.1 1,606,691 + BBR_RS17075 0.58 +0.3 1,606,701 + BBR_RS17075 0.59 -1.9 1,606,721 + BBR_RS17075 0.61 -0.1 1,606,750 - BBR_RS17075 0.64 -0.3 1,606,768 - BBR_RS17075 0.66 -0.6 1,606,787 + BBR_RS17075 0.68 -1.0 1,606,806 + BBR_RS17075 0.70 -1.9 1,606,828 - BBR_RS17075 0.73 -0.3 1,606,932 + BBR_RS17075 0.85 +0.9 1,606,944 + BBR_RS17075 0.86 -2.8 1,606,997 + -0.5 1,607,005 - -0.4 1,607,009 - -0.2 1,607,049 + +0.7
Or see this region's nucleotide sequence