Experiment: CDM1 without Valine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS16065 and BBR_RS16070 are separated by 65 nucleotides BBR_RS16070 and BBR_RS16075 are separated by 317 nucleotides BBR_RS16075 and BBR_RS16080 overlap by 17 nucleotides BBR_RS16080 and BBR_RS16085 are separated by 362 nucleotides
BBR_RS16065: BBR_RS16065 - DUF488 domain-containing protein, at 1,355,853 to 1,356,206
_RS16065
BBR_RS16070: BBR_RS16070 - hypothetical protein, at 1,356,272 to 1,356,700
_RS16070
BBR_RS16075: BBR_RS16075 - hypothetical protein, at 1,357,018 to 1,357,914
_RS16075
BBR_RS16080: BBR_RS16080 - DUF1287 domain-containing protein, at 1,357,898 to 1,358,260
_RS16080
BBR_RS16085: BBR_RS16085 - anthranilate synthase component I, at 1,358,623 to 1,360,179
_RS16085
Position (kb)
1357
1358 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1356.114 kb on - strand, within BBR_RS16065 at 1356.149 kb on - strand, within BBR_RS16065 at 1356.166 kb on + strand, within BBR_RS16065 at 1356.174 kb on - strand at 1356.356 kb on + strand, within BBR_RS16070 at 1356.399 kb on + strand, within BBR_RS16070 at 1356.529 kb on - strand, within BBR_RS16070 at 1356.587 kb on + strand, within BBR_RS16070 at 1356.587 kb on + strand, within BBR_RS16070 at 1356.619 kb on + strand, within BBR_RS16070 at 1356.634 kb on + strand, within BBR_RS16070 at 1356.736 kb on + strand at 1356.877 kb on - strand at 1356.881 kb on + strand at 1356.881 kb on + strand at 1356.889 kb on - strand at 1356.889 kb on - strand at 1356.896 kb on + strand at 1356.935 kb on + strand at 1356.943 kb on + strand at 1357.073 kb on + strand at 1357.081 kb on - strand at 1357.081 kb on - strand at 1357.093 kb on + strand at 1357.208 kb on - strand, within BBR_RS16075 at 1357.208 kb on - strand, within BBR_RS16075 at 1357.307 kb on + strand, within BBR_RS16075 at 1357.315 kb on + strand, within BBR_RS16075 at 1357.323 kb on - strand, within BBR_RS16075 at 1357.411 kb on + strand, within BBR_RS16075 at 1357.411 kb on + strand, within BBR_RS16075 at 1357.411 kb on + strand, within BBR_RS16075 at 1357.528 kb on + strand, within BBR_RS16075 at 1357.550 kb on + strand, within BBR_RS16075 at 1357.587 kb on - strand, within BBR_RS16075 at 1357.707 kb on - strand, within BBR_RS16075 at 1357.860 kb on - strand at 1357.908 kb on + strand at 1358.076 kb on + strand, within BBR_RS16080 at 1358.134 kb on - strand, within BBR_RS16080 at 1358.163 kb on + strand, within BBR_RS16080 at 1358.186 kb on - strand, within BBR_RS16080 at 1358.294 kb on + strand at 1358.315 kb on - strand at 1358.352 kb on + strand at 1358.369 kb on + strand at 1358.417 kb on + strand at 1358.552 kb on - strand at 1358.597 kb on + strand at 1358.601 kb on + strand at 1358.628 kb on - strand at 1358.628 kb on - strand at 1358.628 kb on - strand at 1358.717 kb on - strand at 1358.717 kb on - strand at 1358.732 kb on + strand at 1358.764 kb on + strand at 1358.767 kb on - strand at 1358.767 kb on - strand at 1358.913 kb on - strand, within BBR_RS16085
Per-strain Table
Position Strand Gene LocusTag Fraction CDM1 without Valine remove 1,356,114 - BBR_RS16065 0.74 -0.4 1,356,149 - BBR_RS16065 0.84 -1.8 1,356,166 + BBR_RS16065 0.88 -0.3 1,356,174 - +0.8 1,356,356 + BBR_RS16070 0.20 +0.3 1,356,399 + BBR_RS16070 0.30 -0.8 1,356,529 - BBR_RS16070 0.60 -0.0 1,356,587 + BBR_RS16070 0.73 -0.5 1,356,587 + BBR_RS16070 0.73 -0.2 1,356,619 + BBR_RS16070 0.81 -1.7 1,356,634 + BBR_RS16070 0.84 -0.2 1,356,736 + +0.5 1,356,877 - -3.3 1,356,881 + +0.3 1,356,881 + -0.1 1,356,889 - -2.2 1,356,889 - -2.5 1,356,896 + -1.2 1,356,935 + -0.9 1,356,943 + -1.2 1,357,073 + -0.3 1,357,081 - -0.3 1,357,081 - -1.9 1,357,093 + -2.7 1,357,208 - BBR_RS16075 0.21 -2.2 1,357,208 - BBR_RS16075 0.21 -1.8 1,357,307 + BBR_RS16075 0.32 -1.0 1,357,315 + BBR_RS16075 0.33 +0.8 1,357,323 - BBR_RS16075 0.34 -3.3 1,357,411 + BBR_RS16075 0.44 +2.0 1,357,411 + BBR_RS16075 0.44 -0.8 1,357,411 + BBR_RS16075 0.44 -0.7 1,357,528 + BBR_RS16075 0.57 -1.7 1,357,550 + BBR_RS16075 0.59 -1.6 1,357,587 - BBR_RS16075 0.63 -3.2 1,357,707 - BBR_RS16075 0.77 -1.2 1,357,860 - -0.8 1,357,908 + +0.4 1,358,076 + BBR_RS16080 0.49 +1.2 1,358,134 - BBR_RS16080 0.65 +0.2 1,358,163 + BBR_RS16080 0.73 +0.9 1,358,186 - BBR_RS16080 0.79 -3.7 1,358,294 + -0.6 1,358,315 - -0.4 1,358,352 + -2.0 1,358,369 + +0.7 1,358,417 + +1.7 1,358,552 - -1.2 1,358,597 + -1.0 1,358,601 + +0.2 1,358,628 - +0.3 1,358,628 - +0.6 1,358,628 - -1.7 1,358,717 - +0.5 1,358,717 - +0.5 1,358,732 + +0.2 1,358,764 + +0.7 1,358,767 - +0.2 1,358,767 - +0.4 1,358,913 - BBR_RS16085 0.19 -1.6
Or see this region's nucleotide sequence