Experiment: CDM1 without Valine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS15395 and BBR_RS15400 are separated by 88 nucleotides BBR_RS15400 and BBR_RS15405 are separated by 248 nucleotides BBR_RS15405 and BBR_RS15410 are separated by 145 nucleotides
BBR_RS15395: BBR_RS15395 - tRNA (adenine-N1)-methyltransferase, at 1,222,856 to 1,223,926
_RS15395
BBR_RS15400: BBR_RS15400 - phosphohistidine phosphatase, at 1,224,015 to 1,224,572
_RS15400
BBR_RS15405: BBR_RS15405 - 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase, at 1,224,821 to 1,227,124
_RS15405
BBR_RS15410: BBR_RS15410 - methylenetetrahydrofolate reductase [NAD(P)H], at 1,227,270 to 1,228,124
_RS15410
Position (kb)
1224
1225
1226
1227
1228 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1223.874 kb on - strand at 1223.941 kb on + strand at 1223.977 kb on + strand at 1223.982 kb on + strand at 1224.086 kb on + strand, within BBR_RS15400 at 1224.094 kb on - strand, within BBR_RS15400 at 1224.121 kb on - strand, within BBR_RS15400 at 1224.145 kb on + strand, within BBR_RS15400 at 1224.155 kb on + strand, within BBR_RS15400 at 1224.155 kb on + strand, within BBR_RS15400 at 1224.155 kb on + strand, within BBR_RS15400 at 1224.163 kb on - strand, within BBR_RS15400 at 1224.163 kb on - strand, within BBR_RS15400 at 1224.181 kb on - strand, within BBR_RS15400 at 1224.192 kb on - strand, within BBR_RS15400 at 1224.342 kb on - strand, within BBR_RS15400 at 1224.355 kb on + strand, within BBR_RS15400 at 1224.355 kb on + strand, within BBR_RS15400 at 1224.358 kb on - strand, within BBR_RS15400 at 1224.358 kb on - strand, within BBR_RS15400 at 1224.365 kb on + strand, within BBR_RS15400 at 1224.365 kb on + strand, within BBR_RS15400 at 1224.379 kb on + strand, within BBR_RS15400 at 1224.398 kb on + strand, within BBR_RS15400 at 1224.401 kb on - strand, within BBR_RS15400 at 1224.426 kb on - strand, within BBR_RS15400 at 1224.441 kb on - strand, within BBR_RS15400 at 1224.442 kb on + strand, within BBR_RS15400 at 1224.442 kb on + strand, within BBR_RS15400 at 1224.442 kb on + strand, within BBR_RS15400 at 1224.450 kb on - strand, within BBR_RS15400 at 1224.450 kb on - strand, within BBR_RS15400 at 1224.450 kb on - strand, within BBR_RS15400 at 1224.450 kb on - strand, within BBR_RS15400 at 1224.496 kb on + strand, within BBR_RS15400 at 1224.571 kb on - strand at 1224.579 kb on - strand at 1224.597 kb on - strand at 1224.597 kb on - strand at 1224.621 kb on - strand at 1224.705 kb on - strand at 1224.705 kb on - strand at 1224.737 kb on - strand at 1224.847 kb on - strand at 1224.868 kb on - strand at 1224.872 kb on + strand at 1224.917 kb on - strand at 1224.917 kb on - strand at 1224.933 kb on - strand at 1225.025 kb on - strand at 1225.038 kb on + strand at 1225.038 kb on + strand at 1225.083 kb on + strand, within BBR_RS15405 at 1225.140 kb on + strand, within BBR_RS15405 at 1225.182 kb on + strand, within BBR_RS15405 at 1225.197 kb on - strand, within BBR_RS15405 at 1225.225 kb on + strand, within BBR_RS15405 at 1225.247 kb on - strand, within BBR_RS15405 at 1225.267 kb on + strand, within BBR_RS15405 at 1225.274 kb on - strand, within BBR_RS15405 at 1225.307 kb on + strand, within BBR_RS15405 at 1225.307 kb on + strand, within BBR_RS15405 at 1225.330 kb on + strand, within BBR_RS15405 at 1225.338 kb on + strand, within BBR_RS15405 at 1225.346 kb on - strand, within BBR_RS15405 at 1225.370 kb on + strand, within BBR_RS15405 at 1225.375 kb on + strand, within BBR_RS15405 at 1225.375 kb on + strand, within BBR_RS15405 at 1225.375 kb on + strand, within BBR_RS15405 at 1225.383 kb on - strand, within BBR_RS15405 at 1225.446 kb on + strand, within BBR_RS15405 at 1225.454 kb on + strand, within BBR_RS15405 at 1225.506 kb on - strand, within BBR_RS15405 at 1225.506 kb on - strand, within BBR_RS15405 at 1225.539 kb on + strand, within BBR_RS15405 at 1225.540 kb on + strand, within BBR_RS15405 at 1225.543 kb on - strand, within BBR_RS15405 at 1225.547 kb on - strand, within BBR_RS15405 at 1225.560 kb on - strand, within BBR_RS15405 at 1225.560 kb on - strand, within BBR_RS15405 at 1225.585 kb on + strand, within BBR_RS15405 at 1225.622 kb on + strand, within BBR_RS15405 at 1225.622 kb on + strand, within BBR_RS15405 at 1225.623 kb on + strand, within BBR_RS15405 at 1225.623 kb on + strand, within BBR_RS15405 at 1225.631 kb on - strand, within BBR_RS15405 at 1225.654 kb on + strand, within BBR_RS15405 at 1225.655 kb on + strand, within BBR_RS15405 at 1225.752 kb on + strand, within BBR_RS15405 at 1225.760 kb on - strand, within BBR_RS15405 at 1225.770 kb on + strand, within BBR_RS15405 at 1225.789 kb on - strand, within BBR_RS15405 at 1225.812 kb on + strand, within BBR_RS15405 at 1225.877 kb on - strand, within BBR_RS15405 at 1225.892 kb on + strand, within BBR_RS15405 at 1225.917 kb on - strand, within BBR_RS15405 at 1225.951 kb on + strand, within BBR_RS15405 at 1225.980 kb on + strand, within BBR_RS15405 at 1225.980 kb on + strand, within BBR_RS15405 at 1225.986 kb on - strand, within BBR_RS15405 at 1225.998 kb on + strand, within BBR_RS15405 at 1226.055 kb on - strand, within BBR_RS15405 at 1226.093 kb on + strand, within BBR_RS15405 at 1226.153 kb on + strand, within BBR_RS15405 at 1226.191 kb on + strand, within BBR_RS15405 at 1226.199 kb on - strand, within BBR_RS15405 at 1226.210 kb on + strand, within BBR_RS15405 at 1226.225 kb on - strand, within BBR_RS15405 at 1226.244 kb on - strand, within