Experiment: NAG (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2132 and Shewana3_2133 are separated by 277 nucleotides Shewana3_2133 and Shewana3_2134 are separated by 118 nucleotides Shewana3_2134 and Shewana3_2135 are separated by 71 nucleotides Shewana3_2135 and Shewana3_2136 are separated by 271 nucleotides
Shewana3_2132: Shewana3_2132 - succinylglutamate desuccinylase (RefSeq), at 2,557,823 to 2,558,857
_2132
Shewana3_2133: Shewana3_2133 - peptide methionine sulfoxide reductase (RefSeq), at 2,559,135 to 2,559,614
_2133
Shewana3_2134: Shewana3_2134 - phosphoglucomutase (RefSeq), at 2,559,733 to 2,561,385
_2134
Shewana3_2135: Shewana3_2135 - replication initiation regulator SeqA (RefSeq), at 2,561,457 to 2,562,008
_2135
Shewana3_2136: Shewana3_2136 - alpha/beta hydrolase fold (RefSeq), at 2,562,280 to 2,563,080
_2136
Position (kb)
2559
2560
2561
2562 Strain fitness (log2 ratio)
-2
-1
0
1 at 2558.803 kb on - strand at 2559.042 kb on + strand at 2559.210 kb on + strand, within Shewana3_2133 at 2559.506 kb on - strand, within Shewana3_2133 at 2559.561 kb on + strand, within Shewana3_2133 at 2561.465 kb on - strand at 2562.111 kb on - strand at 2562.211 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction NAG (C) remove 2,558,803 - -2.1 2,559,042 + -0.2 2,559,210 + Shewana3_2133 0.16 -0.1 2,559,506 - Shewana3_2133 0.77 -0.3 2,559,561 + Shewana3_2133 0.89 +0.7 2,561,465 - +0.9 2,562,111 - -0.4 2,562,211 - +0.7
Or see this region's nucleotide sequence