BBR_RS15405 at 1226.285 kb on - strand, within BBR_RS15405 at 1226.373 kb on + strand, within BBR_RS15405 at 1226.382 kb on - strand, within BBR_RS15405 at 1226.393 kb on - strand, within BBR_RS15405 at 1226.418 kb on + strand, within BBR_RS15405 at 1226.473 kb on + strand, within BBR_RS15405 at 1226.473 kb on + strand, within BBR_RS15405 at 1226.473 kb on + strand, within BBR_RS15405 at 1226.473 kb on + strand, within BBR_RS15405 at 1226.473 kb on + strand, within BBR_RS15405 at 1226.473 kb on + strand, within BBR_RS15405 at 1226.473 kb on + strand, within BBR_RS15405 at 1226.473 kb on + strand, within BBR_RS15405 at 1226.473 kb on + strand, within BBR_RS15405 at 1226.481 kb on - strand, within BBR_RS15405 at 1226.481 kb on - strand, within BBR_RS15405 at 1226.481 kb on - strand, within BBR_RS15405 at 1226.481 kb on - strand, within BBR_RS15405 at 1226.481 kb on - strand, within BBR_RS15405 at 1226.481 kb on - strand, within BBR_RS15405 at 1226.481 kb on - strand, within BBR_RS15405 at 1226.528 kb on + strand, within BBR_RS15405 at 1226.532 kb on + strand, within BBR_RS15405 at 1226.540 kb on - strand, within BBR_RS15405 at 1226.561 kb on - strand, within BBR_RS15405 at 1226.635 kb on + strand, within BBR_RS15405 at 1226.745 kb on + strand, within BBR_RS15405 at 1226.764 kb on + strand, within BBR_RS15405 at 1226.764 kb on + strand, within BBR_RS15405 at 1226.767 kb on - strand, within BBR_RS15405 at 1226.768 kb on - strand, within BBR_RS15405 at 1226.774 kb on + strand, within BBR_RS15405 at 1227.019 kb on - strand at 1227.019 kb on - strand at 1227.019 kb on - strand at 1227.032 kb on + strand at 1227.035 kb on + strand at 1227.071 kb on - strand at 1227.077 kb on - strand at 1227.077 kb on - strand at 1227.099 kb on - strand at 1227.184 kb on + strand at 1227.189 kb on + strand at 1227.189 kb on + strand at 1227.331 kb on - strand at 1227.341 kb on - strand at 1227.355 kb on - strand at 1227.368 kb on - strand, within BBR_RS15410 at 1227.409 kb on - strand, within BBR_RS15410 at 1227.418 kb on + strand, within BBR_RS15410 at 1227.492 kb on - strand, within BBR_RS15410 at 1227.527 kb on - strand, within BBR_RS15410 at 1227.591 kb on - strand, within BBR_RS15410 at 1227.621 kb on + strand, within BBR_RS15410 at 1227.682 kb on - strand, within BBR_RS15410 at 1227.698 kb on + strand, within BBR_RS15410 at 1227.759 kb on + strand, within BBR_RS15410 at 1227.902 kb on - strand, within BBR_RS15410 at 1227.905 kb on + strand, within BBR_RS15410 at 1227.934 kb on - strand, within BBR_RS15410 at 1227.934 kb on - strand, within BBR_RS15410 at 1227.986 kb on + strand, within BBR_RS15410 at 1228.002 kb on - strand, within BBR_RS15410 at 1228.027 kb on + strand, within BBR_RS15410 at 1228.044 kb on - strand at 1228.061 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction CDM1 without Valine remove 1,223,874 - +1.7 1,223,941 + +0.4 1,223,977 + -0.2 1,223,982 + -1.2 1,224,086 + BBR_RS15400 0.13 +0.5 1,224,094 - BBR_RS15400 0.14 -0.3 1,224,121 - BBR_RS15400 0.19 +1.7 1,224,145 + BBR_RS15400 0.23 +0.3 1,224,155 + BBR_RS15400 0.25 -2.7 1,224,155 + BBR_RS15400 0.25 -0.6 1,224,155 + BBR_RS15400 0.25 +0.2 1,224,163 - BBR_RS15400 0.27 +0.0 1,224,163 - BBR_RS15400 0.27 +1.0 1,224,181 - BBR_RS15400 0.30 +1.7 1,224,192 - BBR_RS15400 0.32 +0.3 1,224,342 - BBR_RS15400 0.59 +0.7 1,224,355 + BBR_RS15400 0.61 -0.3 1,224,355 + BBR_RS15400 0.61 +0.1 1,224,358 - BBR_RS15400 0.61 +1.8 1,224,358 - BBR_RS15400 0.61 +0.5 1,224,365 + BBR_RS15400 0.63 -0.2 1,224,365 + BBR_RS15400 0.63 -1.9 1,224,379 + BBR_RS15400 0.65 -0.1 1,224,398 + BBR_RS15400 0.69 +0.9 1,224,401 - BBR_RS15400 0.69 +0.0 1,224,426 - BBR_RS15400 0.74 +1.2 1,224,441 - BBR_RS15400 0.76 +1.0 1,224,442 + BBR_RS15400 0.77 +0.2 1,224,442 + BBR_RS15400 0.77 +0.0 1,224,442 + BBR_RS15400 0.77 -0.3 1,224,450 - BBR_RS15400 0.78 +1.5 1,224,450 - BBR_RS15400 0.78 -0.4 1,224,450 - BBR_RS15400 0.78 +0.6 1,224,450 - BBR_RS15400 0.78 -0.6 1,224,496 + BBR_RS15400 0.86 -0.4 1,224,571 - +0.0 1,224,579 - +1.3 1,224,597 - +0.7 1,224,597 - +1.2 1,224,621 - -0.5 1,224,705 - +0.3 1,224,705 - -1.2 1,224,737 - +1.6 1,224,847 - -1.2 1,224,868 - -1.9 1,224,872 + -2.0 1,224,917 - -2.6 1,224,917 - -1.1 1,224,933 - +0.5 1,225,025 - -2.0 1,225,038 + +0.7 1,225,038 + +1.1 1,225,083 + BBR_RS15405 0.11 +1.3 1,225,140 + BBR_RS15405 0.14 -2.0 1,225,182 + BBR_RS15405 0.16 -0.4 1,225,197 - BBR_RS15405 0.16 -0.7 1,225,225 + BBR_RS15405 0.18 -2.8 1,225,247 - BBR_RS15405 0.18 +0.6 1,225,267 + BBR_RS15405 0.19 -0.5 1,225,274 - BBR_RS15405 0.20 +0.3 1,225,307 + BBR_RS15405 0.21 -3.9 1,225,307 + BBR_RS15405 0.21 -1.2 1,225,330 + BBR_RS15405 0.22 -0.4 1,225,338 + BBR_RS15405 0.22 -1.5 1,225,346 - BBR_RS15405 0.23 -0.4 1,225,370 + BBR_RS15405 0.24 -2.0 1,225,375 + BBR_RS15405 0.24 -2.7 1,225,375 + BBR_RS15405 0.24 -1.1 1,225,375 + BBR_RS15405 0.24 -2.3 1,225,383 - BBR_RS15405 0.24 +0.3 1,225,446 + BBR_RS15405 0.27 -1.5 1,225,454 + BBR_RS15405 0.27 -1.1 1,225,506 - BBR_RS15405 0.30 -1.4 1,225,506 - BBR_RS15405 0.30 +1.7 1,225,539 + BBR_RS15405 0.31 -1.0 1,225,540 + BBR_RS15405 0.31 -1.9 1,225,543 - BBR_RS15405 0.31 -1.6 1,225,547 - BBR_RS15405 0.32 -0.2 1,225,560 - BBR_RS15405 0.32 -0.8 1,225,560 - BBR_RS15405 0.32 -0.8 1,225,585 + BBR_RS15405 0.33 -2.3 1,225,622 + BBR_RS15405 0.35 -2.3 1,225,622 + BBR_RS15405 0.35 -3.0 1,225,623 + BBR_RS15405 0.35 -2.4 1,225,623 + BBR_RS15405 0.35 -1.4 1,225,631 - BBR_RS15405 0.35 -2.0 1,225,654 + BBR_RS15405 0.36 -1.2 1,225,655 + BBR_RS15405 0.36 -0.4 1,225,752 + BBR_RS15405 0.40 -3.5 1,225,760 - BBR_RS15405 0.41 +0.3 1,225,770 + BBR_RS15405 0.41 -2.5 1,225,789 - BBR_RS15405 0.42 -1.6 1,225,812 + BBR_RS15405 0.43 -1.4 1,225,877 - BBR_RS15405 0.46 -1.6 1,225,892 + BBR_RS15405 0.46 -1.6 1,225,917 - BBR_RS15405 0.48 +0.5 1,225,951 + BBR_RS15405 0.49 -0.9 1,225,980 + BBR_RS15405 0.50 -3.6 1,225,980 + BBR_RS15405 0.50 -3.6 1,225,986 - BBR_RS15405 0.51 -0.0 1,225,998 + BBR_RS15405 0.51 -2.8 1,226,055 - BBR_RS15405 0.54 -1.2 1,226,093 + BBR_RS15405 0.55 -1.5 1,226,153 + BBR_RS15405 0.58 -1.1 1,226,191 + BBR_RS15405 0.59 -0.8 1,226,199 - BBR_RS15405 0.60 -0.3 1,226,210 + BBR_RS15405 0.60 -1.1 1,226,225 - BBR_RS15405 0.61 -0.4 1,226,244 - BBR_RS15405 0.62 -0.6 1,226,285 - BBR_RS15405 0.64 +1.3 1,226,373 + BBR_RS15405 0.67 -2.9 1,226,382 - BBR_RS15405 0.68 -2.1 1,226,393 - BBR_RS15405 0.68 -0.9 1,226,418 + BBR_RS15405 0.69 -2.9 1,226,473 + BBR_RS15405 0.72 -1.3 1,226,473 + BBR_RS15405 0.72 -3.5 1,226,473 + BBR_RS15405 0.72 -1.5 1,226,473 + BBR_RS15405 0.72 -1.8 1,226,473 + BBR_RS15405 0.72 -3.4 1,226,473 + BBR_RS15405 0.72 -1.4 1,226,473 + BBR_RS15405 0.72 -1.9 1,226,473 + BBR_RS15405 0.72 -2.7 1,226,473 + BBR_RS15405 0.72 -3.7 1,226,481 - BBR_RS15405 0.72 +0.4 1,226,481 - BBR_RS15405 0.72 -0.0 1,226,481 - BBR_RS15405 0.72 -1.5 1,226,481 - BBR_RS15405 0.72 -1.8 1,226,481 - BBR_RS15405 0.72 -1.5 1,226,481 - BBR_RS15405 0.72 -2.1 1,226,481 - BBR_RS15405 0.72 -1.4 1,226,528 + BBR_RS15405 0.74 -1.7 1,226,532 + BBR_RS15405 0.74 -3.7 1,226,540 - BBR_RS15405 0.75 -0.0 1,226,561 - BBR_RS15405 0.76 -0.4 1,226,635 + BBR_RS15405 0.79 -1.0 1,226,745 + BBR_RS15405 0.84 -0.9 1,226,764 + BBR_RS15405 0.84 -1.6 1,226,764 + BBR_RS15405 0.84 -1.5 1,226,767 - BBR_RS15405 0.84 +0.1 1,226,768 - BBR_RS15405 0.85 -1.0 1,226,774 + BBR_RS15405 0.85 -3.3 1,227,019 - -2.2 1,227,019 - -1.5 1,227,019 - -2.0 1,227,032 + -0.4 1,227,035 + -2.1 1,227,071 - -1.4 1,227,077 - -0.3 1,227,077 - -1.3 1,227,099 - +0.1 1,227,184 + -1.3 1,227,189 + -1.6 1,227,189 + -2.4 1,227,331 - +0.3 1,227,341 - +1.1 1,227,355 - +0.1 1,227,368 - BBR_RS15410 0.11 +0.4 1,227,409 - BBR_RS15410 0.16 +0.9 1,227,418 + BBR_RS15410 0.17 +0.9 1,227,492 - BBR_RS15410 0.26 -1.4 1,227,527 - BBR_RS15410 0.30 -0.0 1,227,591 - BBR_RS15410 0.38 +0.9 1,227,621 + BBR_RS15410 0.41 +1.3 1,227,682 - BBR_RS15410 0.48 -1.7 1,227,698 + BBR_RS15410 0.50 +0.7 1,227,759 + BBR_RS15410 0.57 +1.2 1,227,902 - BBR_RS15410 0.74 -1.1 1,227,905 + BBR_RS15410 0.74 -0.3 1,227,934 - BBR_RS15410 0.78 +1.7 1,227,934 - BBR_RS15410 0.78 +0.9 1,227,986 + BBR_RS15410 0.84 -0.7 1,228,002 - BBR_RS15410 0.86 +0.3 1,228,027 + BBR_RS15410 0.89 +1.5 1,228,044 - -0.6 1,228,061 + +0.9
Or see this region's nucleotide sequence